Current Protocols in Pharmacology, volume 44, issue 1

Overview of High‐Throughput Screening

Entzeroth Michael ¹

Flotow Horst ¹

Condron Peter ¹

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Experimental Therapeutics Centre, Agency for Science, Technology, and Research (A^*STAR) Singapore |

Publication type: Journal Article

Publication date: 2009-03-10

Wiley

Journal: Current Protocols in Pharmacology

Quartile SCImago

Quartile WOS

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Impact factor: —

ISSN: 19348282, 19348290

DOI: 10.1002/0471141755.ph0904s44

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Pharmacology

Abstract

High‐throughput screening (HTS) is a key process used in drug discovery to identify hits from compound libraries that may become leads for medicinal chemistry optimization. This updated overview discusses the utilization of compound libraries, compounds derived from combinatorial and parallel synthesis campaigns and natural product sources; creation of mother and daughter plates; and compound storage, handling, and bar coding in HTS. The unit also presents an overview of established and emerging assay technologies (i.e., time‐resolved fluorescence, fluorescence polarization, fluorescence‐correlation spectroscopy, functional whole cell assays, and high‐content assays) and their integration in automation hardware and IT systems. This revised unit provides updated descriptions of state‐of‐the‐art instrumentation and technologies in this rapidly changing environment. The section on assay methodologies now also covers enzyme complementation assays and methods for high‐throughput screening of ion channel activities. Finally, a section on criteria for assay robustness is included discussing the Z′‐factor, which is now a widely accepted criterion for evaluation and validation of high throughput screening assays. Curr. Protoc. Pharmacol. 44:9.4.1‐9.4.27. © 2009 by John Wiley & Sons, Inc.

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Citations by journals

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SLAS Discovery	SLAS Discovery, 3, 6.82% SLAS Discovery 3 publications, 6.82%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 4.55% International Journal of Molecular Sciences 2 publications, 4.55%
Current Medicinal Chemistry	Current Medicinal Chemistry, 2, 4.55% Current Medicinal Chemistry 2 publications, 4.55%
Scientific Reports	Scientific Reports, 2, 4.55% Scientific Reports 2 publications, 4.55%
Methods in Molecular Biology	Methods in Molecular Biology, 2, 4.55% Methods in Molecular Biology 2 publications, 4.55%
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Molecules	Molecules, 1, 2.27% Molecules 1 publication, 2.27%
Sensors	Sensors, 1, 2.27% Sensors 1 publication, 2.27%
Antibiotics	Antibiotics, 1, 2.27% Antibiotics 1 publication, 2.27%
Journal of Pharmaceutical Innovation	Journal of Pharmaceutical Innovation, 1, 2.27% Journal of Pharmaceutical Innovation 1 publication, 2.27%
Journal of Cheminformatics	Journal of Cheminformatics, 1, 2.27% Journal of Cheminformatics 1 publication, 2.27%
Drug Discovery Today	Drug Discovery Today, 1, 2.27% Drug Discovery Today 1 publication, 2.27%
Acta Pharmaceutica Sinica B	Acta Pharmaceutica Sinica B, 1, 2.27% Acta Pharmaceutica Sinica B 1 publication, 2.27%
Current Research in Biotechnology	Current Research in Biotechnology, 1, 2.27% Current Research in Biotechnology 1 publication, 2.27%
PLoS Neglected Tropical Diseases	PLoS Neglected Tropical Diseases, 1, 2.27% PLoS Neglected Tropical Diseases 1 publication, 2.27%
New Biotechnology	New Biotechnology, 1, 2.27% New Biotechnology 1 publication, 2.27%
Medicinal Research Reviews	Medicinal Research Reviews, 1, 2.27% Medicinal Research Reviews 1 publication, 2.27%
Journal of Biotechnology	Journal of Biotechnology, 1, 2.27% Journal of Biotechnology 1 publication, 2.27%
Analytica Chimica Acta	Analytica Chimica Acta, 1, 2.27% Analytica Chimica Acta 1 publication, 2.27%
Patterns	Patterns, 1, 2.27% Patterns 1 publication, 2.27%
Cytometry. Part A : the journal of the International Society for Analytical Cytology	Cytometry. Part A : the journal of the International Society for Analytical Cytology, 1, 2.27% Cytometry. Part A : the journal of the International Society for Analytical Cytology 1 publication, 2.27%
Current Protocols in Pharmacology	Current Protocols in Pharmacology, 1, 2.27% Current Protocols in Pharmacology 1 publication, 2.27%
Advanced Energy Materials	Advanced Energy Materials, 1, 2.27% Advanced Energy Materials 1 publication, 2.27%
Current Protocols in Stem Cell Biology	Current Protocols in Stem Cell Biology, 1, 2.27% Current Protocols in Stem Cell Biology 1 publication, 2.27%
Clinical Pharmacology in Drug Development	Clinical Pharmacology in Drug Development, 1, 2.27% Clinical Pharmacology in Drug Development 1 publication, 2.27%
RSC Advances	RSC Advances, 1, 2.27% RSC Advances 1 publication, 2.27%
Journal of Cancer Prevention & Current Research	Journal of Cancer Prevention & Current Research, 1, 2.27% Journal of Cancer Prevention & Current Research 1 publication, 2.27%
Expert Opinion on Drug Discovery	Expert Opinion on Drug Discovery, 1, 2.27% Expert Opinion on Drug Discovery 1 publication, 2.27%
New Zealand Journal of Zoology	New Zealand Journal of Zoology, 1, 2.27% New Zealand Journal of Zoology 1 publication, 2.27%
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Citations by publishers

	1 2 3 4 5 6 7 8 9
Elsevier	Elsevier, 9, 20.45% Elsevier 9 publications, 20.45%
Springer Nature	Springer Nature, 7, 15.91% Springer Nature 7 publications, 15.91%
Wiley	Wiley, 7, 15.91% Wiley 7 publications, 15.91%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 5, 11.36% Multidisciplinary Digital Publishing Institute (MDPI) 5 publications, 11.36%
Bentham Science	Bentham Science, 3, 6.82% Bentham Science 3 publications, 6.82%
SAGE	SAGE, 3, 6.82% SAGE 3 publications, 6.82%
Taylor & Francis	Taylor & Francis, 2, 4.55% Taylor & Francis 2 publications, 4.55%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 1, 2.27% Public Library of Science (PLoS) 1 publication, 2.27%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 2.27% Royal Society of Chemistry (RSC) 1 publication, 2.27%
MedCrave Group Kft.	MedCrave Group Kft., 1, 2.27% MedCrave Group Kft. 1 publication, 2.27%
Paediatrician Publishers LLC	Paediatrician Publishers LLC, 1, 2.27% Paediatrician Publishers LLC 1 publication, 2.27%
Frontiers Media S.A.	Frontiers Media S.A., 1, 2.27% Frontiers Media S.A. 1 publication, 2.27%
	1 2 3 4 5 6 7 8 9

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Entzeroth M., Flotow H., Condron P. Overview of High‐Throughput Screening // Current Protocols in Pharmacology. 2009. Vol. 44. No. 1.

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Entzeroth M., Flotow H., Condron P. Overview of High‐Throughput Screening // Current Protocols in Pharmacology. 2009. Vol. 44. No. 1.

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RIS Copy

TY - JOUR

DO - 10.1002/0471141755.ph0904s44

UR - https://doi.org/10.1002%2F0471141755.ph0904s44

TI - Overview of High‐Throughput Screening

T2 - Current Protocols in Pharmacology

AU - Entzeroth, Michael

AU - Flotow, Horst

AU - Condron, Peter

PY - 2009

DA - 2009/03/10 00:00:00

PB - Wiley

IS - 1

VL - 44

SN - 1934-8282

SN - 1934-8290

ER -

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@article{2009_Entzeroth,

author = {Michael Entzeroth and Horst Flotow and Peter Condron},

title = {Overview of High‐Throughput Screening},

journal = {Current Protocols in Pharmacology},

year = {2009},

volume = {44},

publisher = {Wiley},

month = {mar},

url = {https://doi.org/10.1002%2F0471141755.ph0904s44},

number = {1},

doi = {10.1002/0471141755.ph0904s44}

}

Found error?

Publisher

Wiley

Journal

Current Protocols in Pharmacology

Quartile SCImago

Quartile WOS

—

Impact factor

—

ISSN

19348282 (Print)
19348290 (Electronic)