Biotechnology and Bioengineering, volume 119, issue 3, pages 1033-1042
Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication
Minin A.S.
1, 2
,
Blatov Igor
3
,
Lebedeva Valeria
3
,
Tiukhai Maxim
4
,
Pozdina Varvara
5
,
Byzov Ilya
1
,
Zubarev I
3, 6
3
4
Laboratory of Experimental Radiobiology Ural Research Center for Radiation Medicine Chelyabinsk Russian Federation
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Publication type: Journal Article
Publication date: 2022-01-19
Journal:
Biotechnology and Bioengineering
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 3.8
ISSN: 00063592, 10970290
Applied Microbiology and Biotechnology
Biotechnology
Bioengineering
Abstract
In vitro systems serve as compact and manipulate models to investigate interactions between different cell types. A homogeneous population of cells predictably and uniformly responds to external factors. In a heterogeneous cell population, the effect of external growth factors is perceived in the context of intercellular interactions. Indirect cell cocultivation allows one to observe the paracrine effects of cells and separately analyze cell populations. The article describes an application of custom‐made cell cocultivation systems based on protein membranes separated from the bottom of the vessel by the 3D printed holder or kept afloat by a magnetic field. Using the proposed cocultivation system, we analyzed the interaction of A549 cells and fibroblasts, in the presence and absence of growth factors. During cocultivation of cells, the expression of genes of the activation for epithelial and mesenchymal transitions decreases. The article proposes the application of a newly available system for the cocultivation of different cell types.
Citations by journals
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Fermentation
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Fermentation
1 publication, 100%
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 100%
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Minin A. et al. Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication // Biotechnology and Bioengineering. 2022. Vol. 119. No. 3. pp. 1033-1042.
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Minin A., Blatov I., Lebedeva V., Tiukhai M., Pozdina V., Byzov I., Zubarev I. Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication // Biotechnology and Bioengineering. 2022. Vol. 119. No. 3. pp. 1033-1042.
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TY - JOUR
DO - 10.1002/bit.28031
UR - https://doi.org/10.1002%2Fbit.28031
TI - Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication
T2 - Biotechnology and Bioengineering
AU - Blatov, Igor
AU - Lebedeva, Valeria
AU - Tiukhai, Maxim
AU - Pozdina, Varvara
AU - Byzov, Ilya
AU - Minin, A.S.
AU - Zubarev, I
PY - 2022
DA - 2022/01/19 00:00:00
PB - Wiley
SP - 1033-1042
IS - 3
VL - 119
SN - 0006-3592
SN - 1097-0290
ER -
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@article{2022_Minin,
author = {Igor Blatov and Valeria Lebedeva and Maxim Tiukhai and Varvara Pozdina and Ilya Byzov and A.S. Minin and I Zubarev},
title = {Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication},
journal = {Biotechnology and Bioengineering},
year = {2022},
volume = {119},
publisher = {Wiley},
month = {jan},
url = {https://doi.org/10.1002%2Fbit.28031},
number = {3},
pages = {1033--1042},
doi = {10.1002/bit.28031}
}
Cite this
MLA
Copy
Minin, A.S., et al. “Novel cost-efficient protein-based membrane system for cells cocultivation and modeling the intercellular communication.” Biotechnology and Bioengineering, vol. 119, no. 3, Jan. 2022, pp. 1033-1042. https://doi.org/10.1002%2Fbit.28031.