Electrophoresis, volume 38, issue 19, pages 2536-2541
Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers
Furman Christophe
1, 2
,
Carpentier Rodolphe
1, 3, 4
,
Barczyk Amélie
1, 2, 5
,
Chavatte Philippe
1, 2, 5
,
Betbeder Didier
1, 3, 4
,
Lipka E.
1, 6
1
Inserm; U995-LIRIC Lille France
|
2
Faculté de Pharmacie, Plateforme de Binding; Université de Lille; U995-LIRIC Lille France
|
3
CHRU de Lille; U995-LIRIC France
|
4
Faculté de Médecine; Université de Lille; U995-LIRIC Lille France
|
5
Faculté de Pharmacie; Institut de Chimie A. Lespagnol; Université de Lille; U995-LIRIC Lille France
|
6
Faculté de Pharmacie; Laboratoire de Chimie Analytique; Université de Lille; U995-LIRIC Lille France
|
Publication type: Journal Article
Publication date: 2017-04-28
Journal:
Electrophoresis
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 2.9
ISSN: 01730835, 15222683
Biochemistry
Clinical Biochemistry
Analytical Chemistry
Abstract
A reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method has been developed and validated for the quantification of paclitaxel encapsulated in biodegradable poly(lactic‐co‐glycolic) (PLGA) copolymer nanoparticles. This simple (isocratic mode, without additive) and rapid (retention time of the paclitaxel under 4 min) methodology permits the detection of low quantities of paclitaxel in nanoparticulate formulations and the determination of the encapsulation efficiency (EE). Analysis was achieved on an octadecyl stationary phase. The isocratic mobile phase consisted of acetonitrile:water 80:20 (v/v) (flow rate = 0.8 mL/min). Stability of free paclitaxel was preliminary studied in those chromatographic conditions. The calibration curve was linear in the concentration range of 2–10 μg/mL (R2 = 0.9994). The method was specific with valuable trueness, repeatability (intra‐day precision) and intermediate precision (inter‐day precision) based on relative standard deviation (RSD) values (less than 2%). The limits of detection (LOD) and quantification (LOQ) were 0.56 and 1.85 ng/mL, respectively. This developed method was successfully employed for quantifying paclitaxel in PLGA 50:50 co‐polymer nanoparticles. The accurate knowledge of the encapsulated paclitaxel concentration is essential to define the quantities of PLGA nanoparticles necessary to achieve the in vitro cell viability study.
Citations by journals
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1
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Annales Pharmaceutiques Francaises
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1 publication, 14.29%
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Chromatographia
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International Journal of Pharmaceutics
1 publication, 14.29%
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Microchemical Journal
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Microchemical Journal
1 publication, 14.29%
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Critical Reviews in Analytical Chemistry
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1 publication, 14.29%
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1
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Citations by publishers
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1
2
3
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|
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Elsevier
|
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3 publications, 42.86%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 14.29%
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Frontiers Media S.A.
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1 publication, 14.29%
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Springer Nature
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Springer Nature
1 publication, 14.29%
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Taylor & Francis
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Taylor & Francis
1 publication, 14.29%
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1
2
3
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Furman C. et al. Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers // Electrophoresis. 2017. Vol. 38. No. 19. pp. 2536-2541.
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Furman C., Carpentier R., Barczyk A., Chavatte P., Betbeder D., Lipka E. Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers // Electrophoresis. 2017. Vol. 38. No. 19. pp. 2536-2541.
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TY - JOUR
DO - 10.1002/elps.201600552
UR - https://doi.org/10.1002%2Felps.201600552
TI - Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers
T2 - Electrophoresis
AU - Furman, Christophe
AU - Barczyk, Amélie
AU - Chavatte, Philippe
AU - Betbeder, Didier
AU - Lipka, E.
AU - Carpentier, Rodolphe
PY - 2017
DA - 2017/04/28 00:00:00
PB - Wiley
SP - 2536-2541
IS - 19
VL - 38
SN - 0173-0835
SN - 1522-2683
ER -
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@article{2017_Furman,
author = {Christophe Furman and Amélie Barczyk and Philippe Chavatte and Didier Betbeder and E. Lipka and Rodolphe Carpentier},
title = {Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers},
journal = {Electrophoresis},
year = {2017},
volume = {38},
publisher = {Wiley},
month = {apr},
url = {https://doi.org/10.1002%2Felps.201600552},
number = {19},
pages = {2536--2541},
doi = {10.1002/elps.201600552}
}
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Furman, Christophe, et al. “Development and validation of a reversed‐phase HPLC method for the quantification of paclitaxel in different PLGA nanocarriers.” Electrophoresis, vol. 38, no. 19, Apr. 2017, pp. 2536-2541. https://doi.org/10.1002%2Felps.201600552.