Journal of Applied Toxicology, volume 39, issue 1, pages 38-71
In vitro assays and techniques utilized in anticancer drug discovery
1
Institute of Biochemistry, Molecular Biology and Biotechnology; University of Colombo; Colombo 03 Sri Lanka
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Publication type: Journal Article
Publication date: 2018-08-02
Journal:
Journal of Applied Toxicology
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.3
ISSN: 0260437X, 10991263
DOI:
10.1002/jat.3658
Toxicology
Abstract
Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.
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1
2
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Citations by publishers
2
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12
14
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2
4
6
8
10
12
14
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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EDIRIWEERA M. K., TENNEKOON K. H., SAMARAKOON S. R. In vitro assays and techniques utilized in anticancer drug discovery // Journal of Applied Toxicology. 2018. Vol. 39. No. 1. pp. 38-71.
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EDIRIWEERA M. K., TENNEKOON K. H., SAMARAKOON S. R. In vitro assays and techniques utilized in anticancer drug discovery // Journal of Applied Toxicology. 2018. Vol. 39. No. 1. pp. 38-71.
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TY - JOUR
DO - 10.1002/jat.3658
UR - https://doi.org/10.1002%2Fjat.3658
TI - In vitro assays and techniques utilized in anticancer drug discovery
T2 - Journal of Applied Toxicology
AU - EDIRIWEERA, MERAN KESHAWA
AU - TENNEKOON, KAMANI H.
AU - SAMARAKOON, SAMEERA RANGANATH
PY - 2018
DA - 2018/08/02 00:00:00
PB - Wiley
SP - 38-71
IS - 1
VL - 39
SN - 0260-437X
SN - 1099-1263
ER -
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@article{2018_EDIRIWEERA
author = {MERAN KESHAWA EDIRIWEERA and KAMANI H. TENNEKOON and SAMEERA RANGANATH SAMARAKOON},
title = {In vitro assays and techniques utilized in anticancer drug discovery},
journal = {Journal of Applied Toxicology},
year = {2018},
volume = {39},
publisher = {Wiley},
month = {aug},
url = {https://doi.org/10.1002%2Fjat.3658},
number = {1},
pages = {38--71},
doi = {10.1002/jat.3658}
}
Cite this
MLA
Copy
EDIRIWEERA, MERAN KESHAWA, et al. “In vitro assays and techniques utilized in anticancer drug discovery.” Journal of Applied Toxicology, vol. 39, no. 1, Aug. 2018, pp. 38-71. https://doi.org/10.1002%2Fjat.3658.