Microscopy Research and Technique, volume 85, issue 2, pages 562-569

Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles

Bagrov Dmitry V. 1, 2
Glukhov Grigory S. 2
Moiseenko Andrey V. 2, 3
Karlova Maria G. 2
Litvinov Daniil S. 2
Zaitsev Petr А. 2
Shishova Anna A. 4, 5
Kovpak Anastasia A. 4
Ivin Yury Y. 4
Piniaeva Anastasia N. 4
Oksanich Alexey S. 6
Volok Viktor P. 2, 4
Osolodkin Dmitry I. 1, 4, 5
Egorov Alexey M. 2, 3, 6
Kirpichnikov Mikhail P. 2
Publication typeJournal Article
Publication date2021-09-09
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor2.5
ISSN1059910X, 10970029
PubMed ID:  34498784
Instrumentation
Histology
Medical Laboratory Technology
Anatomy
Abstract

The severe COVID‐19 pandemic drives the research toward the SARS‐CoV‐2 virion structure and the possible therapies against it. Here, we characterized the β‐propiolactone inactivated SARS‐CoV‐2 virions using transmission electron microscopy (TEM) and atomic force microscopy (AFM). We compared the SARS‐CoV‐2 samples purified by two consecutive chromatographic procedures (size exclusion chromatography [SEC], followed by ion‐exchange chromatography [IEC]) with samples purified by ultracentrifugation. The samples prepared using SEC and IEC retained more spikes on the surface than the ones prepared using ultracentrifugation, as confirmed by TEM and AFM. TEM showed that the spike (S) proteins were in the pre‐fusion conformation. Notably, the S proteins could be recognized by specific monoclonal antibodies. Analytical TEM showed that the inactivated virions retained nucleic acid. Altogether, we demonstrated that the inactivated SARS‐CoV‐2 virions retain the structural features of native viruses and provide a prospective vaccine candidate.

Citations by journals

1
2
Viruses
Viruses, 2, 14.29%
Viruses
2 publications, 14.29%
Voprosy Virusologii
Voprosy Virusologii, 1, 7.14%
Voprosy Virusologii
1 publication, 7.14%
Emerging Microbes and Infections
Emerging Microbes and Infections, 1, 7.14%
Emerging Microbes and Infections
1 publication, 7.14%
Vaccines
Vaccines, 1, 7.14%
Vaccines
1 publication, 7.14%
Advanced Fiber Materials
Advanced Fiber Materials, 1, 7.14%
Advanced Fiber Materials
1 publication, 7.14%
Journal of Chromatography A
Journal of Chromatography A, 1, 7.14%
Journal of Chromatography A
1 publication, 7.14%
Acta Biomaterialia
Acta Biomaterialia, 1, 7.14%
Acta Biomaterialia
1 publication, 7.14%
Microscopy Research and Technique
Microscopy Research and Technique, 1, 7.14%
Microscopy Research and Technique
1 publication, 7.14%
Biomedicines
Biomedicines, 1, 7.14%
Biomedicines
1 publication, 7.14%
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika), 1, 7.14%
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
1 publication, 7.14%
Medical academic journal
Medical academic journal, 1, 7.14%
Medical academic journal
1 publication, 7.14%
Biophysics (Russian Federation)
Biophysics (Russian Federation), 1, 7.14%
Biophysics (Russian Federation)
1 publication, 7.14%
Pharmaceutics
Pharmaceutics, 1, 7.14%
Pharmaceutics
1 publication, 7.14%
1
2

Citations by publishers

1
2
3
4
5
Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 5, 35.71%
Multidisciplinary Digital Publishing Institute (MDPI)
5 publications, 35.71%
Springer Nature
Springer Nature, 2, 14.29%
Springer Nature
2 publications, 14.29%
Elsevier
Elsevier, 2, 14.29%
Elsevier
2 publications, 14.29%
Central Research Institute for Epidemiology
Central Research Institute for Epidemiology, 1, 7.14%
Central Research Institute for Epidemiology
1 publication, 7.14%
Wiley
Wiley, 1, 7.14%
Wiley
1 publication, 7.14%
National Association of the Specialists in Control of Health Care-Associated Infections
National Association of the Specialists in Control of Health Care-Associated Infections, 1, 7.14%
National Association of the Specialists in Control of Health Care-Associated Infections
1 publication, 7.14%
Eco-Vector LLC
Eco-Vector LLC, 1, 7.14%
Eco-Vector LLC
1 publication, 7.14%
Pleiades Publishing
Pleiades Publishing, 1, 7.14%
Pleiades Publishing
1 publication, 7.14%
1
2
3
4
5
  • We do not take into account publications that without a DOI.
  • Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
  • Statistics recalculated weekly.
Metrics
Share
Cite this
GOST |
Cite this
GOST Copy
Bagrov D. V. et al. Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles // Microscopy Research and Technique. 2021. Vol. 85. No. 2. pp. 562-569.
GOST all authors (up to 50) Copy
Bagrov D. V., Glukhov G. S., Moiseenko A. V., Karlova M. G., Litvinov D. S., Zaitsev P. А., Kozlovskaya L. I., Shishova A. A., Kovpak A. A., Ivin Y. Y., Piniaeva A. N., Oksanich A. S., Volok V. P., Osolodkin D. I., Ishmukhametov A. A., Egorov A. M., Shaitan K. V., Kirpichnikov M. P., Sokolova O. S. Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles // Microscopy Research and Technique. 2021. Vol. 85. No. 2. pp. 562-569.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1002/jemt.23931
UR - https://doi.org/10.1002%2Fjemt.23931
TI - Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles
T2 - Microscopy Research and Technique
AU - Bagrov, Dmitry V.
AU - Glukhov, Grigory S.
AU - Moiseenko, Andrey V.
AU - Karlova, Maria G.
AU - Litvinov, Daniil S.
AU - Zaitsev, Petr А.
AU - Kozlovskaya, Liubov I.
AU - Shishova, Anna A.
AU - Kovpak, Anastasia A.
AU - Ivin, Yury Y.
AU - Piniaeva, Anastasia N.
AU - Oksanich, Alexey S.
AU - Volok, Viktor P.
AU - Osolodkin, Dmitry I.
AU - Ishmukhametov, Aydar A.
AU - Egorov, Alexey M.
AU - Shaitan, Konstantin V.
AU - Kirpichnikov, Mikhail P.
AU - Sokolova, Olga S.
PY - 2021
DA - 2021/09/09 00:00:00
PB - Wiley
SP - 562-569
IS - 2
VL - 85
PMID - 34498784
SN - 1059-910X
SN - 1097-0029
ER -
BibTex |
Cite this
BibTex Copy
@article{2021_Bagrov
author = {Dmitry V. Bagrov and Grigory S. Glukhov and Andrey V. Moiseenko and Maria G. Karlova and Daniil S. Litvinov and Petr А. Zaitsev and Liubov I. Kozlovskaya and Anna A. Shishova and Anastasia A. Kovpak and Yury Y. Ivin and Anastasia N. Piniaeva and Alexey S. Oksanich and Viktor P. Volok and Dmitry I. Osolodkin and Aydar A. Ishmukhametov and Alexey M. Egorov and Konstantin V. Shaitan and Mikhail P. Kirpichnikov and Olga S. Sokolova},
title = {Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles},
journal = {Microscopy Research and Technique},
year = {2021},
volume = {85},
publisher = {Wiley},
month = {sep},
url = {https://doi.org/10.1002%2Fjemt.23931},
number = {2},
pages = {562--569},
doi = {10.1002/jemt.23931}
}
MLA
Cite this
MLA Copy
Bagrov, Dmitry V., et al. “Structural characterization of β‐propiolactone inactivated severe acute respiratory syndrome coronavirus 2 ( SARS‐CoV ‐2) particles.” Microscopy Research and Technique, vol. 85, no. 2, Sep. 2021, pp. 562-569. https://doi.org/10.1002%2Fjemt.23931.
Found error?