Journal of Molecular Recognition, volume 29, issue 6, pages 242-247
TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site
Azhibek Dulat M
1, 2
,
Skvortsov Dmitry
1
,
Andreeva Anna
3
,
Zatsepin Timofei S.
1, 2
,
Arutyunyan Alexandr
1
,
Zvereva Maria
1
,
Publication type: Journal Article
Publication date: 2015-12-16
Journal:
Journal of Molecular Recognition
Quartile SCImago
Q4
Quartile WOS
Q3
Impact factor: 2.7
ISSN: 09523499, 10991352
DOI:
10.1002/jmr.2521
Molecular Biology
Structural Biology
Abstract
Telomerase is a key component of the telomere length maintenance system in the majority of eukaryotes. Telomerase displays maximal activity in stem and cancer cells with high proliferative potential. In humans, telomerase activity is regulated by various mechanisms, including the interaction with telomere ssDNA overhangs that contain a repetitive G‐rich sequence, and with noncoding RNA, Telomeric repeat‐containing RNA (TERRA), that contains the same sequence. So these nucleic acids can compete for telomerase RNA templates in the cell. In this study, we have investigated the ability of different model substrates mimicking telomere DNA overhangs and TERRA RNA to compete for telomerase in vitro through a previously developed telomerase inhibitor assay. We have shown in this study that RNA oligonucleotides are better competitors for telomerase that DNA ones as RNA also use an alternative binding site on telomerase, and the presence of 2′‐OH groups is significant in these interactions. In contrast to DNA, the possibility of forming intramolecular G‐quadruplex structures has a minor effect for RNA binding to telomerase. Taking together our data, we propose that TERRA RNA binds better to telomerase compared with its native substrate – the 3′‐end of telomere DNA overhang. As a result, some specific factor may exist that participates in switching telomerase from TERRA to the 3′‐end of DNA for telomere elongation at the distinct period of a cell cycle in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
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Azhibek D. M. et al. TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site // Journal of Molecular Recognition. 2015. Vol. 29. No. 6. pp. 242-247.
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Azhibek D. M., Skvortsov D., Andreeva A., Zatsepin T. S., Arutyunyan A., Zvereva M., Dontsova O. TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site // Journal of Molecular Recognition. 2015. Vol. 29. No. 6. pp. 242-247.
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TY - JOUR
DO - 10.1002/jmr.2521
UR - https://doi.org/10.1002%2Fjmr.2521
TI - TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site
T2 - Journal of Molecular Recognition
AU - Andreeva, Anna
AU - Arutyunyan, Alexandr
AU - Dontsova, Olga
AU - Azhibek, Dulat M
AU - Skvortsov, Dmitry
AU - Zatsepin, Timofei S.
AU - Zvereva, Maria
PY - 2015
DA - 2015/12/16 00:00:00
PB - Wiley
SP - 242-247
IS - 6
VL - 29
SN - 0952-3499
SN - 1099-1352
ER -
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@article{2015_Azhibek,
author = {Anna Andreeva and Alexandr Arutyunyan and Olga Dontsova and Dulat M Azhibek and Dmitry Skvortsov and Timofei S. Zatsepin and Maria Zvereva},
title = {TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site},
journal = {Journal of Molecular Recognition},
year = {2015},
volume = {29},
publisher = {Wiley},
month = {dec},
url = {https://doi.org/10.1002%2Fjmr.2521},
number = {6},
pages = {242--247},
doi = {10.1002/jmr.2521}
}
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MLA
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Azhibek, Dulat M., et al. “TERRA mimicking ssRNAs prevail over the DNA substrate for telomerase in vitro due to interactions with the alternative binding site.” Journal of Molecular Recognition, vol. 29, no. 6, Dec. 2015, pp. 242-247. https://doi.org/10.1002%2Fjmr.2521.