Open Access
Open access
MicrobiologyOpen, volume 9, issue 7

Interaction of two strongly divergent archaellins stabilizes the structure of theHalorubrumarchaellum

Papke R. Thane 3
Kireev I. 4
Сурин А.К. 1, 5, 6
Fedorov Oleg V. 1
Publication typeJournal Article
Publication date2020-04-21
Journal: MicrobiologyOpen
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor3.4
ISSN20458827
PubMed ID:  32352651
Microbiology
Abstract
Halophilic archaea from the genus Halorubrum possess two extraordinarily diverged archaellin genes, flaB1 and flaB2. To clarify roles for each archaellin, we compared two natural Halorubrum lacusprofundi strains: One of them contains both archaellin genes, and the other has the flaB2 gene only. Both strains synthesize functional archaella; however, the strain, where both archaellins are present, is more motile. In addition, we expressed these archaellins in a Haloferax volcanii strain from which the endogenous archaellin genes were deleted. Three Hfx. volcanii strains expressing Hrr. lacusprofundi archaellins produced functional filaments consisting of only one (FlaB1 or FlaB2) or both (FlaB1/FlaB2) archaellins. All three strains were motile, although there were profound differences in the efficiency of motility. Both native and recombinant FlaB1/FlaB2 filaments have greater thermal stability and resistance to low salinity stress than single‐component filaments. Functional supercoiled Hrr. lacusprofundi archaella can be composed of either single archaellin: FlaB2 or FlaB1; however, the two divergent archaellin subunits provide additional stabilization to the archaellum structure and thus adaptation to a wider range of external conditions. Comparative genomic analysis suggests that the described combination of divergent archaellins is not restricted to Hrr. lacusprofundi, but is occurring also in organisms from other haloarchaeal genera.

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Pyatibratov M. G. et al. Interaction of two strongly divergent archaellins stabilizes the structure of theHalorubrumarchaellum // MicrobiologyOpen. 2020. Vol. 9. No. 7.
GOST all authors (up to 50) Copy
Pyatibratov M. G., Syutkin A. S., Quax T. E., Melnik T. N., Papke R. T., Gogarten J. P., Kireev I., Сурин А., Beznosov S. N., Galeva A. V., Fedorov O. V. Interaction of two strongly divergent archaellins stabilizes the structure of theHalorubrumarchaellum // MicrobiologyOpen. 2020. Vol. 9. No. 7.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1002/mbo3.1047
UR - https://doi.org/10.1002%2Fmbo3.1047
TI - Interaction of two strongly divergent archaellins stabilizes the structure of theHalorubrumarchaellum
T2 - MicrobiologyOpen
AU - Fedorov, Oleg V.
AU - Pyatibratov, Mikhail G
AU - Syutkin, Alexey S
AU - Quax, Tessa E.F.
AU - Melnik, Tatiana N
AU - Gogarten, Johann Peter
AU - Kireev, I.
AU - Сурин, А.К.
AU - Beznosov, Sergei N
AU - Galeva, Anna V
AU - Papke, R. Thane
PY - 2020
DA - 2020/04/21 00:00:00
PB - Wiley
IS - 7
VL - 9
PMID - 32352651
SN - 2045-8827
ER -
BibTex
Cite this
BibTex Copy
@article{2020_Pyatibratov
author = {Oleg V. Fedorov and Mikhail G Pyatibratov and Alexey S Syutkin and Tessa E.F. Quax and Tatiana N Melnik and Johann Peter Gogarten and I. Kireev and А.К. Сурин and Sergei N Beznosov and Anna V Galeva and R. Thane Papke},
title = {Interaction of two strongly divergent archaellins stabilizes the structure of theHalorubrumarchaellum},
journal = {MicrobiologyOpen},
year = {2020},
volume = {9},
publisher = {Wiley},
month = {apr},
url = {https://doi.org/10.1002%2Fmbo3.1047},
number = {7},
doi = {10.1002/mbo3.1047}
}
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