Journal of Fluorescence, volume 27, issue 6, pages 2231-2238
Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives
Kim Dokyoung
1, 2, 3
,
Ma Donghee
4
,
Kim Muwoong
3
,
Jung Yuna
3
,
Kim Na Hee
3
,
Lee Chiho
5
,
Cho Seo Won
4
,
Park Sungnam
5
,
Huh Youngbuhm
1, 3
,
Jung Junyang
1, 3
,
Ahn Kyo Han
4
1
Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, South Korea
|
2
Center for Converging Humanities, Kyung Hee University, Seoul, South Korea
|
3
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea
|
4
Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, South Korea
|
5
Department of Chemistry, Korea University, Seoul, South Korea
|
Publication type: Journal Article
Publication date: 2017-08-19
Journal:
Journal of Fluorescence
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 2.7
ISSN: 10530509, 15734994
Biochemistry
Spectroscopy
Clinical Biochemistry
Law
Sociology and Political Science
Social Sciences (miscellaneous)
Clinical Psychology
Abstract
8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1–4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence. The chromatography and mass spectroscopy analysis clearly support the labeling of the BODIPY dye on the BSA. The protein labeling with blue-emitting BODIPY would be applicable for studying protein dynamics and fluorescence resonance energy transfer (FRET) with intrinsic biomolecules.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Kim D. et al. Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives // Journal of Fluorescence. 2017. Vol. 27. No. 6. pp. 2231-2238.
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Kim D., Ma D., Kim M., Jung Y., Kim N. H., Lee C., Cho S. W., Park S., Huh Y., Jung J., Ahn K. H. Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives // Journal of Fluorescence. 2017. Vol. 27. No. 6. pp. 2231-2238.
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TY - JOUR
DO - 10.1007/s10895-017-2164-5
UR - https://doi.org/10.1007%2Fs10895-017-2164-5
TI - Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives
T2 - Journal of Fluorescence
AU - Kim, Dokyoung
AU - Ma, Donghee
AU - Kim, Muwoong
AU - Jung, Yuna
AU - Kim, Na Hee
AU - Lee, Chiho
AU - Cho, Seo Won
AU - Park, Sungnam
AU - Huh, Youngbuhm
AU - Jung, Junyang
AU - Ahn, Kyo Han
PY - 2017
DA - 2017/08/19 00:00:00
PB - Springer Nature
SP - 2231-2238
IS - 6
VL - 27
SN - 1053-0509
SN - 1573-4994
ER -
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@article{2017_Kim,
author = {Dokyoung Kim and Donghee Ma and Muwoong Kim and Yuna Jung and Na Hee Kim and Chiho Lee and Seo Won Cho and Sungnam Park and Youngbuhm Huh and Junyang Jung and Kyo Han Ahn},
title = {Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives},
journal = {Journal of Fluorescence},
year = {2017},
volume = {27},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1007%2Fs10895-017-2164-5},
number = {6},
pages = {2231--2238},
doi = {10.1007/s10895-017-2164-5}
}
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Kim, Dokyoung, et al. “Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives.” Journal of Fluorescence, vol. 27, no. 6, Aug. 2017, pp. 2231-2238. https://doi.org/10.1007%2Fs10895-017-2164-5.