Open Access
3 Biotech, volume 8, issue 1, publication number 3
Optimization of a resazurin-based microplate assay for large-scale compound screenings against Klebsiella pneumoniae
Kim Hyung Jun
1
,
Jang Soojin
1
1
Antibacterial Resistance Research Laboratory, Discovery Biology Department, Institut Pasteur Korea, Seongnam-si, South Korea
|
Publication type: Journal Article
Publication date: 2017-12-07
PubMed ID:
29242763
Biotechnology
Agricultural and Biological Sciences (miscellaneous)
Environmental Science (miscellaneous)
Abstract
A new resazurin-based assay was evaluated and optimized using a microplate (384-well) format for high-throughput screening of antibacterial molecules against Klebsiella pneumoniae. Growth of the bacteria in 384-well plates was more effectively measured and had a > sixfold higher signal-to-background ratio using the resazurin-based assay compared with absorbance measurements at 600 nm. Determination of minimum inhibitory concentrations of the antibiotics revealed that the optimized assay quantitatively measured antibacterial activity of various antibiotics. An edge effect observed in the initial assay was significantly reduced using a 1-h incubation of the bacteria-containing plates at room temperature. There was an approximately 10% decrease in signal variability between the edge and the middle wells along with improvement in the assay robustness (Z′ = 0.99). This optimized resazurin-based assay is an efficient, inexpensive, and robust assay that can quantitatively measure antibacterial activity using a high-throughput screening system to assess a large number of compounds for discovery of new antibiotics against K. pneumoniae.
Citations by journals
|
1
|
|
|
Combinatorial Chemistry and High Throughput Screening
|
Combinatorial Chemistry and High Throughput Screening
1 publication, 8.33%
|
|
Microbial Drug Resistance
|
Microbial Drug Resistance
1 publication, 8.33%
|
|
PLoS ONE
|
PLoS ONE
1 publication, 8.33%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
1 publication, 8.33%
|
|
Applied Sciences (Switzerland)
|
Applied Sciences (Switzerland)
1 publication, 8.33%
|
|
Frontiers in Cellular and Infection Microbiology
|
Frontiers in Cellular and Infection Microbiology
1 publication, 8.33%
|
|
Journal of Nanobiotechnology
|
Journal of Nanobiotechnology
1 publication, 8.33%
|
|
Toxicology in Vitro
|
Toxicology in Vitro
1 publication, 8.33%
|
|
Biochemistry and Biophysics Reports
|
Biochemistry and Biophysics Reports
1 publication, 8.33%
|
|
Medical Journal of Cell Biology
|
Medical Journal of Cell Biology
1 publication, 8.33%
|
|
BIO-PROTOCOL
|
BIO-PROTOCOL, 1, 8.33%
BIO-PROTOCOL
1 publication, 8.33%
|
|
Methods in Enzymology
|
Methods in Enzymology
1 publication, 8.33%
|
|
1
|
Citations by publishers
|
1
2
3
|
|
|
Elsevier
|
Elsevier
3 publications, 25%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 16.67%
|
|
Bentham Science
|
Bentham Science
1 publication, 8.33%
|
|
Mary Ann Liebert
|
Mary Ann Liebert
1 publication, 8.33%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
1 publication, 8.33%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 8.33%
|
|
Springer Nature
|
Springer Nature
1 publication, 8.33%
|
|
Walter de Gruyter
|
Walter de Gruyter
1 publication, 8.33%
|
|
Bio-Protocol
|
Bio-Protocol, 1, 8.33%
Bio-Protocol
1 publication, 8.33%
|
|
1
2
3
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2019,2020,2021,2022,2023],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[3,4,1,3,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["25","33.33","8.33","25","8.33"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Combinatorial Chemistry and High Throughput Screening","Microbial Drug Resistance","PLoS ONE","International Journal of Molecular Sciences","Applied Sciences (Switzerland)","Frontiers in Cellular and Infection Microbiology","Journal of Nanobiotechnology","Toxicology in Vitro","Biochemistry and Biophysics Reports","Medical Journal of Cell Biology","BIO-PROTOCOL","Methods in Enzymology"],"ids":[13844,4802,24963,14627,16650,21709,11794,19840,20408,1847,26240,24566],"codes":[0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/doUaFUZdxUEQjLi1TwZjGHi8HXYNWWSk04dSC6Xh_medium.webp","\/storage\/images\/resized\/Zy5YST233rzRbZP24I24MzZm64hz20ZV9qQopmMn_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp","","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[8.33,8.33,8.33,8.33,8.33,8.33,8.33,8.33,8.33,8.33,8.33,8.33],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Bentham Science","Mary Ann Liebert","Public Library of Science (PLoS)","Frontiers Media S.A.","Springer Nature","Walter de Gruyter","Bio-Protocol"],"ids":[17,202,39,230,344,208,8,4,7047],"codes":[0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/doUaFUZdxUEQjLi1TwZjGHi8HXYNWWSk04dSC6Xh_medium.webp","\/storage\/images\/resized\/Zy5YST233rzRbZP24I24MzZm64hz20ZV9qQopmMn_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/3SpVxcYL33bOvPq4sHxJLH2NeKNeDloahSUpNiO4_medium.webp",""],"datasets":[{"label":"","data":[3,2,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[25,16.67,8.33,8.33,8.33,8.33,8.33,8.33,8.33],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Kim H. J., Jang S. Optimization of a resazurin-based microplate assay for large-scale compound screenings against Klebsiella pneumoniae // 3 Biotech. 2017. Vol. 8. No. 1. 3
GOST all authors (up to 50)
Copy
Kim H. J., Jang S. Optimization of a resazurin-based microplate assay for large-scale compound screenings against Klebsiella pneumoniae // 3 Biotech. 2017. Vol. 8. No. 1. 3
Cite this
RIS
Copy
TY - JOUR
DO - 10.1007/s13205-017-1034-9
UR - https://doi.org/10.1007%2Fs13205-017-1034-9
TI - Optimization of a resazurin-based microplate assay for large-scale compound screenings against Klebsiella pneumoniae
T2 - 3 Biotech
AU - Kim, Hyung Jun
AU - Jang, Soojin
PY - 2017
DA - 2017/12/07 00:00:00
PB - Springer Nature
IS - 1
VL - 8
PMID - 29242763
SN - 2190-572X
SN - 2190-5738
ER -
Cite this
BibTex
Copy
@article{2017_Kim,
author = {Hyung Jun Kim and Soojin Jang},
title = {Optimization of a resazurin-based microplate assay for large-scale compound screenings against Klebsiella pneumoniae},
journal = {3 Biotech},
year = {2017},
volume = {8},
publisher = {Springer Nature},
month = {dec},
url = {https://doi.org/10.1007%2Fs13205-017-1034-9},
number = {1},
doi = {10.1007/s13205-017-1034-9}
}