Open Access
Biophysics Reports, volume 3, issue 4-6, pages 64-72
Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells
1
Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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2
Department of Pathology, MitoCare Center, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, USA
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Publication type: Journal Article
Publication date: 2017-03-24
Journal:
Biophysics Reports
Quartile SCImago
— Quartile WOS
—
Impact factor: —
ISSN: 23643439, 23643420
PubMed ID:
29238743
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering
Abstract
Mitochondrial dynamics refers to the processes maintaining mitochondrial homeostasis, including mitochondrial fission, fusion, transport, biogenesis, and mitophagy. Mitochondrial dynamics is essential for maintaining the metabolic function of mitochondria as well as their regulatory roles in cell signaling. In this review, we summarize the recently developed imaging techniques for studying mitochondrial dynamics including: mitochondrial-targeted fluorescent proteins and dyes, live-cell imaging using photoactivation, photoswitching and cell fusion, mitochondrial transcription and replication imaging by in situ hybridization, and imaging mitochondrial dynamics by super-resolution microscopy. Moreover, we discuss examples of how to choose and combine proper fluorescent dyes and/or proteins.
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Citations by publishers
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1 publication, 5.26%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Liu X. et al. Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells // Biophysics Reports. 2017. Vol. 3. No. 4-6. pp. 64-72.
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Liu X., Yang L., Long Q., Weaver D., Hajnóczky G. Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells // Biophysics Reports. 2017. Vol. 3. No. 4-6. pp. 64-72.
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TY - JOUR
DO - 10.1007/s41048-017-0037-8
UR - https://doi.org/10.1007%2Fs41048-017-0037-8
TI - Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells
T2 - Biophysics Reports
AU - Liu, Xingguo
AU - Yang, Liang
AU - Long, Qi
AU - Weaver, David
AU - Hajnóczky, György
PY - 2017
DA - 2017/03/24 00:00:00
PB - Biophysical Society of China
SP - 64-72
IS - 4-6
VL - 3
PMID - 29238743
SN - 2364-3439
SN - 2364-3420
ER -
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@article{2017_Liu,
author = {Xingguo Liu and Liang Yang and Qi Long and David Weaver and György Hajnóczky},
title = {Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells},
journal = {Biophysics Reports},
year = {2017},
volume = {3},
publisher = {Biophysical Society of China},
month = {mar},
url = {https://doi.org/10.1007%2Fs41048-017-0037-8},
number = {4-6},
pages = {64--72},
doi = {10.1007/s41048-017-0037-8}
}
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MLA
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Liu, Xingguo, et al. “Choosing proper fluorescent dyes, proteins, and imaging techniques to study mitochondrial dynamics in mammalian cells.” Biophysics Reports, vol. 3, no. 4-6, Mar. 2017, pp. 64-72. https://doi.org/10.1007%2Fs41048-017-0037-8.