,
volume 488
,
issue 1
,
pages 36-42
The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography
Publication type: Journal Article
Publication date: 1977-07-01
SJR: —
CiteScore: —
Impact factor: —
ISSN: 00052760
PubMed ID:
560868
Biochemistry
Biophysics
Endocrinology
Abstract
Phosphatidylserine has been prepared from phosphatidylcholine by a one-step transphosphatidylation catalyzed by phospholipase D in the presence of L-serine. The resulting mixture of phosphatidylserine and phosphatidic acid is easily and rapidly separated by CM-cellulose column chromatography using step=wise elution with solvents containing increasing percentages of methanol in chloroform. The over-all yield of the procedure is 40-50% depending on the scale of the preparation. CM-Cellulose column chromatography proved to be extremely useful in separating phospholipid mixtures obtained by phosphatidyltransferase reactions of phospholipase D and is also suitable for fractionation of other lipid extracts.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
20
40
60
80
100
120
|
|
|
Biochimica et Biophysica Acta - Biomembranes
111 publications, 25.11%
|
|
|
Journal of Biological Chemistry
57 publications, 12.9%
|
|
|
Biochemistry
52 publications, 11.76%
|
|
|
Chemistry and Physics of Lipids
31 publications, 7.01%
|
|
|
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
19 publications, 4.3%
|
|
|
FEBS Letters
11 publications, 2.49%
|
|
|
Journal of Lipid Research
10 publications, 2.26%
|
|
|
Biochemical and Biophysical Research Communications
9 publications, 2.04%
|
|
|
Methods in Enzymology
6 publications, 1.36%
|
|
|
Lipids
5 publications, 1.13%
|
|
|
Thrombosis and Haemostasis
4 publications, 0.9%
|
|
|
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
3 publications, 0.68%
|
|
|
Analytical Biochemistry
3 publications, 0.68%
|
|
|
Biophysical Journal
3 publications, 0.68%
|
|
|
Archives of Biochemistry and Biophysics
3 publications, 0.68%
|
|
|
JAOCS, Journal of the American Oil Chemists' Society
3 publications, 0.68%
|
|
|
Journal of Fermentation and Bioengineering
2 publications, 0.45%
|
|
|
Analytica Chimica Acta
2 publications, 0.45%
|
|
|
Bioorganic and Medicinal Chemistry Letters
2 publications, 0.45%
|
|
|
Journal of Electroanalytical Chemistry
2 publications, 0.45%
|
|
|
Tetrahedron Letters
2 publications, 0.45%
|
|
|
Biochimie
2 publications, 0.45%
|
|
|
Chemico-Biological Interactions
2 publications, 0.45%
|
|
|
Thrombosis Research
2 publications, 0.45%
|
|
|
Journal of Colloid and Interface Science
2 publications, 0.45%
|
|
|
Berichte der Bunsengesellschaft für physikalische Chemie
2 publications, 0.45%
|
|
|
Rapid Communications in Mass Spectrometry
2 publications, 0.45%
|
|
|
Methods in Molecular Biology
2 publications, 0.45%
|
|
|
Life Sciences
1 publication, 0.23%
|
|
|
20
40
60
80
100
120
|
Publishers
|
50
100
150
200
250
|
|
|
Elsevier
223 publications, 50.45%
|
|
|
American Society for Biochemistry and Molecular Biology
67 publications, 15.16%
|
|
|
American Chemical Society (ACS)
53 publications, 11.99%
|
|
|
Wiley
28 publications, 6.33%
|
|
|
Springer Nature
15 publications, 3.39%
|
|
|
Taylor & Francis
5 publications, 1.13%
|
|
|
Georg Thieme Verlag KG
4 publications, 0.9%
|
|
|
Biophysical Society
3 publications, 0.68%
|
|
|
The American Association of Immunologists
1 publication, 0.23%
|
|
|
The Company of Biologists
1 publication, 0.23%
|
|
|
SAGE
1 publication, 0.23%
|
|
|
MDPI
1 publication, 0.23%
|
|
|
Han-Gug Misaengmul Hag-hoe/The Microbiological Society of Korea
1 publication, 0.23%
|
|
|
1 publication, 0.23%
|
|
|
American Society of Hematology
1 publication, 0.23%
|
|
|
50
100
150
200
250
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
442
Total citations:
442
Citations from 2024:
0
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Comfurius P., Zwaal R. F. A. The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography // Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1977. Vol. 488. No. 1. pp. 36-42.
GOST all authors (up to 50)
Copy
Comfurius P., Zwaal R. F. A. The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography // Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1977. Vol. 488. No. 1. pp. 36-42.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/0005-2760(77)90120-5
UR - https://doi.org/10.1016/0005-2760(77)90120-5
TI - The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography
T2 - Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
AU - Comfurius, P.
AU - Zwaal, R F A
PY - 1977
DA - 1977/07/01
PB - Elsevier
SP - 36-42
IS - 1
VL - 488
PMID - 560868
SN - 0005-2760
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{1977_Comfurius,
author = {P. Comfurius and R F A Zwaal},
title = {The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography},
journal = {Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism},
year = {1977},
volume = {488},
publisher = {Elsevier},
month = {jul},
url = {https://doi.org/10.1016/0005-2760(77)90120-5},
number = {1},
pages = {36--42},
doi = {10.1016/0005-2760(77)90120-5}
}
Cite this
MLA
Copy
Comfurius, P., and R F A Zwaal. “The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography.” Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, vol. 488, no. 1, Jul. 1977, pp. 36-42. https://doi.org/10.1016/0005-2760(77)90120-5.