Open Access

Methods in Cell Biology, pages 77-94

Fluorescence live cell imaging

Ettinger Andreas ¹

Wittmann Torsten ¹

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Department of Cell and Tissue Biology, University of California, San Francisco, USA |

Publication type: Book Chapter

Publication date: 2014-06-25

Elsevier

Journal: Methods in Cell Biology

Quartile SCImago

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Quartile WOS

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Impact factor: —

ISSN: 0091679X

DOI: 10.1016/B978-0-12-420138-5.00005-7

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PubMed ID: 24974023

Abstract

Fluorescence microscopy of live cells has become an integral part of modern cell biology. Fluorescent protein (FP) tags, live cell dyes, and other methods to fluorescently label proteins of interest provide a range of tools to investigate virtually any cellular process under the microscope. The two main experimental challenges in collecting meaningful live cell microscopy data are to minimize photodamage while retaining a useful signal-to-noise ratio and to provide a suitable environment for cells or tissues to replicate physiological cell dynamics. This chapter aims to give a general overview on microscope design choices critical for fluorescence live cell imaging that apply to most fluorescence microscopy modalities and on environmental control with a focus on mammalian tissue culture cells. In addition, we provide guidance on how to design and evaluate FP constructs by spinning disk confocal microscopy.

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Citations by journals

	1 2 3 4 5 6 7 8
Methods in Molecular Biology	Methods in Molecular Biology, 8, 4.02% Methods in Molecular Biology 8 publications, 4.02%
Scientific Reports	Scientific Reports, 5, 2.51% Scientific Reports 5 publications, 2.51%
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Methods in Cell Biology	Methods in Cell Biology, 4, 2.01% Methods in Cell Biology 4 publications, 2.01%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 3, 1.51% International Journal of Molecular Sciences 3 publications, 1.51%
PLoS ONE	PLoS ONE, 3, 1.51% PLoS ONE 3 publications, 1.51%
The Analyst	The Analyst, 3, 1.51% The Analyst 3 publications, 1.51%
Molecular Imaging and Biology	Molecular Imaging and Biology, 2, 1.01% Molecular Imaging and Biology 2 publications, 1.01%
Journal of Cell Science	Journal of Cell Science, 2, 1.01% Journal of Cell Science 2 publications, 1.01%
Sensors	Sensors, 2, 1.01% Sensors 2 publications, 1.01%
Nanomaterials	Nanomaterials, 2, 1.01% Nanomaterials 2 publications, 1.01%
Biosensors	Biosensors, 2, 1.01% Biosensors 2 publications, 1.01%
Frontiers in Cell and Developmental Biology	Frontiers in Cell and Developmental Biology, 2, 1.01% Frontiers in Cell and Developmental Biology 2 publications, 1.01%
Stem Cell Reviews and Reports	Stem Cell Reviews and Reports, 2, 1.01% Stem Cell Reviews and Reports 2 publications, 1.01%
Journal of Nanobiotechnology	Journal of Nanobiotechnology, 2, 1.01% Journal of Nanobiotechnology 2 publications, 1.01%
Nature Communications	Nature Communications, 2, 1.01% Nature Communications 2 publications, 1.01%
Nature Protocols	Nature Protocols, 2, 1.01% Nature Protocols 2 publications, 1.01%
Communications Biology	Communications Biology, 2, 1.01% Communications Biology 2 publications, 1.01%
Chemical Reviews	Chemical Reviews, 2, 1.01% Chemical Reviews 2 publications, 1.01%
ACS Biomaterials Science and Engineering	ACS Biomaterials Science and Engineering, 2, 1.01% ACS Biomaterials Science and Engineering 2 publications, 1.01%
Organic and Biomolecular Chemistry	Organic and Biomolecular Chemistry, 2, 1.01% Organic and Biomolecular Chemistry 2 publications, 1.01%
Chemical Communications	Chemical Communications, 2, 1.01% Chemical Communications 2 publications, 1.01%
Russian Journal of Bioorganic Chemistry	Russian Journal of Bioorganic Chemistry, 2, 1.01% Russian Journal of Bioorganic Chemistry 2 publications, 1.01%
Applied Optics	Applied Optics, 2, 1.01% Applied Optics 2 publications, 1.01%
ACS Omega	ACS Omega, 2, 1.01% ACS Omega 2 publications, 1.01%
RSC Advances	RSC Advances, 1, 0.5% RSC Advances 1 publication, 0.5%
Biomicrofluidics	Biomicrofluidics, 1, 0.5% Biomicrofluidics 1 publication, 0.5%
Biochemical Society Transactions	Biochemical Society Transactions, 1, 0.5% Biochemical Society Transactions 1 publication, 0.5%
Philosophical Transactions of the Royal Society B: Biological Sciences	Philosophical Transactions of the Royal Society B: Biological Sciences, 1, 0.5% Philosophical Transactions of the Royal Society B: Biological Sciences 1 publication, 0.5%
	1 2 3 4 5 6 7 8

Citations by publishers

	5 10 15 20 25 30 35 40
Springer Nature	Springer Nature, 40, 20.1% Springer Nature 40 publications, 20.1%
Elsevier	Elsevier, 32, 16.08% Elsevier 32 publications, 16.08%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 18, 9.05% Multidisciplinary Digital Publishing Institute (MDPI) 18 publications, 9.05%
American Chemical Society (ACS)	American Chemical Society (ACS), 13, 6.53% American Chemical Society (ACS) 13 publications, 6.53%
Wiley	Wiley, 13, 6.53% Wiley 13 publications, 6.53%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 12, 6.03% Royal Society of Chemistry (RSC) 12 publications, 6.03%
Frontiers Media S.A.	Frontiers Media S.A., 5, 2.51% Frontiers Media S.A. 5 publications, 2.51%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 4, 2.01% Public Library of Science (PLoS) 4 publications, 2.01%
Pleiades Publishing	Pleiades Publishing, 3, 1.51% Pleiades Publishing 3 publications, 1.51%
Taylor & Francis	Taylor & Francis, 3, 1.51% Taylor & Francis 3 publications, 1.51%
IEEE	IEEE, 3, 1.51% IEEE 3 publications, 1.51%
The Company of Biologists	The Company of Biologists, 2, 1.01% The Company of Biologists 2 publications, 1.01%
IOP Publishing	IOP Publishing, 2, 1.01% IOP Publishing 2 publications, 1.01%
American Physiological Society	American Physiological Society, 2, 1.01% American Physiological Society 2 publications, 1.01%
Oxford University Press	Oxford University Press, 2, 1.01% Oxford University Press 2 publications, 1.01%
Optical Society of America	Optical Society of America, 2, 1.01% Optical Society of America 2 publications, 1.01%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 2, 1.01% American Association for the Advancement of Science (AAAS) 2 publications, 1.01%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 1, 0.5% American Institute of Physics (AIP) 1 publication, 0.5%
Portland Press	Portland Press, 1, 0.5% Portland Press 1 publication, 0.5%
The Royal Society	The Royal Society, 1, 0.5% The Royal Society 1 publication, 0.5%
World Scientific	World Scientific, 1, 0.5% World Scientific 1 publication, 0.5%
The Electrochemical Society	The Electrochemical Society, 1, 0.5% The Electrochemical Society 1 publication, 0.5%
PagePress	PagePress, 1, 0.5% PagePress 1 publication, 0.5%
Chemical Industry Press	Chemical Industry Press, 1, 0.5% Chemical Industry Press 1 publication, 0.5%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 1, 0.5% American Society for Biochemistry and Molecular Biology 1 publication, 0.5%
Cambridge University Press	Cambridge University Press, 1, 0.5% Cambridge University Press 1 publication, 0.5%
Walter de Gruyter	Walter de Gruyter, 1, 0.5% Walter de Gruyter 1 publication, 0.5%
Image Processing Systems Institute of RAS	Image Processing Systems Institute of RAS, 1, 0.5% Image Processing Systems Institute of RAS 1 publication, 0.5%
American Institute of Mathematical Sciences (AIMS)	American Institute of Mathematical Sciences (AIMS), 1, 0.5% American Institute of Mathematical Sciences (AIMS) 1 publication, 0.5%
	5 10 15 20 25 30 35 40

We do not take into account publications that without a DOI.
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GOST Copy

Ettinger A., Wittmann T. Fluorescence live cell imaging // Methods in Cell Biology. 2014. pp. 77-94.

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Ettinger A., Wittmann T. Fluorescence live cell imaging // Methods in Cell Biology. 2014. pp. 77-94.

RIS |

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RIS Copy

TY - GENERIC

DO - 10.1016/B978-0-12-420138-5.00005-7

UR - https://doi.org/10.1016%2FB978-0-12-420138-5.00005-7

TI - Fluorescence live cell imaging

T2 - Methods in Cell Biology

AU - Ettinger, Andreas

AU - Wittmann, Torsten

PY - 2014

DA - 2014/06/25 00:00:00

PB - Elsevier

SP - 77-94

PMID - 24974023

SN - 0091-679X

ER -

BibTex

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BibTex Copy

@incollection{2014_Ettinger,

author = {Andreas Ettinger and Torsten Wittmann},

title = {Fluorescence live cell imaging},

publisher = {Elsevier},

year = {2014},

pages = {77--94},

month = {jun}

}

Found error?

Publisher

Elsevier

Journal

Methods in Cell Biology

Quartile SCImago

—

Quartile WOS

—

Impact factor

—

ISSN

0091679X (Print)