Biochimie, volume 95, issue 12, pages 2423-2428
Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique
Zatsepin Timofei S
,
Zvereva Maria I.
,
PRASSOLOV VLADIMIR S.
2
,
Prokofjeva Maria M
,
Azhibek Dulat M
1
,
Naraikina Yulia V
3
,
Прокофьева М. М.
2
,
Zvereva Maria
1
,
Publication type: Journal Article
Publication date: 2013-12-01
Biochemistry
General Medicine
Abstract
Telomeres are the nucleoprotein complexes that cap the linear chromosome ends. Telomerase is a ribonucleoprotein that maintains telomere length in stem, embryonic and cancer cells. Somatic cells don't contain active telomerase and telomere function as mitotic clock and telomere length determines the number of cell divisions. Telomerase RNA (TER) contains the template for telomere synthesis and serves as a structural scaffold for holoenzyme assembly. We compared different oligonucleotide based methods for telomerase RNA inhibition, such as antisense oligonucleotides, knockdown by transient siRNA transfection and silencing by miRNA derived from short expressed RNA hairpin in HEK293 cells. All of these methods were applied to different TER regions. Our results revealed that CR2/CR3 domain of TER is accessible in vitro and in vivo and could serve as an optimal site for oligonucleotide-based telomerase silencing.
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Citations by publishers
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Elsevier
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Vasilkova D. V. et al. Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique // Biochimie. 2013. Vol. 95. No. 12. pp. 2423-2428.
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Vasilkova D. V., Azhibek D. M., Zatsepin T. S., Naraikina Y. V., PRASSOLOV V. S., Prokofjeva M. M., Zvereva M. I., Rubtsova M. P., Zatsepin T. S., Прокофьева М. М., Zvereva M., Rubtsova M. Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique // Biochimie. 2013. Vol. 95. No. 12. pp. 2423-2428.
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TY - JOUR
DO - 10.1016/J.BIOCHI.2013.09.001
UR - https://doi.org/10.1016%2FJ.BIOCHI.2013.09.001
TI - Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique
T2 - Biochimie
AU - Vasilkova, Daria V
AU - Azhibek, Dulat M
AU - Zatsepin, Timofei S
AU - Naraikina, Yulia V
AU - PRASSOLOV, VLADIMIR S.
AU - Prokofjeva, Maria M
AU - Zvereva, Maria I.
AU - Rubtsova, Maria P
AU - Zatsepin, Timofei S.
AU - Прокофьева, М. М.
AU - Zvereva, Maria
AU - Rubtsova, Maria
PY - 2013
DA - 2013/12/01 00:00:00
PB - Elsevier
SP - 2423-2428
IS - 12
VL - 95
SN - 0300-9084
SN - 6183-1638
ER -
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@article{2013_Vasilkova,
author = {Daria V Vasilkova and Dulat M Azhibek and Timofei S Zatsepin and Yulia V Naraikina and VLADIMIR S. PRASSOLOV and Maria M Prokofjeva and Maria I. Zvereva and Maria P Rubtsova and Timofei S. Zatsepin and М. М. Прокофьева and Maria Zvereva and Maria Rubtsova},
title = {Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique},
journal = {Biochimie},
year = {2013},
volume = {95},
publisher = {Elsevier},
month = {dec},
url = {https://doi.org/10.1016%2FJ.BIOCHI.2013.09.001},
number = {12},
pages = {2423--2428},
doi = {10.1016/J.BIOCHI.2013.09.001}
}
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Vasilkova, Daria V., et al. “Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique.” Biochimie, vol. 95, no. 12, Dec. 2013, pp. 2423-2428. https://doi.org/10.1016%2FJ.BIOCHI.2013.09.001.