Acta Biomaterialia, volume 103, pages 272-280
Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP.
Unzueta U.
1
,
Roldan Mònica
,
Pesarrodona Mireia
,
Benitez Raul
,
Sánchez-Chardi Alejandro
2
,
Conchillo Solé Oscar
1, 3
,
Mangues Ramon
,
Villaverde A.
4
,
2
Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain.
|
Publication type: Journal Article
Publication date: 2020-02-01
Biochemistry
Molecular Biology
General Medicine
Biotechnology
Biomaterials
Biomedical Engineering
Abstract
Fluorescent proteins are useful imaging and theranostic agents, but their potential superiority over alternative dyes is weakened by substantial photobleaching under irradiation. Enhancing protein photostability has been attempted through diverse strategies, with irregular results and limited applicability. In this context, we wondered if the controlled oligomerization of Green Fluorescent Protein (GFP) as nanoscale supramolecular complexes could stabilize the fluorophore through the newly formed protein-protein contacts, and thus, enhance its global photostability. For that, we have here analyzed the photobleaching profile of several GFP versions, engineered to self-assemble as tumour-homing nanoparticles with different targeting, size and structural stability. This has been done under prolonged irradiation in confocal laser scanning microscopy and by small-angle X-ray scattering. The results show that the oligomerization of GFP at the nanoscale enhances, by more than seven-fold, the stability of fluorescence emission. Interestingly, GFP nanoparticles are much more resistant to X-ray damage than the building block counterparts, indicating that the gained photostability is linked to enhanced structural resistance to radiation. Therefore, the controlled oligomerization of self-assembling fluorescent proteins as protein nanoparticles is a simple, versatile and powerful method to enhance their photostability for uses in precision imaging and therapy. Statement of significance Fluorescent protein assembly into regular and highly symmetric nanoscale structures has been identified to confer enhanced structural stability against radiation stresses dramatically reducing their photobleaching. Being this the main bottleneck in the use of fluorescent proteins for imaging and theranostics, this protein architecture engineering principle appears as a powerful method to enhance their photostability for a broad applicability in precision imaging, drug delivery and theranostics.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Unzueta U. et al. Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP. // Acta Biomaterialia. 2020. Vol. 103. pp. 272-280.
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Unzueta U., Roldan M., Pesarrodona M., Benitez R., Sánchez-Chardi A., Conchillo Solé O., Mangues R., Villaverde A., Vazquez E. Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP. // Acta Biomaterialia. 2020. Vol. 103. pp. 272-280.
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TY - JOUR
DO - 10.1016/j.actbio.2019.12.003
UR - https://doi.org/10.1016%2Fj.actbio.2019.12.003
TI - Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP.
T2 - Acta Biomaterialia
AU - Unzueta, U.
AU - Roldan, Mònica
AU - Pesarrodona, Mireia
AU - Benitez, Raul
AU - Sánchez-Chardi, Alejandro
AU - Conchillo Solé, Oscar
AU - Mangues, Ramon
AU - Villaverde, A.
AU - Vazquez, Ester
PY - 2020
DA - 2020/02/01 00:00:00
PB - Elsevier
SP - 272-280
VL - 103
SN - 1742-7061
ER -
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@article{2020_Unzueta,
author = {U. Unzueta and Mònica Roldan and Mireia Pesarrodona and Raul Benitez and Alejandro Sánchez-Chardi and Oscar Conchillo Solé and Ramon Mangues and A. Villaverde and Ester Vazquez},
title = {Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumor-targeted GFP.},
journal = {Acta Biomaterialia},
year = {2020},
volume = {103},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1016%2Fj.actbio.2019.12.003},
pages = {272--280},
doi = {10.1016/j.actbio.2019.12.003}
}