Periodical amphiphilic surface with chemical patterning for micelles immobilization and analysis

• Preparation of periodically patterned surface with the controlled hydrophilic and hydrophobic nanoareas is presented. • Proposed approach comprises advanced colloid lithography and diazonium-chemistry based grafting. • Proposed procedure represents a versatile and scalable method for the creation of stable amphiphilic coating. • Prepared surfaces were applied for immobilization and detection of drug-loaded micelles. We present a strategy to produce periodically patterned surface with the controlled hydrophilic and hydrophobic nanoareas. The developed approach comprises colloid lithography using vapor-annealed polystyrene (PS) microspheres mask and two-step electrochemical surface grafting. The grafting was performed using the diazonium surface chemistry before and after colloid mask removal in order to introduce hydrophilic Ph-COOH and hydrophobic Ph-C 8 F 17 moieties. Grafted chemical moities were covalently attached to the surface. The procedure results in creation of a surface with nanoprecisely located periodical hydrophobic and hydrophilic areas, with different sizes and geometry, determined by vapor annealing of PS microspheres. The created amphiphilic surface shows good biorepelency towards bovine serum albumin (BSA) and a high affinity towards amphiphilic micelles. Variation of surface nanoarchitecture elucidates the optimal surface chemistry for immobilization and surface entrapping of drug-loaded micelles. The formation of additional interface between amphilic surface and micelles was proved by dramatic decrease of the contact angle. According to Fourier-transform infrared spectroscopy (FTIR) results, created amphiphilic surfaces can capture drug-loaded micelles even in the presence of BSA biomatrix. Described procedure represents a versatile and scalable method for the creation of stable amphiphilic coating and its potential application in qualitative analysis and identification of micelles.