Open Access
Cell, volume 150, issue 3, pages 481-494
TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends
Zhong Franklin
1
,
Batista Luis F Z
1
,
Freund Adam
2
,
Pech Matthew F
2
,
Venteicher Andrew
2
,
Artandi Steven E.
2
1
Department of Medicine, Stanford University School of Medicine Stanford, CA 94305 USA
|
2
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
|
Publication type: Journal Article
Publication date: 2012-08-02
PubMed ID:
22863003
General Biochemistry, Genetics and Molecular Biology
Abstract
Telomere synthesis in cancer cells and stem cells involves trafficking of telomerase to Cajal bodies, and telomerase is thought to be recruited to telomeres through interactions with telomere-binding proteins. Here, we show that the OB-fold domain of the telomere-binding protein TPP1 recruits telomerase to telomeres through an association with the telomerase reverse transcriptase TERT. When tethered away from telomeres and other telomere-binding proteins, the TPP1 OB-fold domain is sufficient to recruit telomerase to a heterologous chromatin locus. Expression of a minimal TPP1 OB-fold inhibits telomere maintenance by blocking access of telomerase to its cognate binding site at telomeres. We identify amino acids required for the TPP1-telomerase interaction, including specific loop residues within the TPP1 OB-fold domain and individual residues within TERT, some of which are mutated in a subset of pulmonary fibrosis patients. These data define a potential interface for telomerase-TPP1 interaction required for telomere maintenance and implicate defective telomerase recruitment in telomerase-related disease.
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Citations by publishers
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zhong F. et al. TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends // Cell. 2012. Vol. 150. No. 3. pp. 481-494.
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Zhong F., Batista L. F. Z., Freund A., Pech M. F., Venteicher A., Artandi S. E. TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends // Cell. 2012. Vol. 150. No. 3. pp. 481-494.
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TY - JOUR
DO - 10.1016/j.cell.2012.07.012
UR - https://doi.org/10.1016%2Fj.cell.2012.07.012
TI - TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends
T2 - Cell
AU - Zhong, Franklin
AU - Batista, Luis F Z
AU - Freund, Adam
AU - Pech, Matthew F
AU - Venteicher, Andrew
AU - Artandi, Steven E.
PY - 2012
DA - 2012/08/02 00:00:00
PB - Elsevier
SP - 481-494
IS - 3
VL - 150
PMID - 22863003
SN - 0092-8674
SN - 1097-4172
ER -
Cite this
BibTex
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@article{2012_Zhong,
author = {Franklin Zhong and Luis F Z Batista and Adam Freund and Matthew F Pech and Andrew Venteicher and Steven E. Artandi},
title = {TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends},
journal = {Cell},
year = {2012},
volume = {150},
publisher = {Elsevier},
month = {aug},
url = {https://doi.org/10.1016%2Fj.cell.2012.07.012},
number = {3},
pages = {481--494},
doi = {10.1016/j.cell.2012.07.012}
}
Cite this
MLA
Copy
Zhong, Franklin, et al. “TPP1 OB-Fold Domain Controls Telomere Maintenance by Recruiting Telomerase to Chromosome Ends.” Cell, vol. 150, no. 3, Aug. 2012, pp. 481-494. https://doi.org/10.1016%2Fj.cell.2012.07.012.