Open Access
Cell, volume 153, issue 6, pages 1340-1353
Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions
Publication type: Journal Article
Publication date: 2013-06-06
General Biochemistry, Genetics and Molecular Biology
Abstract
Yeast telomeres comprise irregular TG₁₋₃ DNA repeats bound by the general transcription factor Rap1. Rif1 and Rif2, along with Rap1, form the telosome, a protective cap that inhibits telomerase, counteracts SIR-mediated transcriptional silencing, and prevents inadvertent recognition of telomeres as DNA double-strand breaks. We provide a molecular, biochemical, and functional dissection of the protein backbone at the core of the yeast telosome. The X-ray structures of Rif1 and Rif2 bound to the Rap1 C-terminal domain and that of the Rif1 C terminus are presented. Both Rif1 and Rif2 have separable and independent Rap1-binding epitopes, allowing Rap1 binding over large distances (42-110 Å). We identify tetramerization (Rif1) and polymerization (Rif2) modules that, in conjunction with the long-range binding, give rise to a higher-order architecture that interlinks Rap1 units. This molecular Velcro relies on Rif1 and Rif2 to recruit and stabilize Rap1 on telomeric arrays and is required for telomere homeostasis in vivo.
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Citations by publishers
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shi T. et al. Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions // Cell. 2013. Vol. 153. No. 6. pp. 1340-1353.
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Shi T., Bunker R. D., Mattarocci S., Ribeyre C., FATY M., Gut H., Scrima A., Rass U., Rubin S. M., Shore D., Thomä N. H. Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions // Cell. 2013. Vol. 153. No. 6. pp. 1340-1353.
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TY - JOUR
DO - 10.1016/j.cell.2013.05.007
UR - https://doi.org/10.1016%2Fj.cell.2013.05.007
TI - Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions
T2 - Cell
AU - Shi, Tianlai
AU - Bunker, Richard D.
AU - Mattarocci, Stefano
AU - Ribeyre, Cyril
AU - FATY, Mahamadou
AU - Gut, Heinz
AU - Scrima, Andrea
AU - Rass, Ulrich
AU - Rubin, Seth M
AU - Shore, David
AU - Thomä, Nicolas H.
PY - 2013
DA - 2013/06/06 00:00:00
PB - Elsevier
SP - 1340-1353
IS - 6
VL - 153
SN - 0092-8674
SN - 1097-4172
ER -
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@article{2013_Shi,
author = {Tianlai Shi and Richard D. Bunker and Stefano Mattarocci and Cyril Ribeyre and Mahamadou FATY and Heinz Gut and Andrea Scrima and Ulrich Rass and Seth M Rubin and David Shore and Nicolas H. Thomä},
title = {Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions},
journal = {Cell},
year = {2013},
volume = {153},
publisher = {Elsevier},
month = {jun},
url = {https://doi.org/10.1016%2Fj.cell.2013.05.007},
number = {6},
pages = {1340--1353},
doi = {10.1016/j.cell.2013.05.007}
}
Cite this
MLA
Copy
Shi, Tianlai, et al. “Rif1 and Rif2 Shape Telomere Function and Architecture through Multivalent Rap1 Interactions.” Cell, vol. 153, no. 6, Jun. 2013, pp. 1340-1353. https://doi.org/10.1016%2Fj.cell.2013.05.007.