Open Access
Cell, volume 155, issue 2, pages 345-356
Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation
Publication type: Journal Article
Publication date: 2013-10-10
PubMed ID:
24120135
General Biochemistry, Genetics and Molecular Biology
Abstract
We have applied a super-resolution fluorescence imaging method, stochastic optical reconstruction microscopy (STORM), to visualize the structure of functional telomeres and telomeres rendered dysfunctional through removal of shelterin proteins. The STORM images showed that functional telomeres frequently exhibit a t-loop configuration. Conditional deletion of individual components of shelterin showed that TRF2 was required for the formation and/or maintenance of t-loops, whereas deletion of TRF1, Rap1, or the POT1 proteins (POT1a and POT1b) had no effect on the frequency of t-loop occurrence. Within the shelterin complex, TRF2 uniquely serves to protect telomeres from two pathways that are initiated on free DNA ends: classical nonhomologous end-joining (NHEJ) and ATM-dependent DNA damage signaling. The TRF2-dependent remodeling of telomeres into t-loop structures, which sequester the ends of chromosomes, can explain why NHEJ and the ATM signaling pathway are repressed when TRF2 is present.
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Doksani Y. et al. Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation // Cell. 2013. Vol. 155. No. 2. pp. 345-356.
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Doksani Y., Wu J. Y., de Lange T., ZHUANG X. Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation // Cell. 2013. Vol. 155. No. 2. pp. 345-356.
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TY - JOUR
DO - 10.1016/j.cell.2013.09.048
UR - https://doi.org/10.1016%2Fj.cell.2013.09.048
TI - Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation
T2 - Cell
AU - Doksani, Ylli
AU - Wu, John Y
AU - de Lange, Titia
AU - ZHUANG, XIAOWEI
PY - 2013
DA - 2013/10/10 00:00:00
PB - Elsevier
SP - 345-356
IS - 2
VL - 155
PMID - 24120135
SN - 0092-8674
SN - 1097-4172
ER -
Cite this
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@article{2013_Doksani,
author = {Ylli Doksani and John Y Wu and Titia de Lange and XIAOWEI ZHUANG},
title = {Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation},
journal = {Cell},
year = {2013},
volume = {155},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016%2Fj.cell.2013.09.048},
number = {2},
pages = {345--356},
doi = {10.1016/j.cell.2013.09.048}
}
Cite this
MLA
Copy
Doksani, Ylli, et al. “Super-Resolution Fluorescence Imaging of Telomeres Reveals TRF2-Dependent T-loop Formation.” Cell, vol. 155, no. 2, Oct. 2013, pp. 345-356. https://doi.org/10.1016%2Fj.cell.2013.09.048.