Cell, volume 172, issue 1-2, pages 331-343.e13
Structural Insights into Yeast Telomerase Recruitment to Telomeres
Chen Hongwen
1
,
Jing Xue
1
,
Churikov Dmitri
2
,
Hass Evan P
3
,
SHI SHAOHUA
1
,
Lemon Laramie D
4
,
Luciano Pierre
2
,
Bertuch Alison
4
,
Zappulla David C
3
,
Géli Vincent
2
,
Wu Jian
1
,
Lei Ming
5
4
Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, BCM225, Houston, TX 77030, USA.
|
Publication type: Journal Article
Publication date: 2018-01-01
PubMed ID:
29290466
General Biochemistry, Genetics and Molecular Biology
Abstract
Telomerase maintains chromosome ends from humans to yeasts. Recruitment of yeast telomerase to telomeres occurs through its Ku and Est1 subunits via independent interactions with telomerase RNA (TLC1) and telomeric proteins Sir4 and Cdc13, respectively. However, the structures of the molecules comprising these telomerase-recruiting pathways remain unknown. Here, we report crystal structures of the Ku heterodimer and Est1 complexed with their key binding partners. Two major findings are as follows: (1) Ku specifically binds to telomerase RNA in a distinct, yet related, manner to how it binds DNA; and (2) Est1 employs two separate pockets to bind distinct motifs of Cdc13. The N-terminal Cdc13-binding site of Est1 cooperates with the TLC1-Ku-Sir4 pathway for telomerase recruitment, whereas the C-terminal interface is dispensable for binding Est1 in vitro yet is nevertheless essential for telomere maintenance in vivo. Overall, our results integrate previous models and provide fundamentally valuable structural information regarding telomere biology.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Chen H. et al. Structural Insights into Yeast Telomerase Recruitment to Telomeres // Cell. 2018. Vol. 172. No. 1-2. p. 331-343.e13.
GOST all authors (up to 50)
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Chen H., Jing X., Churikov D., Hass E. P., SHI S., Lemon L. D., Luciano P., Bertuch A., Zappulla D. C., Géli V., Wu J., Lei M. Structural Insights into Yeast Telomerase Recruitment to Telomeres // Cell. 2018. Vol. 172. No. 1-2. p. 331-343.e13.
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RIS
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TY - JOUR
DO - 10.1016/j.cell.2017.12.008
UR - https://doi.org/10.1016%2Fj.cell.2017.12.008
TI - Structural Insights into Yeast Telomerase Recruitment to Telomeres
T2 - Cell
AU - Chen, Hongwen
AU - Jing, Xue
AU - Churikov, Dmitri
AU - Hass, Evan P
AU - SHI, SHAOHUA
AU - Lemon, Laramie D
AU - Luciano, Pierre
AU - Bertuch, Alison
AU - Zappulla, David C
AU - Géli, Vincent
AU - Wu, Jian
AU - Lei, Ming
PY - 2018
DA - 2018/01/01 00:00:00
PB - Elsevier
SP - 331-343.e13
IS - 1-2
VL - 172
PMID - 29290466
SN - 0092-8674
SN - 1097-4172
ER -
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@article{2018_Chen,
author = {Hongwen Chen and Xue Jing and Dmitri Churikov and Evan P Hass and SHAOHUA SHI and Laramie D Lemon and Pierre Luciano and Alison Bertuch and David C Zappulla and Vincent Géli and Jian Wu and Ming Lei},
title = {Structural Insights into Yeast Telomerase Recruitment to Telomeres},
journal = {Cell},
year = {2018},
volume = {172},
publisher = {Elsevier},
month = {jan},
url = {https://doi.org/10.1016%2Fj.cell.2017.12.008},
number = {1-2},
pages = {331--343.e13},
doi = {10.1016/j.cell.2017.12.008}
}
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MLA
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Chen, Hongwen, et al. “Structural Insights into Yeast Telomerase Recruitment to Telomeres.” Cell, vol. 172, no. 1-2, Jan. 2018, pp. 331-343.e13. https://doi.org/10.1016%2Fj.cell.2017.12.008.