Open Access
Cell Reports, volume 9, issue 4, pages 1273-1280
TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation
Publication type: Journal Article
Publication date: 2014-11-06
PubMed ID:
25453752
General Biochemistry, Genetics and Molecular Biology
Abstract
The conserved protein Rap1 functions at telomeres in fungi, protozoa, and vertebrates. Like yeast Rap1, human Rap1 has been implicated in telomere length regulation and repression of nonhomologous end-joining (NHEJ) at telomeres. However, mouse telomeres lacking Rap1 do not succumb to NHEJ. To determine the functions of human Rap1, we generated several transcription activator-like effector nuclease (TALEN)-mediated human cell lines lacking Rap1. Loss of Rap1 did not affect the other components of shelterin, the modification of telomeric histones, the subnuclear position of telomeres, or the 3' telomeric overhang. Telomeres lacking Rap1 did not show a DNA damage response, NHEJ, or consistent changes in their length, indicating that Rap1 does not have an important function in protection or length regulation of human telomeres. As human Rap1, like its mouse and unicellular orthologs, affects gene expression, we propose that the conservation of Rap1 reflects its role in transcriptional regulation rather than a function at telomeres.
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Kabir S., Hockemeyer D., de Lange T. TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation // Cell Reports. 2014. Vol. 9. No. 4. pp. 1273-1280.
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Kabir S., Hockemeyer D., de Lange T. TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation // Cell Reports. 2014. Vol. 9. No. 4. pp. 1273-1280.
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TY - JOUR
DO - 10.1016/j.celrep.2014.10.014
UR - https://doi.org/10.1016%2Fj.celrep.2014.10.014
TI - TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation
T2 - Cell Reports
AU - Kabir, Shaheen
AU - Hockemeyer, Dirk
AU - de Lange, Titia
PY - 2014
DA - 2014/11/06 00:00:00
PB - Elsevier
SP - 1273-1280
IS - 4
VL - 9
PMID - 25453752
SN - 2211-1247
ER -
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@article{2014_Kabir,
author = {Shaheen Kabir and Dirk Hockemeyer and Titia de Lange},
title = {TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation},
journal = {Cell Reports},
year = {2014},
volume = {9},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016%2Fj.celrep.2014.10.014},
number = {4},
pages = {1273--1280},
doi = {10.1016/j.celrep.2014.10.014}
}
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MLA
Copy
Kabir, Shaheen, et al. “TALEN Gene Knockouts Reveal No Requirement for the Conserved Human Shelterin Protein Rap1 in Telomere Protection and Length Regulation.” Cell Reports, vol. 9, no. 4, Nov. 2014, pp. 1273-1280. https://doi.org/10.1016%2Fj.celrep.2014.10.014.