Open Access
Cell Reports, volume 33, issue 12, pages 108527
An RNA Repair Operon Regulated by Damaged tRNAs
1
Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA
|
2
RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
|
3
Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
|
Publication type: Journal Article
Publication date: 2020-12-22
PubMed ID:
33357439
General Biochemistry, Genetics and Molecular Biology
Abstract
Many bacteria contain an RNA repair operon, encoding the RtcB RNA ligase and the RtcA RNA cyclase, that is regulated by the RtcR transcriptional activator. Although RtcR contains a divergent version of the CARF (CRISPR-associated Rossman fold) oligonucleotide-binding regulatory domain, both the specific signal that regulates operon expression and the substrates of the encoded enzymes are unknown. We report that tRNA fragments activate operon expression. Using a genetic screen in Salmonella enterica serovar Typhimurium, we find that the operon is expressed in the presence of mutations that cause tRNA fragments to accumulate. RtcA, which converts RNA phosphate ends to 2', 3'-cyclic phosphate, is also required. Operon expression and tRNA fragment accumulation also occur upon DNA damage. The CARF domain binds 5' tRNA fragments ending in cyclic phosphate, and RtcR oligomerizes upon binding these ligands, a prerequisite for operon activation. Our studies reveal a signaling pathway involving broken tRNAs and implicate the operon in tRNA repair.
Citations by journals
|
1
2
|
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
2 publications, 6.9%
|
|
Journal of Bacteriology
|
Journal of Bacteriology
2 publications, 6.9%
|
|
Genes
|
Genes
1 publication, 3.45%
|
|
Viruses
|
Viruses
1 publication, 3.45%
|
|
Frontiers in Molecular Biosciences
|
Frontiers in Molecular Biosciences
1 publication, 3.45%
|
|
RNA Biology
|
RNA Biology
1 publication, 3.45%
|
|
Molecular Cell
|
Molecular Cell
1 publication, 3.45%
|
|
Cell
|
Cell
1 publication, 3.45%
|
|
iScience
|
iScience
1 publication, 3.45%
|
|
mBio
|
mBio
1 publication, 3.45%
|
|
RNA
|
RNA
1 publication, 3.45%
|
|
Nucleic Acids Research
|
Nucleic Acids Research
1 publication, 3.45%
|
|
Communications Biology
|
Communications Biology
1 publication, 3.45%
|
|
Journal of Biological Chemistry
|
Journal of Biological Chemistry
1 publication, 3.45%
|
|
Chemical Communications
|
Chemical Communications
1 publication, 3.45%
|
|
Critical Reviews in Biochemistry and Molecular Biology
|
Critical Reviews in Biochemistry and Molecular Biology
1 publication, 3.45%
|
|
Annual Review of Genetics
|
Annual Review of Genetics
1 publication, 3.45%
|
|
Cell Chemical Biology
|
Cell Chemical Biology
1 publication, 3.45%
|
|
Current Opinion in Microbiology
|
Current Opinion in Microbiology
1 publication, 3.45%
|
|
1
2
|
Citations by publishers
|
1
2
3
4
5
|
|
|
Elsevier
|
Elsevier
5 publications, 17.24%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 13.79%
|
|
American Society for Microbiology
|
American Society for Microbiology
3 publications, 10.34%
|
|
Taylor & Francis
|
Taylor & Francis
2 publications, 6.9%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
1 publication, 3.45%
|
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
1 publication, 3.45%
|
|
Oxford University Press
|
Oxford University Press
1 publication, 3.45%
|
|
Springer Nature
|
Springer Nature
1 publication, 3.45%
|
|
American Society for Biochemistry and Molecular Biology
|
American Society for Biochemistry and Molecular Biology
1 publication, 3.45%
|
|
Royal Society of Chemistry (RSC)
|
Royal Society of Chemistry (RSC)
1 publication, 3.45%
|
|
Annual Reviews
|
Annual Reviews
1 publication, 3.45%
|
|
1
2
3
4
5
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[7,10,10,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["24.14","34.48","34.48","6.9"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","Journal of Bacteriology","Genes","Viruses","Frontiers in Molecular Biosciences","RNA Biology","Molecular Cell","Cell","iScience","mBio","RNA","Nucleic Acids Research","Communications Biology","Journal of Biological Chemistry","Chemical Communications","Critical Reviews in Biochemistry and Molecular Biology","Annual Review of Genetics","Cell Chemical Biology","Current Opinion in Microbiology"],"ids":[14627,21892,8879,2455,3133,7613,19820,16552,6073,9121,12892,23904,11778,19451,9073,19938,22544,4893,16284],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[6.9,6.9,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45,3.45],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","American Society for Microbiology","Taylor & Francis","Frontiers Media S.A.","Cold Spring Harbor Laboratory","Oxford University Press","Springer Nature","American Society for Biochemistry and Molecular Biology","Royal Society of Chemistry (RSC)","Annual Reviews"],"ids":[17,202,142,18,208,6909,19,8,2090,123,6941],"codes":[0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/leiAYcRDGTSl5B1eCnwpSGqmDEUEfDPPoYisFGhT_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp"],"datasets":[{"label":"","data":[5,4,3,2,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[17.24,13.79,10.34,6.9,3.45,3.45,3.45,3.45,3.45,3.45,3.45],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Hughes K. et al. An RNA Repair Operon Regulated by Damaged tRNAs // Cell Reports. 2020. Vol. 33. No. 12. p. 108527.
GOST all authors (up to 50)
Copy
Hughes K., Chen X., Burroughs A. M., Aravind L., Wolin S. L. An RNA Repair Operon Regulated by Damaged tRNAs // Cell Reports. 2020. Vol. 33. No. 12. p. 108527.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1016/j.celrep.2020.108527
UR - https://doi.org/10.1016%2Fj.celrep.2020.108527
TI - An RNA Repair Operon Regulated by Damaged tRNAs
T2 - Cell Reports
AU - Hughes, Kevin
AU - Chen, Xinguo
AU - Burroughs, A Maxwell
AU - Aravind, L
AU - Wolin, Sandra L
PY - 2020
DA - 2020/12/22 00:00:00
PB - Elsevier
SP - 108527
IS - 12
VL - 33
PMID - 33357439
SN - 2211-1247
ER -
Cite this
BibTex
Copy
@article{2020_Hughes,
author = {Kevin Hughes and Xinguo Chen and A Maxwell Burroughs and L Aravind and Sandra L Wolin},
title = {An RNA Repair Operon Regulated by Damaged tRNAs},
journal = {Cell Reports},
year = {2020},
volume = {33},
publisher = {Elsevier},
month = {dec},
url = {https://doi.org/10.1016%2Fj.celrep.2020.108527},
number = {12},
pages = {108527},
doi = {10.1016/j.celrep.2020.108527}
}
Cite this
MLA
Copy
Hughes, Kevin, et al. “An RNA Repair Operon Regulated by Damaged tRNAs.” Cell Reports, vol. 33, no. 12, Dec. 2020, p. 108527. https://doi.org/10.1016%2Fj.celrep.2020.108527.