Colloids and Surfaces B: Biointerfaces, volume 176, pages 256-264
Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay
Kropaneva Maria
1
,
Timganova Valeria
,
Zamorina Svetlana
,
Minin A.S.
2, 3
,
Bochkova Maria
,
Rayev Mikhail
,
Yermakov Anatoly
,
Uimin Mikhail
,
Byzov Ilya
3
,
Mysik Alexey
4
,
Timganova V. P.
1
,
Bochkova M. S.
1
,
Uimin M.A.
2, 3
,
Zamorina S. A.
1
,
Yermakov A.Y.
2, 3
,
Rayev M. B.
1, 2
Publication type: Journal Article
Publication date: 2019-04-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.8
ISSN: 09277765, 18734367
Physical and Theoretical Chemistry
General Medicine
Colloid and Surface Chemistry
Biotechnology
Surfaces and Interfaces
Abstract
In this work, we developed and optimized conjugates of carbon-coated iron nanoparticles (Fe@C) with streptavidin and monoclonal antibodies. The conjugation procedure included two stages. First, amino groups were grafted onto the carbon shell to facilitate noncovalent sorption of bovine serum albumin (BSA). Further, the covalent attachment of proteins to the BSA layer via glutaraldehyde coupling was performed. It was established and confirmed that the synthesis procedure is reproducible and allows preparation of stable conjugates. The resulting nanoparticles are clusters of Fe@C particles coated by proteins. The size of the clusters is in the range of 100-190 nm and can be controlled via the tuning of conjugation conditions, including pH, BSA-to-Fe@C ratio, etc. Conjugates of Fe@C with streptavidin and monoclonal antibodies (sizes of approximately 140-150 nm) were synthesized. Proton T2 relaxometry was used to detect these conjugates with very high sensitivity due to the magnetic markers, Fe@C. The relaxivity (r2) of different conjugates varied within the range of 290-450 1/s*mM. Conjugate applicability for relaxometry-based assay was confirmed by direct detection of streptococcal protein G and biotinylated BSA in a dot immunoassay.
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Khramtsov P. V. et al. Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay // Colloids and Surfaces B: Biointerfaces. 2019. Vol. 176. pp. 256-264.
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Kropaneva M., Rayev M., Zamorina S., Timganova V., Yermakov A., Uimin M., Bochkova M., Mysik A., Minin A., Byzov I., Khramtsov P. V., Timganova V. P., Bochkova M. S., Uimin M., Zamorina S. A., Yermakov A., Rayev M. B. Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay // Colloids and Surfaces B: Biointerfaces. 2019. Vol. 176. pp. 256-264.
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TY - JOUR
DO - 10.1016/j.colsurfb.2019.01.009
UR - https://doi.org/10.1016%2Fj.colsurfb.2019.01.009
TI - Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay
T2 - Colloids and Surfaces B: Biointerfaces
AU - Khramtsov, Pavel V
AU - Kropaneva, Maria
AU - Byzov, Ilya
AU - Minin, A.S.
AU - Mysik, Alexey
AU - Bochkova, Maria
AU - Uimin, Mikhail
AU - Yermakov, Anatoly
AU - Timganova, Valeria
AU - Zamorina, Svetlana
AU - Rayev, Mikhail
AU - Timganova, V. P.
AU - Bochkova, M. S.
AU - Uimin, M.A.
AU - Zamorina, S. A.
AU - Yermakov, A.Y.
AU - Rayev, M. B.
PY - 2019
DA - 2019/04/01 00:00:00
PB - Elsevier
SP - 256-264
VL - 176
SN - 0927-7765
SN - 1873-4367
ER -
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@article{2019_Khramtsov
author = {Pavel V Khramtsov and Maria Kropaneva and Ilya Byzov and A.S. Minin and Alexey Mysik and Maria Bochkova and Mikhail Uimin and Anatoly Yermakov and Valeria Timganova and Svetlana Zamorina and Mikhail Rayev and V. P. Timganova and M. S. Bochkova and M.A. Uimin and S. A. Zamorina and A.Y. Yermakov and M. B. Rayev},
title = {Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay},
journal = {Colloids and Surfaces B: Biointerfaces},
year = {2019},
volume = {176},
publisher = {Elsevier},
month = {apr},
url = {https://doi.org/10.1016%2Fj.colsurfb.2019.01.009},
pages = {256--264},
doi = {10.1016/j.colsurfb.2019.01.009}
}