Open Access
Open access
Journal of Biological Chemistry, volume 298, issue 5, pages 101914

Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu

Shulenina Olga 3, 4
Treshin Vsevolod 2
Subach Maksim F 2
Serebryakova Marina V. 5, 6
Osterman Ilya A. 2, 5
Paleskava Alena 4, 7
Bogdanov Alexey A. 2, 6
Dontsova Olga A. 1, 2, 5, 6
Konevega Andrey L. 3, 4, 7
Sergiev Petr V. 2, 5, 6, 8
Publication typeJournal Article
Publication date2022-05-01
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.8
ISSN00219258, 1083351X
Biochemistry
Molecular Biology
Cell Biology
Abstract

N-terminal acetylation is widespread in the eukaryotic proteome but in bacteria is restricted to a small number of proteins mainly involved in translation. It was long known that elongation factor Tu (EF-Tu) is N-terminally acetylated, whereas the enzyme responsible for this process was unclear. Here, we report that RimI acetyltransferase, known to modify ribosomal protein S18, is likewise responsible for N-acetylation of the EF-Tu. With the help of inducible tufA expression plasmid, we demonstrated that the acetylation does not alter the stability of EF-Tu. Binding of aminoacyl tRNA to the recombinant EF-Tu in vitro was found to be unaffected by the acetylation. At the same time, with the help of fast kinetics methods, we demonstrate that an acetylated variant of EF-Tu more efficiently accelerates A-site occupation by aminoacyl-tRNA, thus increasing the efficiency of in vitro translation. Finally, we show that a strain devoid of RimI has a reduced growth rate, expanded to an evolutionary timescale, and might potentially promote conservation of the acetylation mechanism of S18 and EF-Tu. This study increased our understanding of the modification of bacterial translation apparatus.

Citations by journals

1
2
Nucleic Acids Research
Nucleic Acids Research, 2, 18.18%
Nucleic Acids Research
2 publications, 18.18%
Antioxidants
Antioxidants, 1, 9.09%
Antioxidants
1 publication, 9.09%
Genomics, Proteomics and Bioinformatics
Genomics, Proteomics and Bioinformatics, 1, 9.09%
Genomics, Proteomics and Bioinformatics
1 publication, 9.09%
Journal of Experimental Botany
Journal of Experimental Botany, 1, 9.09%
Journal of Experimental Botany
1 publication, 9.09%
Biomolecules
Biomolecules, 1, 9.09%
Biomolecules
1 publication, 9.09%
PLoS ONE
PLoS ONE, 1, 9.09%
PLoS ONE
1 publication, 9.09%
Scientific Reports
Scientific Reports, 1, 9.09%
Scientific Reports
1 publication, 9.09%
1
2

Citations by publishers

1
2
3
Oxford University Press
Oxford University Press, 3, 27.27%
Oxford University Press
3 publications, 27.27%
Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 2, 18.18%
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 18.18%
Elsevier
Elsevier, 1, 9.09%
Elsevier
1 publication, 9.09%
Public Library of Science (PLoS)
Public Library of Science (PLoS), 1, 9.09%
Public Library of Science (PLoS)
1 publication, 9.09%
Springer Nature
Springer Nature, 1, 9.09%
Springer Nature
1 publication, 9.09%
1
2
3
  • We do not take into account publications that without a DOI.
  • Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
  • Statistics recalculated weekly.
Metrics
Share
Cite this
GOST |
Cite this
GOST Copy
Pletnev P. I. et al. Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu // Journal of Biological Chemistry. 2022. Vol. 298. No. 5. p. 101914.
GOST all authors (up to 50) Copy
Pletnev P. I., Shulenina O., Evfratov S., Treshin V., Subach M. F., Serebryakova M., Osterman I. A., Paleskava A., Bogdanov A. A., Dontsova O. A., Konevega A. L., Sergiev P. V. Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu // Journal of Biological Chemistry. 2022. Vol. 298. No. 5. p. 101914.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1016/j.jbc.2022.101914
UR - https://doi.org/10.1016%2Fj.jbc.2022.101914
TI - Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu
T2 - Journal of Biological Chemistry
AU - Pletnev, Philipp I
AU - Shulenina, Olga
AU - Evfratov, Sergey
AU - Treshin, Vsevolod
AU - Subach, Maksim F
AU - Serebryakova, Marina V.
AU - Osterman, Ilya A.
AU - Paleskava, Alena
AU - Bogdanov, Alexey A.
AU - Dontsova, Olga A.
AU - Konevega, Andrey L.
AU - Sergiev, Petr V.
PY - 2022
DA - 2022/05/01 00:00:00
PB - American Society for Biochemistry and Molecular Biology
SP - 101914
IS - 5
VL - 298
PMID - 35398352
SN - 0021-9258
SN - 1083-351X
ER -
BibTex |
Cite this
BibTex Copy
@article{2022_Pletnev
author = {Philipp I Pletnev and Olga Shulenina and Sergey Evfratov and Vsevolod Treshin and Maksim F Subach and Marina V. Serebryakova and Ilya A. Osterman and Alena Paleskava and Alexey A. Bogdanov and Olga A. Dontsova and Andrey L. Konevega and Petr V. Sergiev},
title = {Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu},
journal = {Journal of Biological Chemistry},
year = {2022},
volume = {298},
publisher = {American Society for Biochemistry and Molecular Biology},
month = {may},
url = {https://doi.org/10.1016%2Fj.jbc.2022.101914},
number = {5},
pages = {101914},
doi = {10.1016/j.jbc.2022.101914}
}
MLA
Cite this
MLA Copy
Pletnev, Philipp I., et al. “Ribosomal protein S18 acetyltransferase RimI is responsible for the acetylation of elongation factor Tu.” Journal of Biological Chemistry, vol. 298, no. 5, May. 2022, p. 101914. https://doi.org/10.1016%2Fj.jbc.2022.101914.
Found error?