Journal of Biotechnology, volume 124, issue 2, pages 469-472

High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow

Salamone Daniel Felipe ¹

Barañao Lino ²

Santos Cláudio ³

Bussmann Leonardo ⁴

Artuso Jorge ³

Werning Carlos ³

Prync Aida ³

Carbonetto Cesar ³

Dabsys Susana ³

Munar Carlos ⁵

Salaberry Roberto ³

Berra Guillermo ⁶

Berra Ignacio ³

Fernández Nahuel ³

Papouchado Mariana ³

Foti Marcelo ³

Judewicz Norberto ³

Mujica Ignacio ⁵

Munoz Luciana ³

Alvarez Silvina Fenández ³

González Eliseo ⁷

Zimmermann Juan ³

Criscuolo Marcelo ³

Melo Carlos Eduardo ³

Hide authors affiliations Show authors affiliations: 7 affiliations

Biosidus SA, Buenos Aires, Argentina. |

Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina |

BioSidus S.A., Buenos Aires, Argentina |

⁴

Instituto de Biología y Medicina Experimental-CONICET, Argentina. |

⁵

Munar y Asociados, La Plata, Pcia de Buenos Aires, Buenos Aires, Argentina |

⁶

Instituto Nacional de Tecnología Agropecuaria, Argentina |

⁷

Centro de Diagnóstico Molecular S.A., Buenos Aires, Argentina |

Publication type: Journal Article

Publication date: 2006-07-01

Elsevier

Journal: Journal of Biotechnology

Quartile SCImago

Quartile WOS

Impact factor: 4.1

ISSN: 01681656, 18734863

DOI: 10.1016/j.jbiotec.2006.01.005

Copy DOI

General Medicine

Applied Microbiology and Biotechnology

Biotechnology

Bioengineering

Abstract

Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.

By date By citations

Citations by journals

	1 2 3 4 5
Transgenic Research	Transgenic Research, 5, 9.26% Transgenic Research 5 publications, 9.26%
Zygote	Zygote, 5, 9.26% Zygote 5 publications, 9.26%
Cellular Reprogramming	Cellular Reprogramming, 2, 3.7% Cellular Reprogramming 2 publications, 3.7%
PLoS ONE	PLoS ONE, 2, 3.7% PLoS ONE 2 publications, 3.7%
Animal Reproduction Science	Animal Reproduction Science, 2, 3.7% Animal Reproduction Science 2 publications, 3.7%
Gene	Gene, 2, 3.7% Gene 2 publications, 3.7%
Biotechnology Progress	Biotechnology Progress, 2, 3.7% Biotechnology Progress 2 publications, 3.7%
Reproduction in Domestic Animals	Reproduction in Domestic Animals, 2, 3.7% Reproduction in Domestic Animals 2 publications, 3.7%
Doklady Biochemistry and Biophysics	Doklady Biochemistry and Biophysics, 1, 1.85% Doklady Biochemistry and Biophysics 1 publication, 1.85%
Reproduction, Fertility and Development	Reproduction, Fertility and Development, 1, 1.85% Reproduction, Fertility and Development 1 publication, 1.85%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 1.85% International Journal of Molecular Sciences 1 publication, 1.85%
Chinese Science Bulletin	Chinese Science Bulletin, 1, 1.85% Chinese Science Bulletin 1 publication, 1.85%
Scientific Reports	Scientific Reports, 1, 1.85% Scientific Reports 1 publication, 1.85%
Nature	Nature, 1, 1.85% Nature 1 publication, 1.85%
Journal of Assisted Reproduction and Genetics	Journal of Assisted Reproduction and Genetics, 1, 1.85% Journal of Assisted Reproduction and Genetics 1 publication, 1.85%
Biotechnology Letters	Biotechnology Letters, 1, 1.85% Biotechnology Letters 1 publication, 1.85%
Microbial Cell Factories	Microbial Cell Factories, 1, 1.85% Microbial Cell Factories 1 publication, 1.85%
Revista Argentina de Microbiologia	Revista Argentina de Microbiologia, 1, 1.85% Revista Argentina de Microbiologia 1 publication, 1.85%
Theriogenology	Theriogenology, 1, 1.85% Theriogenology 1 publication, 1.85%
Protein Expression and Purification	Protein Expression and Purification, 1, 1.85% Protein Expression and Purification 1 publication, 1.85%
Biotechnology and Bioengineering	Biotechnology and Bioengineering, 1, 1.85% Biotechnology and Bioengineering 1 publication, 1.85%
Journal of Veterinary Internal Medicine	Journal of Veterinary Internal Medicine, 1, 1.85% Journal of Veterinary Internal Medicine 1 publication, 1.85%
New Zealand Journal of Agricultural Research	New Zealand Journal of Agricultural Research, 1, 1.85% New Zealand Journal of Agricultural Research 1 publication, 1.85%
GM crops & food	GM crops & food, 1, 1.85% GM crops & food 1 publication, 1.85%
Journal of Dairy Science	Journal of Dairy Science, 1, 1.85% Journal of Dairy Science 1 publication, 1.85%
Bioengineered	Bioengineered, 1, 1.85% Bioengineered 1 publication, 1.85%
American Journal of Plant Sciences	American Journal of Plant Sciences, 1, 1.85% American Journal of Plant Sciences 1 publication, 1.85%
Advances in Genetics	Advances in Genetics, 1, 1.85% Advances in Genetics 1 publication, 1.85%
Journal of Reproduction and Development	Journal of Reproduction and Development, 1, 1.85% Journal of Reproduction and Development 1 publication, 1.85%
	1 2 3 4 5

Citations by publishers

	2 4 6 8 10 12
Springer Nature	Springer Nature, 12, 22.22% Springer Nature 12 publications, 22.22%
Elsevier	Elsevier, 10, 18.52% Elsevier 10 publications, 18.52%
Wiley	Wiley, 7, 12.96% Wiley 7 publications, 12.96%
Cambridge University Press	Cambridge University Press, 5, 9.26% Cambridge University Press 5 publications, 9.26%
Taylor & Francis	Taylor & Francis, 3, 5.56% Taylor & Francis 3 publications, 5.56%
Mary Ann Liebert	Mary Ann Liebert, 2, 3.7% Mary Ann Liebert 2 publications, 3.7%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 2, 3.7% Public Library of Science (PLoS) 2 publications, 3.7%
Pleiades Publishing	Pleiades Publishing, 1, 1.85% Pleiades Publishing 1 publication, 1.85%
CSIRO Publishing	CSIRO Publishing, 1, 1.85% CSIRO Publishing 1 publication, 1.85%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 1.85% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 1.85%
Scientific Research Publishing	Scientific Research Publishing, 1, 1.85% Scientific Research Publishing 1 publication, 1.85%
IPEC Inc.	IPEC Inc., 1, 1.85% IPEC Inc. 1 publication, 1.85%
Walter de Gruyter	Walter de Gruyter, 1, 1.85% Walter de Gruyter 1 publication, 1.85%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 1, 1.85% Proceedings of the National Academy of Sciences (PNAS) 1 publication, 1.85%
Higher Education Press	Higher Education Press, 1, 1.85% Higher Education Press 1 publication, 1.85%
Korean Society of Veterinary Science	Korean Society of Veterinary Science, 1, 1.85% Korean Society of Veterinary Science 1 publication, 1.85%
	2 4 6 8 10 12

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
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GOST |

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GOST Copy

Salamone D. F. et al. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow // Journal of Biotechnology. 2006. Vol. 124. No. 2. pp. 469-472.

GOST all authors (up to 50) Copy

Salamone D. F., Barañao L., Santos C., Bussmann L., Artuso J., Werning C., Prync A., Carbonetto C., Dabsys S., Munar C., Salaberry R., Berra G., Berra I., Fernández N., Papouchado M., Foti M., Judewicz N., Mujica I., Munoz L., Alvarez S. F., González E., Zimmermann J., Criscuolo M., Melo C. E. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow // Journal of Biotechnology. 2006. Vol. 124. No. 2. pp. 469-472.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.jbiotec.2006.01.005

UR - https://doi.org/10.1016%2Fj.jbiotec.2006.01.005

TI - High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow

T2 - Journal of Biotechnology

AU - Salamone, Daniel Felipe

AU - Barañao, Lino

AU - Santos, Cláudio

AU - Bussmann, Leonardo

AU - Artuso, Jorge

AU - Werning, Carlos

AU - Prync, Aida

AU - Carbonetto, Cesar

AU - Dabsys, Susana

AU - Munar, Carlos

AU - Salaberry, Roberto

AU - Berra, Guillermo

AU - Berra, Ignacio

AU - Fernández, Nahuel

AU - Papouchado, Mariana

AU - Foti, Marcelo

AU - Judewicz, Norberto

AU - Mujica, Ignacio

AU - Munoz, Luciana

AU - Alvarez, Silvina Fenández

AU - González, Eliseo

AU - Zimmermann, Juan

AU - Criscuolo, Marcelo

AU - Melo, Carlos Eduardo

PY - 2006

DA - 2006/07/01 00:00:00

PB - Elsevier

SP - 469-472

IS - 2

VL - 124

SN - 0168-1656

SN - 1873-4863

ER -

BibTex |

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@article{2006_Salamone,

author = {Daniel Felipe Salamone and Lino Barañao and Cláudio Santos and Leonardo Bussmann and Jorge Artuso and Carlos Werning and Aida Prync and Cesar Carbonetto and Susana Dabsys and Carlos Munar and Roberto Salaberry and Guillermo Berra and Ignacio Berra and Nahuel Fernández and Mariana Papouchado and Marcelo Foti and Norberto Judewicz and Ignacio Mujica and Luciana Munoz and Silvina Fenández Alvarez and Eliseo González and Juan Zimmermann and Marcelo Criscuolo and Carlos Eduardo Melo},

title = {High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow},

journal = {Journal of Biotechnology},

year = {2006},

volume = {124},

publisher = {Elsevier},

month = {jul},

url = {https://doi.org/10.1016%2Fj.jbiotec.2006.01.005},

number = {2},

pages = {469--472},

doi = {10.1016/j.jbiotec.2006.01.005}

}

MLA

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MLA Copy

Salamone, Daniel Felipe, et al. “High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow.” Journal of Biotechnology, vol. 124, no. 2, Jul. 2006, pp. 469-472. https://doi.org/10.1016%2Fj.jbiotec.2006.01.005.

Found error?

Publisher

Elsevier

Journal

Journal of Biotechnology

Quartile SCImago

Quartile WOS

Impact factor

4.1

ISSN

01681656 (Print)
18734863 (Electronic)