Journal of Biotechnology, volume 262, pages 84-88

The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose

Ortseifen Vera

Kalinowski Jörn ^{1, 2}

Pühler Alfred ³

Rückert Christian ¹

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Technology Platform Genomics, Center for Biotechnology, Bielefeld University, Sequenz 1, 33615 Bielefeld, Germany |

Microbial Genomics and Biotechnology, Center for Biotechnology, Bielefeld University, Universitätsstraße 27, 33615 Bielefeld, Germany. |

Senior Research Group Genome Research of Industrial Microorganisms, Center for Biotechnology, Bielefeld University, Universitätsstraße 27, 33615 Bielefeld, Germany |

Publication type: Journal Article

Publication date: 2017-11-01

Elsevier

Journal: Journal of Biotechnology

Quartile SCImago

Quartile WOS

Impact factor: 4.1

ISSN: 01681656, 18734863

DOI: 10.1016/j.jbiotec.2017.09.008

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General Medicine

Applied Microbiology and Biotechnology

Biotechnology

Bioengineering

Abstract

The secondary metabolite acarbose is used worldwide in the clinical treatment of diabetes mellitus type 2 patients. Acarbose is a - glucosidase inhibitor and supports patients to control their blood glucose as well as their serum insulin levels. The secondary metabolite is produced by strains of the class Actinobacteria, in particular from Actinoplanes sp. SE50/110, which is a progenitor of today`s production strains. Moreover, secondary metabolite clusters could also be identified in Streptomyces coelicoflavus ZG0656 as well as Streptomyces glaucescens GLA.O. In this study, the genome S. glaucescens GLA.O with focus on the acarbose biosynthesis cluster (gac-cluster) was analyzed. First, the tetracenomycin C and the 5`-hydroxy streptomycin gene clusters could be described completely. Then the gac gene region in S. glaucescens GLA.O is compared to the other known biosynthesis gene cluster. In comparison to Actinoplanes sp. SE50/110 the gac-cluster showed structural variances, like the missing homolog of the glycosyltransferase AcbD in the whole genome of S. glaucescens GLA.O. Due to the lack of the glycosyltransferase, it was of particular interest whether additional acarviose metabolites other than acarbose could be formed. For detection of acarviose metabolites biosynthesis the supernatant of S. glaucescens GLA.O grown in starch supplemented complex media was harvested at 72 and 96 hours. Although a homolog of the known glycosyltransferase is absent, the LC-MS-supported analysis revealed that a spectrum of acarviose metabolites was formed.

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Nature Chemical Biology	Nature Chemical Biology, 1, 10% Nature Chemical Biology 1 publication, 10%
Biochimie	Biochimie, 1, 10% Biochimie 1 publication, 10%
Marine Drugs	Marine Drugs, 1, 10% Marine Drugs 1 publication, 10%
Scientific Reports	Scientific Reports, 1, 10% Scientific Reports 1 publication, 10%
GeroScience	GeroScience, 1, 10% GeroScience 1 publication, 10%
Microorganisms	Microorganisms, 1, 10% Microorganisms 1 publication, 10%
Biotechnology Journal	Biotechnology Journal, 1, 10% Biotechnology Journal 1 publication, 10%
RSC Advances	RSC Advances, 1, 10% RSC Advances 1 publication, 10%
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Springer Nature	Springer Nature, 3, 30% Springer Nature 3 publications, 30%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 20% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 20%
Elsevier	Elsevier, 1, 10% Elsevier 1 publication, 10%
Wiley	Wiley, 1, 10% Wiley 1 publication, 10%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 10% Royal Society of Chemistry (RSC) 1 publication, 10%
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Ortseifen V. et al. The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose // Journal of Biotechnology. 2017. Vol. 262. pp. 84-88.

GOST all authors (up to 50) Copy

Ortseifen V., Kalinowski J., Pühler A., Rückert C. The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose // Journal of Biotechnology. 2017. Vol. 262. pp. 84-88.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1016/j.jbiotec.2017.09.008

UR - https://doi.org/10.1016%2Fj.jbiotec.2017.09.008

TI - The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose

T2 - Journal of Biotechnology

AU - Ortseifen, Vera

AU - Kalinowski, Jörn

AU - Pühler, Alfred

AU - Rückert, Christian

PY - 2017

DA - 2017/11/01 00:00:00

PB - Elsevier

SP - 84-88

VL - 262

SN - 0168-1656

SN - 1873-4863

ER -

BibTex

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BibTex Copy

@article{2017_Ortseifen,

author = {Vera Ortseifen and Jörn Kalinowski and Alfred Pühler and Christian Rückert},

title = {The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose},

journal = {Journal of Biotechnology},

year = {2017},

volume = {262},

publisher = {Elsevier},

month = {nov},

url = {https://doi.org/10.1016%2Fj.jbiotec.2017.09.008},

pages = {84--88},

doi = {10.1016/j.jbiotec.2017.09.008}

}

Found error?

Publisher

Elsevier

Journal

Journal of Biotechnology

Quartile SCImago

Quartile WOS

Impact factor

4.1

ISSN

01681656 (Print)
18734863 (Electronic)