Journal of Pharmaceutical and Biomedical Analysis, volume 40, issue 4, pages 1035-1039
APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness
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Department of Medicinal/Pharmaceutical Chemistry, University of Vienna, Pharmacy Center, Althanstrasse 14, A-1090 Vienna, Austria.
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Publication type: Journal Article
Publication date: 2006-03-01
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.4
ISSN: 07317085, 1873264X
Drug Discovery
Spectroscopy
Pharmaceutical Science
Clinical Biochemistry
Analytical Chemistry
Abstract
The aim of this work was the development of an easy manageable analytic system for describing tightness of cell layers in a molecular size dependent manner, which is more precise than currently used ones. Dextrans were labeled by reductive amination with fluorescent 1-aminopyrene-3,6,8-trisulfonate (APTS). This mixture, including internal standard diazepam, was used for transport studies, which were accomplished with an established transwell blood-brain barrier model culturing an immortalized porcine brain microvascular endothelial cell line (PBMEC/C1-2). Samples were analyzed by fluorescence measurements, capillary electrophoresis and RP-LC. Following this approach, a permeability pattern could be achieved including each single fraction from APTS, APTS-glucose to APTS-dextran consisting of 31 glucose units. Permeability coefficients were calculated and ranged from 16.38+/-3.79 microm/min for APTS to 6.07+/-1.23 microm/min for the APTS-dextran with 31 glucose units (diazepam: 67.97+/-7.32 microm/min). All in all, the developed APTS-dextran ladder is an useful tool to characterize cell layer tightness--especially to describe paracellular transport ways and leakiness status of the blood-brain barrier over time--applying a wide range from smaller to larger molecules at the same time in order to refine, e.g. TEER, sucrose or Evans blue measurements.
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Citations by publishers
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9 publications, 81.82%
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Springer Nature
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1 publication, 9.09%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
1 publication, 9.09%
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Neuhaus W. et al. APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness // Journal of Pharmaceutical and Biomedical Analysis. 2006. Vol. 40. No. 4. pp. 1035-1039.
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Neuhaus W., Trzeciak J., Lauer R., Lachmann B., Noe C. R. APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness // Journal of Pharmaceutical and Biomedical Analysis. 2006. Vol. 40. No. 4. pp. 1035-1039.
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TY - JOUR
DO - 10.1016/j.jpba.2005.08.023
UR - https://doi.org/10.1016%2Fj.jpba.2005.08.023
TI - APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness
T2 - Journal of Pharmaceutical and Biomedical Analysis
AU - Neuhaus, Winfried
AU - Trzeciak, Joanna
AU - Lauer, Regina
AU - Lachmann, Bodo
AU - Noe, Christian R
PY - 2006
DA - 2006/03/01 00:00:00
PB - Elsevier
SP - 1035-1039
IS - 4
VL - 40
SN - 0731-7085
SN - 1873-264X
ER -
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@article{2006_Neuhaus,
author = {Winfried Neuhaus and Joanna Trzeciak and Regina Lauer and Bodo Lachmann and Christian R Noe},
title = {APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness},
journal = {Journal of Pharmaceutical and Biomedical Analysis},
year = {2006},
volume = {40},
publisher = {Elsevier},
month = {mar},
url = {https://doi.org/10.1016%2Fj.jpba.2005.08.023},
number = {4},
pages = {1035--1039},
doi = {10.1016/j.jpba.2005.08.023}
}
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MLA
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Neuhaus, Winfried, et al. “APTS-labeled dextran ladder: A novel tool to characterize cell layer tightness.” Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 4, Mar. 2006, pp. 1035-1039. https://doi.org/10.1016%2Fj.jpba.2005.08.023.