Open Access
Molecular Cell, volume 56, issue 4, pages 531-540
Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome
Polikanov Yury S
1, 2
,
Osterman Ilya A
3
,
Szal Teresa
4
,
Kusochek Pavel
3
,
Bulkley David
5
,
Malanicheva Irina A
6
,
Efimenko Tatyana A
6
,
Konevega Andrey L.
7, 8
,
Shaw Karen J
9
,
Bogdanov Alexey A.
3
,
Rodnina Marina V.
10
,
Dontsova Olga A.
3
,
Steitz Thomas A.
2, 11
,
4
Center for Pharmaceutical Biotechnology, University of Illinois at Chicago, Chicago, IL 60607, USA
|
6
9
Hearts Consulting Group, San Diego, CA 92127, USA.
|
10
Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany
|
Publication type: Journal Article
Publication date: 2014-11-01
Journal:
Molecular Cell
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16
ISSN: 10972765, 10974164
PubMed ID:
25306919
Molecular Biology
Cell Biology
Abstract
We demonstrate that the antibiotic amicoumacin A (AMI) is a potent inhibitor of protein synthesis. Resistance mutations in helix 24 of the 16S rRNA mapped the AMI binding site to the small ribosomal subunit. The crystal structure of bacterial ribosome in complex with AMI solved at 2.4 Å resolution revealed that the antibiotic makes contacts with universally conserved nucleotides of 16S rRNA in the E site and the mRNA backbone. Simultaneous interactions of AMI with 16S rRNA and mRNA and the in vivo experimental evidence suggest that it may inhibit the progression of the ribosome along mRNA. Consistent with this proposal, binding of AMI interferes with translocation in vitro. The inhibitory action of AMI can be partly compensated by mutations in the translation elongation factor G.
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1 publication, 1.43%
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2
4
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8
10
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16
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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GOST
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Polikanov Y. S. et al. Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome // Molecular Cell. 2014. Vol. 56. No. 4. pp. 531-540.
GOST all authors (up to 50)
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Polikanov Y. S., Osterman I. A., Szal T., Tashlitsky V. N., Serebryakova M. V., Kusochek P., Bulkley D., Malanicheva I. A., Efimenko T. A., Ефременкова О. В., Konevega A. L., Shaw K. J., Bogdanov A. A., Rodnina M. V., Dontsova O. A., Mankin A. S., Steitz T. A., Sergiev P. V. Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome // Molecular Cell. 2014. Vol. 56. No. 4. pp. 531-540.
Cite this
RIS
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TY - JOUR
DO - 10.1016/j.molcel.2014.09.020
UR - https://doi.org/10.1016%2Fj.molcel.2014.09.020
TI - Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome
T2 - Molecular Cell
AU - Polikanov, Yury S
AU - Szal, Teresa
AU - Tashlitsky, Vadim N.
AU - Kusochek, Pavel
AU - Malanicheva, Irina A
AU - Efimenko, Tatyana A
AU - Ефременкова, О. В.
AU - Konevega, Andrey L.
AU - Shaw, Karen J
AU - Dontsova, Olga A.
AU - Osterman, Ilya A
AU - Serebryakova, M. V.
AU - Bulkley, David
AU - Bogdanov, Alexey A.
AU - Rodnina, Marina V.
AU - Mankin, Alexander S.
AU - Steitz, Thomas A.
AU - Sergiev, Petr V.
PY - 2014
DA - 2014/11/01 00:00:00
PB - Elsevier
SP - 531-540
IS - 4
VL - 56
PMID - 25306919
SN - 1097-2765
SN - 1097-4164
ER -
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@article{2014_Polikanov,
author = {Yury S Polikanov and Teresa Szal and Vadim N. Tashlitsky and Pavel Kusochek and Irina A Malanicheva and Tatyana A Efimenko and О. В. Ефременкова and Andrey L. Konevega and Karen J Shaw and Olga A. Dontsova and Ilya A Osterman and M. V. Serebryakova and David Bulkley and Alexey A. Bogdanov and Marina V. Rodnina and Alexander S. Mankin and Thomas A. Steitz and Petr V. Sergiev},
title = {Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome},
journal = {Molecular Cell},
year = {2014},
volume = {56},
publisher = {Elsevier},
month = {nov},
url = {https://doi.org/10.1016%2Fj.molcel.2014.09.020},
number = {4},
pages = {531--540},
doi = {10.1016/j.molcel.2014.09.020}
}
Cite this
MLA
Copy
Polikanov, Yury S., et al. “Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome.” Molecular Cell, vol. 56, no. 4, Nov. 2014, pp. 531-540. https://doi.org/10.1016%2Fj.molcel.2014.09.020.