Mutation Research - Genetic Toxicology and Environmental Mutagenesis, volume 858-860, pages 503222

N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.

Publication typeJournal Article
Publication date2020-10-01
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor1.9
ISSN13835718, 18793592
Genetics
Health, Toxicology and Mutagenesis
Abstract
Methoxychlor (MXC), an organo-chlorine insecticide, is a reproductive toxicant in females, causing apoptosis-mediated follicular atresia. To elucidate the potentials of Methoxychlor as a geno-toxicant, granulosa cells of healthy antral follicles, exposed to MXC and antioxidant, N-acetyl-l-cysteine, were studied by the terminal deoxynucleotidyltransferase-dUTP nick end-labelling and single-cell gel electrophoresis (comet) assays. MXC caused DNA fragmentation, as revealed by the increased incidence of dark brown condensed TUNEL positive cells in contrast with lightly brown TUNEL negative cells with maximum TUNEL positive cells were observed in 100 μg/mL MXC treated groups. Quantitatively, maximum geno-toxicity was exhibited at highest MXC treatment with percent tail DNA as 17.87 ± 0.85, 41.16 ± 3.94, and 47.73 ± 3.71 in comparison with control (0.65 ± 0.03, 2.91 ± 0.27, and 7.16 ± 1.39) after 24, 48 and 72 h exposure duration, respectively. MXC treated groups exhibited Type 1-Type 3 comets as compared to Type 0 comets in control groups. Supplementation of NAC led to significant (p < 0.05) decline in geno-toxicity in MXC treated groups with maximum amelioration observed at 5 and 10 mM. Consequently, increased DNA damage attributed to the granulosa cells apoptosis in response to Methoxychlor exposure was significantly combated by NAC supplementation, preventing the geno-toxicity induced cyto-toxicity in GCs.

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Bhardwaj J. K., Saraf P. N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles. // Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2020. Vol. 858-860. p. 503222.
GOST all authors (up to 50) Copy
Bhardwaj J. K., Saraf P. N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles. // Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2020. Vol. 858-860. p. 503222.
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RIS Copy
TY - JOUR
DO - 10.1016/j.mrgentox.2020.503222
UR - https://doi.org/10.1016%2Fj.mrgentox.2020.503222
TI - N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.
T2 - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
AU - Bhardwaj, J. K.
AU - Saraf, Priyanka
PY - 2020
DA - 2020/10/01 00:00:00
PB - Elsevier
SP - 503222
VL - 858-860
SN - 1383-5718
SN - 1879-3592
ER -
BibTex
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BibTex Copy
@article{2020_Bhardwaj
author = {J. K. Bhardwaj and Priyanka Saraf},
title = {N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.},
journal = {Mutation Research - Genetic Toxicology and Environmental Mutagenesis},
year = {2020},
volume = {858-860},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016%2Fj.mrgentox.2020.503222},
pages = {503222},
doi = {10.1016/j.mrgentox.2020.503222}
}
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