Mutation Research - Genetic Toxicology and Environmental Mutagenesis, volume 858-860, pages 503222
N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.
Publication type: Journal Article
Publication date: 2020-10-01
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor: 1.9
ISSN: 13835718, 18793592
Genetics
Health, Toxicology and Mutagenesis
Abstract
Methoxychlor (MXC), an organo-chlorine insecticide, is a reproductive toxicant in females, causing apoptosis-mediated follicular atresia. To elucidate the potentials of Methoxychlor as a geno-toxicant, granulosa cells of healthy antral follicles, exposed to MXC and antioxidant, N-acetyl-l-cysteine, were studied by the terminal deoxynucleotidyltransferase-dUTP nick end-labelling and single-cell gel electrophoresis (comet) assays. MXC caused DNA fragmentation, as revealed by the increased incidence of dark brown condensed TUNEL positive cells in contrast with lightly brown TUNEL negative cells with maximum TUNEL positive cells were observed in 100 μg/mL MXC treated groups. Quantitatively, maximum geno-toxicity was exhibited at highest MXC treatment with percent tail DNA as 17.87 ± 0.85, 41.16 ± 3.94, and 47.73 ± 3.71 in comparison with control (0.65 ± 0.03, 2.91 ± 0.27, and 7.16 ± 1.39) after 24, 48 and 72 h exposure duration, respectively. MXC treated groups exhibited Type 1-Type 3 comets as compared to Type 0 comets in control groups. Supplementation of NAC led to significant (p < 0.05) decline in geno-toxicity in MXC treated groups with maximum amelioration observed at 5 and 10 mM. Consequently, increased DNA damage attributed to the granulosa cells apoptosis in response to Methoxychlor exposure was significantly combated by NAC supplementation, preventing the geno-toxicity induced cyto-toxicity in GCs.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Bhardwaj J. K., Saraf P. N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles. // Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2020. Vol. 858-860. p. 503222.
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Bhardwaj J. K., Saraf P. N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles. // Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2020. Vol. 858-860. p. 503222.
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TY - JOUR
DO - 10.1016/j.mrgentox.2020.503222
UR - https://doi.org/10.1016%2Fj.mrgentox.2020.503222
TI - N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.
T2 - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
AU - Bhardwaj, J. K.
AU - Saraf, Priyanka
PY - 2020
DA - 2020/10/01 00:00:00
PB - Elsevier
SP - 503222
VL - 858-860
SN - 1383-5718
SN - 1879-3592
ER -
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@article{2020_Bhardwaj
author = {J. K. Bhardwaj and Priyanka Saraf},
title = {N-acetyl-l-cysteine mediated regulation of DNA fragmentation, an apoptotic event, against methoxychlor toxicity in the granulosa cells of ovarian antral follicles.},
journal = {Mutation Research - Genetic Toxicology and Environmental Mutagenesis},
year = {2020},
volume = {858-860},
publisher = {Elsevier},
month = {oct},
url = {https://doi.org/10.1016%2Fj.mrgentox.2020.503222},
pages = {503222},
doi = {10.1016/j.mrgentox.2020.503222}
}