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Saudi Journal of Biological Sciences

Aqueous extract of tobacco induces mitochondrial potential dependent cell death and epithelial-mesenchymal transition in gingival epithelial cells

Publication typeJournal Article
Publication date2021-05-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor4.4
ISSN1319562X
General Agricultural and Biological Sciences
Abstract
Smokeless tobacco habits are detrimental to oral health. A correlation between tobacco use and local epithelial tissue damage exists. Yet, the underlying cellular mechanism is not precisely characterized. This study assessed the dose-dependent action of Smokeless tobacco extract on gingival epithelial cells. Gingival tissue was taken from 5 healthy donors. Gingival epithelial cells were isolated by an enzymatic method and cultured up to passage 2. The cultured cells were treated with smokeless tobacco extract at 10%, 25%, 50%, and 75% volume concentration. After 48 h of incubation, MTT assay, Annexin V/PI assay, and DiIC1(5) assay were used to evaluate viability, apoptosis, and mitochondrial potential of the cells. RT-qPCR was used to determine the expression of BAX, BCL2, ECAD, NCAD, and TWIST. The Smokeless tobacco extract reduced cell viability by disrupting the mitochondrial potential and inducing apoptosis. Further, the Smokeless tobacco extract induced a dose-dependent epithelial-mesenchymal-transition in gingival epithelial cells. Apoptotic cellular death caused by tobacco extract on the gingival epithelial system was dependant on the mitochondrial potential of the cell. The results demonstrate that smokeless tobacco causes detrimental metabolic alterations of the periodontium. Featured application This study elucidates the mechanism by which Smokeless tobacco products cause cellular damage to the gingival epithelium. The use of Smokeless tobacco products can lead to major cellular and surface changes in the gingiva and its appearance. The consequences of these changes are not limited to oral cancer but also increases a person’s risk for dental and periodontal disease.

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GOST Copy
Patil S., Baeshen H. Aqueous extract of tobacco induces mitochondrial potential dependent cell death and epithelial-mesenchymal transition in gingival epithelial cells // Saudi Journal of Biological Sciences. 2021.
GOST all authors (up to 50) Copy
Patil S., Baeshen H. Aqueous extract of tobacco induces mitochondrial potential dependent cell death and epithelial-mesenchymal transition in gingival epithelial cells // Saudi Journal of Biological Sciences. 2021.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1016/j.sjbs.2021.04.068
UR - https://doi.org/10.1016%2Fj.sjbs.2021.04.068
TI - Aqueous extract of tobacco induces mitochondrial potential dependent cell death and epithelial-mesenchymal transition in gingival epithelial cells
T2 - Saudi Journal of Biological Sciences
AU - Patil, Shankargouda
AU - Baeshen, H.A.
PY - 2021
DA - 2021/05/01 00:00:00
PB - King Saud University
PMID - 34354447
SN - 1319-562X
ER -
BibTex
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BibTex Copy
@article{2021_Patil
author = {Shankargouda Patil and H.A. Baeshen},
title = {Aqueous extract of tobacco induces mitochondrial potential dependent cell death and epithelial-mesenchymal transition in gingival epithelial cells},
journal = {Saudi Journal of Biological Sciences},
year = {2021},
publisher = {King Saud University},
month = {may},
url = {https://doi.org/10.1016%2Fj.sjbs.2021.04.068},
doi = {10.1016/j.sjbs.2021.04.068}
}
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