Methods, volume 203, pages 431-446

CRISPR-Cas systems for diagnosing infectious diseases

Kostyusheva Anastasiya ¹

Brezgin Sergey ^{1, 2}

Babin Yuriy ¹

Vasilyeva I N ¹

Glebe Dieter ³

Kostyushev Dmitry ^{1, 4}

Chulanov Vladimir ^{1, 5}

Hide authors affiliations Show authors affiliations: 5 affiliations

National Medical Research Center of Tuberculosis and Infectious Diseases, Ministry of Health, Moscow, Russia |

Institute of Immunology, Moscow, Russia |

Institute of Medical Virology, University of Giessen, Giessen, Germany |

⁴

Sirius University of Science and Technology, Sochi, Russia |

⁵

Sechenov University, Moscow, Russia |

Publication type: Journal Article

Publication date: 2022-07-01

Elsevier

Journal: Methods

Quartile SCImago

Quartile WOS

Impact factor: 4.8

ISSN: 10462023, 10959130

DOI: 10.1016/j.ymeth.2021.04.007

Copy DOI

PubMed ID: 33839288

General Biochemistry, Genetics and Molecular Biology

Molecular Biology

Abstract

Infectious diseases are a global health problem affecting billions of people. Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing disease spread. Currently available diagnostics are very specific and sensitive but time-consuming and require expensive laboratory settings and well-trained personnel; thus, they are not available in resource-limited areas, for the purposes of large-scale screenings and in case of outbreaks and epidemics. Developing new, rapid, and affordable point-of-care diagnostic assays is urgently needed. This review focuses on CRISPR-based technologies and their perspectives to become platforms for point-of-care nucleic acid detection methods and as deployable diagnostic platforms that could help to identify and curb outbreaks and emerging epidemics. We describe the mechanisms and function of different classes and types of CRISPR-Cas systems, including pros and cons for developing molecular diagnostic tests and applications of each type to detect a wide range of infectious agents. Many Cas proteins (Cas3, Cas9, Cas12, Cas13, Cas14 etc.) have been leveraged to create highly accurate and sensitive diagnostic tools combined with technologies of signal amplification and fluorescent, potentiometric, colorimetric, lateral flow assay detection and other. In particular, the most advanced platforms -- SHERLOCK/v2, DETECTR, CARMEN or CRISPR-Chip -- enable detection of attomolar amounts of pathogenic nucleic acids with specificity comparable to that of PCR but with minimal technical settings. Further developing CRISPR-based diagnostic tools promises to dramatically transform molecular diagnostics, making them easily affordable and accessible virtually anywhere in the world. The burden of socially significant diseases, frequent outbreaks, recent epidemics (MERS, SARS and the ongoing COVID-19) and outbreaks of zoonotic viruses (African Swine Fever Virus etc.) urgently need the developing and distribution of express-diagnostic tools. Recently devised CRISPR-technologies represent the unprecedented opportunity to reshape epidemiological surveillance and molecular diagnostics.

By date By citations

Citations by journals

	1 2 3 4
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 4, 8% International Journal of Molecular Sciences 4 publications, 8%
Biosensors	Biosensors, 4, 8% Biosensors 4 publications, 8%
Analytical Chemistry	Analytical Chemistry, 3, 6% Analytical Chemistry 3 publications, 6%
Frontiers in Microbiology	Frontiers in Microbiology, 2, 4% Frontiers in Microbiology 2 publications, 4%
Microbiology spectrum	Microbiology spectrum, 2, 4% Microbiology spectrum 2 publications, 4%
Diagnostics	Diagnostics, 2, 4% Diagnostics 2 publications, 4%
Frontiers in Cellular and Infection Microbiology	Frontiers in Cellular and Infection Microbiology, 2, 4% Frontiers in Cellular and Infection Microbiology 2 publications, 4%
Biosensors and Bioelectronics	Biosensors and Bioelectronics, 2, 4% Biosensors and Bioelectronics 2 publications, 4%
The Analyst	The Analyst, 2, 4% The Analyst 2 publications, 4%
Talanta	Talanta, 2, 4% Talanta 2 publications, 4%
Bioengineering	Bioengineering, 1, 2% Bioengineering 1 publication, 2%
Genes	Genes, 1, 2% Genes 1 publication, 2%
Pathogens	Pathogens, 1, 2% Pathogens 1 publication, 2%
Talanta Open	Talanta Open, 1, 2% Talanta Open 1 publication, 2%
Agronomy	Agronomy, 1, 2% Agronomy 1 publication, 2%
Biomicrofluidics	Biomicrofluidics, 1, 2% Biomicrofluidics 1 publication, 2%
Molecular Ecology Resources	Molecular Ecology Resources, 1, 2% Molecular Ecology Resources 1 publication, 2%
EPMA Journal	EPMA Journal, 1, 2% EPMA Journal 1 publication, 2%
Applied Science and Convergence Technology	Applied Science and Convergence Technology, 1, 2% Applied Science and Convergence Technology 1 publication, 2%
Molecular Medicine Reports	Molecular Medicine Reports, 1, 2% Molecular Medicine Reports 1 publication, 2%
Sustainable Food Technology	Sustainable Food Technology, 1, 2% Sustainable Food Technology 1 publication, 2%
Sensors and Actuators, B: Chemical	Sensors and Actuators, B: Chemical, 1, 2% Sensors and Actuators, B: Chemical 1 publication, 2%
ACS Omega	ACS Omega, 1, 2% ACS Omega 1 publication, 2%
Infection	Infection, 1, 2% Infection 1 publication, 2%
Analytical Methods	Analytical Methods, 1, 2% Analytical Methods 1 publication, 2%
PLoS ONE	PLoS ONE, 1, 2% PLoS ONE 1 publication, 2%
Clinical Chemistry and Laboratory Medicine	Clinical Chemistry and Laboratory Medicine, 1, 2% Clinical Chemistry and Laboratory Medicine 1 publication, 2%
Journal of Medical Virology	Journal of Medical Virology, 1, 2% Journal of Medical Virology 1 publication, 2%
AIDS Research and Human Retroviruses	AIDS Research and Human Retroviruses, 1, 2% AIDS Research and Human Retroviruses 1 publication, 2%
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Citations by publishers

	2 4 6 8 10 12 14
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 14, 28% Multidisciplinary Digital Publishing Institute (MDPI) 14 publications, 28%
Elsevier	Elsevier, 9, 18% Elsevier 9 publications, 18%
Frontiers Media S.A.	Frontiers Media S.A., 4, 8% Frontiers Media S.A. 4 publications, 8%
American Chemical Society (ACS)	American Chemical Society (ACS), 4, 8% American Chemical Society (ACS) 4 publications, 8%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 4, 8% Royal Society of Chemistry (RSC) 4 publications, 8%
American Society for Microbiology	American Society for Microbiology, 2, 4% American Society for Microbiology 2 publications, 4%
Wiley	Wiley, 2, 4% Wiley 2 publications, 4%
Springer Nature	Springer Nature, 2, 4% Springer Nature 2 publications, 4%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 1, 2% American Institute of Physics (AIP) 1 publication, 2%
The Korean Vacuum Society	The Korean Vacuum Society, 1, 2% The Korean Vacuum Society 1 publication, 2%
Spandidos Publications	Spandidos Publications, 1, 2% Spandidos Publications 1 publication, 2%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 1, 2% Public Library of Science (PLoS) 1 publication, 2%
Walter de Gruyter	Walter de Gruyter, 1, 2% Walter de Gruyter 1 publication, 2%
Mary Ann Liebert	Mary Ann Liebert, 1, 2% Mary Ann Liebert 1 publication, 2%
Pleiades Publishing	Pleiades Publishing, 1, 2% Pleiades Publishing 1 publication, 2%
	2 4 6 8 10 12 14

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Kostyusheva A. et al. CRISPR-Cas systems for diagnosing infectious diseases // Methods. 2022. Vol. 203. pp. 431-446.

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Kostyusheva A., Brezgin S., Babin Y., Vasilyeva I. N., Glebe D., Kostyushev D., Chulanov V. CRISPR-Cas systems for diagnosing infectious diseases // Methods. 2022. Vol. 203. pp. 431-446.

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RIS Copy

TY - JOUR

DO - 10.1016/j.ymeth.2021.04.007

UR - https://doi.org/10.1016%2Fj.ymeth.2021.04.007

TI - CRISPR-Cas systems for diagnosing infectious diseases

T2 - Methods

AU - Kostyusheva, Anastasiya

AU - Brezgin, Sergey

AU - Babin, Yuriy

AU - Vasilyeva, I N

AU - Glebe, Dieter

AU - Kostyushev, Dmitry

AU - Chulanov, Vladimir

PY - 2022

DA - 2022/07/01 00:00:00

PB - Elsevier

SP - 431-446

VL - 203

PMID - 33839288

SN - 1046-2023

SN - 1095-9130

ER -

BibTex

Cite this

BibTex Copy

@article{2022_Kostyusheva,

author = {Anastasiya Kostyusheva and Sergey Brezgin and Yuriy Babin and I N Vasilyeva and Dieter Glebe and Dmitry Kostyushev and Vladimir Chulanov},

title = {CRISPR-Cas systems for diagnosing infectious diseases},

journal = {Methods},

year = {2022},

volume = {203},

publisher = {Elsevier},

month = {jul},

url = {https://doi.org/10.1016%2Fj.ymeth.2021.04.007},

pages = {431--446},

doi = {10.1016/j.ymeth.2021.04.007}

}

Found error?

Publisher

Elsevier

Journal

Methods

Quartile SCImago

Quartile WOS

Impact factor

4.8

ISSN

10462023 (Print)
10959130 (Electronic)

Labs

Laboratory of Genetic Technologies

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