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ACS Nano, volume 4, issue 10, pages 5897-5904

Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes

Wu Chung-Shieh 1, 2
Oo Maung Htun 1
Fan Xudong 1
1
 
Biomedical Engineering Department, University of Michigan, 1101 Beal Avenue, Ann Arbor, Michigan 48109, United States
2
 
Department of Biological Engineering, Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, United States
Publication typeJournal Article
Publication date2010-10-06
Journal: ACS Nano
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor17.1
ISSN19360851, 1936086X
General Physics and Astronomy
General Materials Science
General Engineering
Abstract
We developed highly sensitive and specific nanosensors based on quantum dots (QDs) and DNAzyme for multiplexed detection of heavy metal ions in liquid. The QDs were coated with a thin silica layer for increased stability and higher quantum yield while maintaining a relatively small size for highly efficient energy transfer. The QD-DNAzyme nanosensors were constructed by conjugating quencher-labeled DNAzymes onto the surface of carboxyl-silanized QDs. In the presence of metal ions, the emission is restored due to the cleavage of DNAzymes. The detection could be completed within 25 min with a single laser excitation source. The detection limit of 0.2 and 0.5 nM was experimentally achieved for Pb(2+) and Cu(2+), respectively, which is a 50- and 70-fold improvement over the recent results obtained with dye molecules. Multiplexed detection was also demonstrated using two different colors of QDs, showing negligible cross-talk between the Pb(2+) detection and Cu(2+) detection.

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Wu C. et al. Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes // ACS Nano. 2010. Vol. 4. No. 10. pp. 5897-5904.
GOST all authors (up to 50) Copy
Wu C., Oo M. H., Fan X. Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes // ACS Nano. 2010. Vol. 4. No. 10. pp. 5897-5904.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1021/nn1021988
UR - https://doi.org/10.1021%2Fnn1021988
TI - Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes
T2 - ACS Nano
AU - Wu, Chung-Shieh
AU - Oo, Maung Htun
AU - Fan, Xudong
PY - 2010
DA - 2010/10/06 00:00:00
PB - American Chemical Society (ACS)
SP - 5897-5904
IS - 10
VL - 4
SN - 1936-0851
SN - 1936-086X
ER -
BibTex |
Cite this
BibTex Copy
@article{2010_Wu
author = {Chung-Shieh Wu and Maung Htun Oo and Xudong Fan},
title = {Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes},
journal = {ACS Nano},
year = {2010},
volume = {4},
publisher = {American Chemical Society (ACS)},
month = {oct},
url = {https://doi.org/10.1021%2Fnn1021988},
number = {10},
pages = {5897--5904},
doi = {10.1021/nn1021988}
}
MLA
Cite this
MLA Copy
Wu, Chung-Shieh, et al. “Highly Sensitive Multiplexed Heavy Metal Detection Using Quantum-Dot-Labeled DNAzymes.” ACS Nano, vol. 4, no. 10, Oct. 2010, pp. 5897-5904. https://doi.org/10.1021%2Fnn1021988.
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