Nature Methods, volume 5, issue 4, pages 311-313
In vivo immunogold labeling confirms large-scale chromatin folding motifs
Kireev Igor
1, 2
,
Lakonishok Margot
1, 3
,
Liu Wenqiu
4
,
Joshi Vishwas N.
4
,
Powell Rick
4
,
Belmont Andrew S.
1
1
Department of Cell and Developmental Biology, University of Illinois, Urbana-Champaign, Urbana, USA
|
3
Present address: Department of Molecular and Cell Biology, Northwestern Medical School, 303 E. Chicago Ave., Ward 11-080, Chicago, Illinois 60611, USA.,
|
4
Nanoprobes, Inc., Yaphank, USA
|
Publication type: Journal Article
Publication date: 2008-03-16
Journal:
Nature Methods
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 48
ISSN: 15487091, 15487105
Biochemistry
Molecular Biology
Cell Biology
Biotechnology
Abstract
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP–proliferating cell nuclear antigen (PCNA).
Citations by journals
1
2
3
|
|
Current Opinion in Cell Biology
|
Current Opinion in Cell Biology
3 publications, 8.82%
|
Cell and Tissue Biology
|
Cell and Tissue Biology
2 publications, 5.88%
|
Journal of Structural Biology
|
Journal of Structural Biology
2 publications, 5.88%
|
Methods in Molecular Biology
|
Methods in Molecular Biology
2 publications, 5.88%
|
Bulletin of Experimental Biology and Medicine
|
Bulletin of Experimental Biology and Medicine
1 publication, 2.94%
|
Journal of Cell Biology
|
Journal of Cell Biology
1 publication, 2.94%
|
BioNanoScience
|
BioNanoScience
1 publication, 2.94%
|
Current Biology
|
Current Biology
1 publication, 2.94%
|
Frontiers in Cell and Developmental Biology
|
Frontiers in Cell and Developmental Biology
1 publication, 2.94%
|
Frontiers in Immunology
|
Frontiers in Immunology
1 publication, 2.94%
|
BMC Cell Biology
|
BMC Cell Biology
1 publication, 2.94%
|
Chromosoma
|
Chromosoma
1 publication, 2.94%
|
Journal of Biological Chemistry
|
Journal of Biological Chemistry
1 publication, 2.94%
|
Structure
|
Structure
1 publication, 2.94%
|
Experimental Cell Research
|
Experimental Cell Research
1 publication, 2.94%
|
Trends in Biochemical Sciences
|
Trends in Biochemical Sciences
1 publication, 2.94%
|
Micron
|
Micron
1 publication, 2.94%
|
BioEssays
|
BioEssays
1 publication, 2.94%
|
Wiley Interdisciplinary Reviews: Systems Biology and Medicine
|
Wiley Interdisciplinary Reviews: Systems Biology and Medicine
1 publication, 2.94%
|
FEBS Journal
|
FEBS Journal
1 publication, 2.94%
|
EMBO Journal
|
EMBO Journal
1 publication, 2.94%
|
Brain Pathology
|
Brain Pathology
1 publication, 2.94%
|
Nucleus
|
Nucleus
1 publication, 2.94%
|
International Review of Cell and Molecular Biology
|
International Review of Cell and Molecular Biology
1 publication, 2.94%
|
Nucleic Acids Research
|
Nucleic Acids Research
1 publication, 2.94%
|
Cold Spring Harbor perspectives in biology
|
Cold Spring Harbor perspectives in biology
1 publication, 2.94%
|
1
2
3
|
Citations by publishers
2
4
6
8
10
12
|
|
Elsevier
|
Elsevier
11 publications, 32.35%
|
Springer Nature
|
Springer Nature
6 publications, 17.65%
|
Wiley
|
Wiley
4 publications, 11.76%
|
Pleiades Publishing
|
Pleiades Publishing
2 publications, 5.88%
|
Frontiers Media S.A.
|
Frontiers Media S.A.
2 publications, 5.88%
|
Rockefeller University Press
|
Rockefeller University Press
1 publication, 2.94%
|
American Society for Biochemistry and Molecular Biology
|
American Society for Biochemistry and Molecular Biology
1 publication, 2.94%
|
EMBO press
|
EMBO press
1 publication, 2.94%
|
Taylor & Francis
|
Taylor & Francis
1 publication, 2.94%
|
Oxford University Press
|
Oxford University Press
1 publication, 2.94%
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
1 publication, 2.94%
|
2
4
6
8
10
12
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021,2022],"ids":[0,0,0,0,0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[5,2,3,6,4,3,3,3,2,1,0,0,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["14.71","5.88","8.82","17.65","11.76","8.82","8.82","8.82","5.88","2.94",0,0,"2.94","2.94"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Current Opinion in Cell Biology","Cell and Tissue Biology","Journal of Structural Biology","Methods in Molecular Biology","Bulletin of Experimental Biology and Medicine","Journal of Cell Biology","BioNanoScience","Current Biology","Frontiers in Cell and Developmental Biology","Frontiers in Immunology","BMC Cell Biology","Chromosoma","Journal of Biological Chemistry","Structure","Experimental Cell Research","Trends in Biochemical Sciences","Micron","BioEssays","Wiley Interdisciplinary Reviews: Systems Biology and Medicine","FEBS Journal","EMBO Journal","Brain Pathology","Nucleus","International Review of Cell and Molecular Biology","Nucleic Acids Research","Cold Spring Harbor perspectives in biology"],"ids":[7206,16847,15912,3516,7563,867,9784,16010,12128,1699,9570,10452,19451,5828,525,4668,913,11017,10622,9481,16016,23785,5407,11492,23904,21200],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp"],"datasets":[{"label":"","data":[3,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[8.82,5.88,5.88,5.88,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Springer Nature","Wiley","Pleiades Publishing","Frontiers Media S.A.","Rockefeller University Press","American Society for Biochemistry and Molecular Biology","EMBO press","Taylor & Francis","Oxford University Press","Cold Spring Harbor Laboratory"],"ids":[17,8,11,101,208,22,2090,9415,18,19,6909],"codes":[0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/lEA1FyUnFAZR1DchuczZi5bjAixG3TNeWVz6oF4R_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/1UiI5HycPsvulDeXOySNp0OuaMMmIKfPnsdKPyH0_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp"],"datasets":[{"label":"","data":[11,6,4,2,2,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[32.35,17.65,11.76,5.88,5.88,2.94,2.94,2.94,2.94,2.94,2.94],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kireev I. et al. In vivo immunogold labeling confirms large-scale chromatin folding motifs // Nature Methods. 2008. Vol. 5. No. 4. pp. 311-313.
GOST all authors (up to 50)
Copy
Kireev I., Lakonishok M., Liu W., Joshi V. N., Powell R., Belmont A. S. In vivo immunogold labeling confirms large-scale chromatin folding motifs // Nature Methods. 2008. Vol. 5. No. 4. pp. 311-313.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1038/NMETH.1196
UR - https://doi.org/10.1038%2FNMETH.1196
TI - In vivo immunogold labeling confirms large-scale chromatin folding motifs
T2 - Nature Methods
AU - Lakonishok, Margot
AU - Liu, Wenqiu
AU - Joshi, Vishwas N.
AU - Kireev, Igor
AU - Belmont, Andrew S.
AU - Powell, Rick
PY - 2008
DA - 2008/03/16 00:00:00
PB - Springer Nature
SP - 311-313
IS - 4
VL - 5
SN - 1548-7091
SN - 1548-7105
ER -
Cite this
BibTex
Copy
@article{2008_Kireev
author = {Margot Lakonishok and Wenqiu Liu and Vishwas N. Joshi and Igor Kireev and Andrew S. Belmont and Rick Powell},
title = {In vivo immunogold labeling confirms large-scale chromatin folding motifs},
journal = {Nature Methods},
year = {2008},
volume = {5},
publisher = {Springer Nature},
month = {mar},
url = {https://doi.org/10.1038%2FNMETH.1196},
number = {4},
pages = {311--313},
doi = {10.1038/NMETH.1196}
}
Cite this
MLA
Copy
Kireev, Igor, et al. “In vivo immunogold labeling confirms large-scale chromatin folding motifs.” Nature Methods, vol. 5, no. 4, Mar. 2008, pp. 311-313. https://doi.org/10.1038%2FNMETH.1196.
Profiles