Nature Methods, volume 5, issue 4, pages 311-313

In vivo immunogold labeling confirms large-scale chromatin folding motifs

Kireev Igor 1, 2
Lakonishok Margot 1, 3
Liu Wenqiu 4
Joshi Vishwas N. 4
Powell Rick 4
Belmont Andrew S. 1
1
 
Department of Cell and Developmental Biology, University of Illinois, Urbana-Champaign, Urbana, USA
3
 
Present address: Department of Molecular and Cell Biology, Northwestern Medical School, 303 E. Chicago Ave., Ward 11-080, Chicago, Illinois 60611, USA.,
4
 
Nanoprobes, Inc., Yaphank, USA
Publication typeJournal Article
Publication date2008-03-16
Journal: Nature Methods
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor48
ISSN15487091, 15487105
Biochemistry
Molecular Biology
Cell Biology
Biotechnology
Abstract
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP–proliferating cell nuclear antigen (PCNA).

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GOST |
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GOST Copy
Kireev I. et al. In vivo immunogold labeling confirms large-scale chromatin folding motifs // Nature Methods. 2008. Vol. 5. No. 4. pp. 311-313.
GOST all authors (up to 50) Copy
Kireev I., Lakonishok M., Liu W., Joshi V. N., Powell R., Belmont A. S. In vivo immunogold labeling confirms large-scale chromatin folding motifs // Nature Methods. 2008. Vol. 5. No. 4. pp. 311-313.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1038/NMETH.1196
UR - https://doi.org/10.1038%2FNMETH.1196
TI - In vivo immunogold labeling confirms large-scale chromatin folding motifs
T2 - Nature Methods
AU - Lakonishok, Margot
AU - Liu, Wenqiu
AU - Joshi, Vishwas N.
AU - Kireev, Igor
AU - Belmont, Andrew S.
AU - Powell, Rick
PY - 2008
DA - 2008/03/16 00:00:00
PB - Springer Nature
SP - 311-313
IS - 4
VL - 5
SN - 1548-7091
SN - 1548-7105
ER -
BibTex |
Cite this
BibTex Copy
@article{2008_Kireev
author = {Margot Lakonishok and Wenqiu Liu and Vishwas N. Joshi and Igor Kireev and Andrew S. Belmont and Rick Powell},
title = {In vivo immunogold labeling confirms large-scale chromatin folding motifs},
journal = {Nature Methods},
year = {2008},
volume = {5},
publisher = {Springer Nature},
month = {mar},
url = {https://doi.org/10.1038%2FNMETH.1196},
number = {4},
pages = {311--313},
doi = {10.1038/NMETH.1196}
}
MLA
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MLA Copy
Kireev, Igor, et al. “In vivo immunogold labeling confirms large-scale chromatin folding motifs.” Nature Methods, vol. 5, no. 4, Mar. 2008, pp. 311-313. https://doi.org/10.1038%2FNMETH.1196.
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