Journal of Antibiotics, volume 71, issue 2, pages 333-338
Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster
Reynolds Kirk A.
1, 2
,
Luhavaya Hanna
1
,
Li Jie
1
,
Dahesh Samira
3
,
Nizet Victor
3, 4
,
Yamanaka Kazuya
1, 5
,
Moore Bradley Matthys
1, 4
1
Scripps Institution of Oceanography, University of California at San Diego, La Jolla, USA
|
2
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, USA
|
3
Department of Pediatrics, University of California at San Diego, La Jolla, USA
|
4
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, USA
|
5
6Current address: Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, Japan.,
|
Publication type: Journal Article
Publication date: 2017-11-29
Journal:
Journal of Antibiotics
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.3
ISSN: 00218820, 18811469
PubMed ID:
29184121
Drug Discovery
Pharmacology
Abstract
In the ongoing effort to unlock the chemical potential of marine bacteria, genetic engineering of biosynthetic gene clusters (BGCs) is increasingly used to awake or improve expression of biosynthetic genes that may lead to discovery of novel bioactive natural products. Previously, we reported the successful capture, engineering and heterologous expression of an orphan BGC from the marine actinomycete Saccharomonospora sp. CNQ-490, which resulted in the isolation of the novel lipopeptide antibiotic taromycin A. Herein we report the isolation and structure elucidation of taromycin B, the second most abundant product of the taromycin biosynthetic series, and show that taromycins A and B exhibit complex chromatographic properties indicative of interconverting conformations. Taromycins A and B display potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium clinical isolates, suggestive that the taromycin molecular scaffold is a promising starting point for further derivatization to produce compounds with promising antibiotic characteristics.
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1 publication, 1.72%
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1 publication, 1.72%
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mSystems
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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Microorganisms
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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Chemistry - A European Journal
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1 publication, 1.72%
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Chinese Journal of Chemistry
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1 publication, 1.72%
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1
2
3
4
5
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Citations by publishers
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2
4
6
8
10
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10 publications, 17.24%
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Wiley
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7 publications, 12.07%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
7 publications, 12.07%
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Royal Society of Chemistry (RSC)
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7 publications, 12.07%
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Springer Nature
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6 publications, 10.34%
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Frontiers Media S.A.
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3 publications, 5.17%
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Taylor & Francis
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2 publications, 3.45%
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1 publication, 1.72%
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IOP Publishing
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IOP Publishing
1 publication, 1.72%
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American Society for Pharmacology and Experimental Therapeutics
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American Society for Pharmacology and Experimental Therapeutics
1 publication, 1.72%
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SAGE
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SAGE
1 publication, 1.72%
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2
4
6
8
10
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Reynolds K. A. et al. Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster // Journal of Antibiotics. 2017. Vol. 71. No. 2. pp. 333-338.
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Reynolds K. A., Luhavaya H., Li J., Dahesh S., Nizet V., Yamanaka K., Moore B. M. Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster // Journal of Antibiotics. 2017. Vol. 71. No. 2. pp. 333-338.
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TY - JOUR
DO - 10.1038/ja.2017.146
UR - https://doi.org/10.1038%2Fja.2017.146
TI - Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster
T2 - Journal of Antibiotics
AU - Reynolds, Kirk A.
AU - Luhavaya, Hanna
AU - Li, Jie
AU - Dahesh, Samira
AU - Nizet, Victor
AU - Yamanaka, Kazuya
AU - Moore, Bradley Matthys
PY - 2017
DA - 2017/11/29 00:00:00
PB - Springer Nature
SP - 333-338
IS - 2
VL - 71
PMID - 29184121
SN - 0021-8820
SN - 1881-1469
ER -
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@article{2017_Reynolds,
author = {Kirk A. Reynolds and Hanna Luhavaya and Jie Li and Samira Dahesh and Victor Nizet and Kazuya Yamanaka and Bradley Matthys Moore},
title = {Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster},
journal = {Journal of Antibiotics},
year = {2017},
volume = {71},
publisher = {Springer Nature},
month = {nov},
url = {https://doi.org/10.1038%2Fja.2017.146},
number = {2},
pages = {333--338},
doi = {10.1038/ja.2017.146}
}
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MLA
Copy
Reynolds, Kirk A., et al. “Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster.” Journal of Antibiotics, vol. 71, no. 2, Nov. 2017, pp. 333-338. https://doi.org/10.1038%2Fja.2017.146.