Nature, volume 427, issue 6977, pages 862-865
Visualization of release factor 3 on the ribosome during termination of protein synthesis
2
Department of Structural Biology and Genomics, Institute of Genetics and Molecular and Cellular Biology, CNRS/INSERM/ULP, Illkirch, France
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Publication type: Journal Article
Publication date: 2004-02-25
Multidisciplinary
Abstract
Termination of protein synthesis by the ribosome requires two release factor (RF) classes. The class II RF3 is a GTPase that removes class I RFs (RF1 or RF2) from the ribosome after release of the nascent polypeptide1,2,3. RF3 in the GDP state binds to the ribosomal class I RF complex, followed by an exchange of GDP for GTP and release of the class I RF. As GTP hydrolysis triggers release of RF3 (ref. 4), we trapped RF3 on Escherichia coli ribosomes using a nonhydrolysable GTP analogue. Here we show by cryo-electron microscopy that the complex can adopt two different conformational states. In ‘state 1’, RF3 is pre-bound to the ribosome, whereas in ‘state 2’ RF3 contacts the ribosome GTPase centre. The transfer RNA molecule translocates from the peptidyl site in state 1 to the exit site in state 2. This translocation is associated with a large conformational rearrangement of the ribosome. Because state 1 seems able to accommodate simultaneously both RF3 and RF2, whose position is known from previous studies5,6, we can infer the release mechanism of class I RFs.
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Klaholz B. P. et al. Visualization of release factor 3 on the ribosome during termination of protein synthesis // Nature. 2004. Vol. 427. No. 6977. pp. 862-865.
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Klaholz B. P., Myasnikov A. G., van Heel M. Visualization of release factor 3 on the ribosome during termination of protein synthesis // Nature. 2004. Vol. 427. No. 6977. pp. 862-865.
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TY - JOUR
DO - 10.1038/nature02332
UR - https://doi.org/10.1038%2Fnature02332
TI - Visualization of release factor 3 on the ribosome during termination of protein synthesis
T2 - Nature
AU - Klaholz, Bruno P.
AU - Myasnikov, Alexander G.
AU - van Heel, Marin
PY - 2004
DA - 2004/02/25 00:00:00
PB - Springer Nature
SP - 862-865
IS - 6977
VL - 427
SN - 0028-0836
SN - 1476-4687
ER -
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@article{2004_Klaholz
author = {Bruno P. Klaholz and Alexander G. Myasnikov and Marin van Heel},
title = {Visualization of release factor 3 on the ribosome during termination of protein synthesis},
journal = {Nature},
year = {2004},
volume = {427},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1038%2Fnature02332},
number = {6977},
pages = {862--865},
doi = {10.1038/nature02332}
}
Cite this
MLA
Copy
Klaholz, Bruno P., et al. “Visualization of release factor 3 on the ribosome during termination of protein synthesis.” Nature, vol. 427, no. 6977, Feb. 2004, pp. 862-865. https://doi.org/10.1038%2Fnature02332.