Nature, volume 427, issue 6977, pages 862-865

Visualization of release factor 3 on the ribosome during termination of protein synthesis

Klaholz Bruno P. 1, 2
Myasnikov Alexander G. 3
van Heel Marin 1
Publication typeJournal Article
Publication date2004-02-25
Journal: Nature
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor64.8
ISSN00280836, 14764687
Multidisciplinary
Abstract
Termination of protein synthesis by the ribosome requires two release factor (RF) classes. The class II RF3 is a GTPase that removes class I RFs (RF1 or RF2) from the ribosome after release of the nascent polypeptide1,2,3. RF3 in the GDP state binds to the ribosomal class I RF complex, followed by an exchange of GDP for GTP and release of the class I RF. As GTP hydrolysis triggers release of RF3 (ref. 4), we trapped RF3 on Escherichia coli ribosomes using a nonhydrolysable GTP analogue. Here we show by cryo-electron microscopy that the complex can adopt two different conformational states. In ‘state 1’, RF3 is pre-bound to the ribosome, whereas in ‘state 2’ RF3 contacts the ribosome GTPase centre. The transfer RNA molecule translocates from the peptidyl site in state 1 to the exit site in state 2. This translocation is associated with a large conformational rearrangement of the ribosome. Because state 1 seems able to accommodate simultaneously both RF3 and RF2, whose position is known from previous studies5,6, we can infer the release mechanism of class I RFs.

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Klaholz B. P. et al. Visualization of release factor 3 on the ribosome during termination of protein synthesis // Nature. 2004. Vol. 427. No. 6977. pp. 862-865.
GOST all authors (up to 50) Copy
Klaholz B. P., Myasnikov A. G., van Heel M. Visualization of release factor 3 on the ribosome during termination of protein synthesis // Nature. 2004. Vol. 427. No. 6977. pp. 862-865.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1038/nature02332
UR - https://doi.org/10.1038%2Fnature02332
TI - Visualization of release factor 3 on the ribosome during termination of protein synthesis
T2 - Nature
AU - Klaholz, Bruno P.
AU - Myasnikov, Alexander G.
AU - van Heel, Marin
PY - 2004
DA - 2004/02/25 00:00:00
PB - Springer Nature
SP - 862-865
IS - 6977
VL - 427
SN - 0028-0836
SN - 1476-4687
ER -
BibTex |
Cite this
BibTex Copy
@article{2004_Klaholz
author = {Bruno P. Klaholz and Alexander G. Myasnikov and Marin van Heel},
title = {Visualization of release factor 3 on the ribosome during termination of protein synthesis},
journal = {Nature},
year = {2004},
volume = {427},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1038%2Fnature02332},
number = {6977},
pages = {862--865},
doi = {10.1038/nature02332}
}
MLA
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MLA Copy
Klaholz, Bruno P., et al. “Visualization of release factor 3 on the ribosome during termination of protein synthesis.” Nature, vol. 427, no. 6977, Feb. 2004, pp. 862-865. https://doi.org/10.1038%2Fnature02332.
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