Nature, volume 455, issue 7213, pages 633-637

Structure of the Tribolium castaneum telomerase catalytic subunit TERT

Gillis Andrew J 1
Schuller Anthony P 1
Skordalakes Emmanuel 1
1
 
Gene Expression and Regulation Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA ,
Publication typeJournal Article
Publication date2008-08-31
Journal: Nature
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor64.8
ISSN00280836, 14764687
Multidisciplinary
Abstract
A common hallmark of human cancers is the overexpression of telomerase, a ribonucleoprotein complex that is responsible for maintaining the length and integrity of chromosome ends. Telomere length deregulation and telomerase activation is an early, and perhaps necessary, step in cancer cell evolution. Here we present the high-resolution structure of the Tribolium castaneum catalytic subunit of telomerase, TERT. The protein consists of three highly conserved domains, organized into a ring-like structure that shares common features with retroviral reverse transcriptases, viral RNA polymerases and B-family DNA polymerases. Domain organization places motifs implicated in substrate binding and catalysis in the interior of the ring, which can accommodate seven to eight bases of double-stranded nucleic acid. Modelling of an RNA–DNA heteroduplex in the interior of this ring demonstrates a perfect fit between the protein and the nucleic acid substrate, and positions the 3′-end of the DNA primer at the active site of the enzyme, providing evidence for the formation of an active telomerase elongation complex.

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Gillis A. J., Schuller A. P., Skordalakes E. Structure of the Tribolium castaneum telomerase catalytic subunit TERT // Nature. 2008. Vol. 455. No. 7213. pp. 633-637.
GOST all authors (up to 50) Copy
Gillis A. J., Schuller A. P., Skordalakes E. Structure of the Tribolium castaneum telomerase catalytic subunit TERT // Nature. 2008. Vol. 455. No. 7213. pp. 633-637.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1038/nature07283
UR - https://doi.org/10.1038%2Fnature07283
TI - Structure of the Tribolium castaneum telomerase catalytic subunit TERT
T2 - Nature
AU - Gillis, Andrew J
AU - Schuller, Anthony P
AU - Skordalakes, Emmanuel
PY - 2008
DA - 2008/08/31 00:00:00
PB - Springer Nature
SP - 633-637
IS - 7213
VL - 455
SN - 0028-0836
SN - 1476-4687
ER -
BibTex |
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BibTex Copy
@article{2008_Gillis,
author = {Andrew J Gillis and Anthony P Schuller and Emmanuel Skordalakes},
title = {Structure of the Tribolium castaneum telomerase catalytic subunit TERT},
journal = {Nature},
year = {2008},
volume = {455},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1038%2Fnature07283},
number = {7213},
pages = {633--637},
doi = {10.1038/nature07283}
}
MLA
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MLA Copy
Gillis, Andrew J., et al. “Structure of the Tribolium castaneum telomerase catalytic subunit TERT.” Nature, vol. 455, no. 7213, Aug. 2008, pp. 633-637. https://doi.org/10.1038%2Fnature07283.
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