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Nature Communications, volume 5, issue 1, publication number 4263

DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes

Yamamoto Junichi ¹

Hagiwara Yuri ¹

Chiba Kunitoshi ¹

Isobe Tomoyasu ¹

Narita Takashi ²

Handa Hiroshi ³

Yamaguchi Yuki ^{1, 4}

Hide authors affiliations Show authors affiliations: 4 affiliations

Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan |

Graduate School of Frontier Biosciences, Osaka University, Suita, Japan |

Department of Nanoparticle Translational Research, Tokyo Medical University, Tokyo, Japan |

⁴

PRESTO, Japan Science and Technology Agency, Kawaguchi, Japan |

Publication type: Journal Article

Publication date: 2014-06-27

Springer Nature

Journal: Nature Communications

Quartile SCImago

Quartile WOS

Impact factor: 16.6

ISSN: 20411723

DOI: 10.1038/ncomms5263

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General Chemistry

General Biochemistry, Genetics and Molecular Biology

General Physics and Astronomy

Abstract

The elongation factors DSIF and NELF are responsible for promoter-proximal RNA polymerase II (Pol II) pausing. NELF is also involved in 3' processing of replication-dependent histone genes, which produce non-polyadenylated mRNAs. Here we show that DSIF and NELF contribute to the synthesis of small nuclear RNAs (snRNAs) through their association with Integrator, the large multisubunit complex responsible for 3' processing of pre-snRNAs. In HeLa cells, Pol II, Integrator, DSIF and NELF accumulate at the 3' end of the U1 snRNA gene. Knockdown of NELF results in misprocessing of U1, U2, U4 and U5 snRNAs, while DSIF is required for proper transcription of these genes. Knocking down NELF also disrupts transcription termination and induces the production of polyadenylated U1 transcripts caused by an enhanced recruitment of cleavage stimulation factor. Our results indicate that NELF plays a key role in determining the post-transcriptional fate of Pol II-transcribed genes.

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Citations by journals

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Nature Reviews Molecular Cell Biology	Nature Reviews Molecular Cell Biology, 3, 4.17% Nature Reviews Molecular Cell Biology 3 publications, 4.17%
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Molecular and Cellular Biology	Molecular and Cellular Biology, 2, 2.78% Molecular and Cellular Biology 2 publications, 2.78%
Science	Science, 2, 2.78% Science 2 publications, 2.78%
Proceedings of the National Academy of Sciences of the United States of America	Proceedings of the National Academy of Sciences of the United States of America, 2, 2.78% Proceedings of the National Academy of Sciences of the United States of America 2 publications, 2.78%
Cells	Cells, 1, 1.39% Cells 1 publication, 1.39%
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Biochemical Society Transactions	Biochemical Society Transactions, 1, 1.39% Biochemical Society Transactions 1 publication, 1.39%
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Biomolecules	Biomolecules, 1, 1.39% Biomolecules 1 publication, 1.39%
BMC Biology	BMC Biology, 1, 1.39% BMC Biology 1 publication, 1.39%
Cell Research	Cell Research, 1, 1.39% Cell Research 1 publication, 1.39%
Nature	Nature, 1, 1.39% Nature 1 publication, 1.39%
BioScience Trends	BioScience Trends, 1, 1.39% BioScience Trends 1 publication, 1.39%
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms	Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1, 1.39% Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 1 publication, 1.39%
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BioEssays	BioEssays, 1, 1.39% BioEssays 1 publication, 1.39%
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Citations by publishers

	5 10 15 20
Elsevier	Elsevier, 20, 27.78% Elsevier 20 publications, 27.78%
Springer Nature	Springer Nature, 14, 19.44% Springer Nature 14 publications, 19.44%
Taylor & Francis	Taylor & Francis, 4, 5.56% Taylor & Francis 4 publications, 5.56%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 4.17% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 4.17%
Wiley	Wiley, 3, 4.17% Wiley 3 publications, 4.17%
Oxford University Press	Oxford University Press, 3, 4.17% Oxford University Press 3 publications, 4.17%
Cold Spring Harbor Laboratory	Cold Spring Harbor Laboratory, 3, 4.17% Cold Spring Harbor Laboratory 3 publications, 4.17%
American Society for Microbiology	American Society for Microbiology, 2, 2.78% American Society for Microbiology 2 publications, 2.78%
American Association for the Advancement of Science (AAAS)	American Association for the Advancement of Science (AAAS), 2, 2.78% American Association for the Advancement of Science (AAAS) 2 publications, 2.78%
Proceedings of the National Academy of Sciences (PNAS)	Proceedings of the National Academy of Sciences (PNAS), 2, 2.78% Proceedings of the National Academy of Sciences (PNAS) 2 publications, 2.78%
Canadian Science Publishing	Canadian Science Publishing, 1, 1.39% Canadian Science Publishing 1 publication, 1.39%
Portland Press	Portland Press, 1, 1.39% Portland Press 1 publication, 1.39%
The Royal Society	The Royal Society, 1, 1.39% The Royal Society 1 publication, 1.39%
International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA)	International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA), 1, 1.39% International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA) 1 publication, 1.39%
Pleiades Publishing	Pleiades Publishing, 1, 1.39% Pleiades Publishing 1 publication, 1.39%
eLife Sciences Publications	eLife Sciences Publications, 1, 1.39% eLife Sciences Publications 1 publication, 1.39%
American Society of Hematology	American Society of Hematology, 1, 1.39% American Society of Hematology 1 publication, 1.39%
	5 10 15 20

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Yamamoto J. et al. DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes // Nature Communications. 2014. Vol. 5. No. 1. 4263

GOST all authors (up to 50) Copy

Yamamoto J., Hagiwara Y., Chiba K., Isobe T., Narita T., Handa H., Yamaguchi Y. DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes // Nature Communications. 2014. Vol. 5. No. 1. 4263

RIS |

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RIS Copy

TY - JOUR

DO - 10.1038/ncomms5263

UR - https://doi.org/10.1038%2Fncomms5263

TI - DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes

T2 - Nature Communications

AU - Yamamoto, Junichi

AU - Hagiwara, Yuri

AU - Chiba, Kunitoshi

AU - Isobe, Tomoyasu

AU - Narita, Takashi

AU - Handa, Hiroshi

AU - Yamaguchi, Yuki

PY - 2014

DA - 2014/06/27 00:00:00

PB - Springer Nature

IS - 1

VL - 5

SN - 2041-1723

ER -

BibTex

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BibTex Copy

@article{2014_Yamamoto,

author = {Junichi Yamamoto and Yuri Hagiwara and Kunitoshi Chiba and Tomoyasu Isobe and Takashi Narita and Hiroshi Handa and Yuki Yamaguchi},

title = {DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes},

journal = {Nature Communications},

year = {2014},

volume = {5},

publisher = {Springer Nature},

month = {jun},

url = {https://doi.org/10.1038%2Fncomms5263},

number = {1},

doi = {10.1038/ncomms5263}

}

Found error?

Publisher

Springer Nature

Journal

Nature Communications

Quartile SCImago

Quartile WOS

Impact factor

16.6

ISSN

20411723 (Print)