Open Access
Nature Structural and Molecular Biology, volume 21, issue 6, pages 507-512
Structural basis for protein-RNA recognition in telomerase
Huang Jing
1, 2, 3
,
Brown Andrew F.
4
,
Wu Jian
1
,
Jing Xue
1
,
Bley Christopher J.
4
,
Rand Dustin P
4
,
Wu Lijie
1
,
Zhang Rongguang
1
,
Chen Julian J L
4
,
Lei Ming
1, 2, 3
1
National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
|
2
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, USA
|
3
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, USA
|
4
Department of Chemistry and Biochemistry, Arizona State University, Tempe, USA
|
Publication type: Journal Article
Publication date: 2014-05-04
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.8
ISSN: 15459993, 15459985
PubMed ID:
24793650
Molecular Biology
Structural Biology
Abstract
Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal structure of the conserved regions 4 and 5 (CR4/5) of TR in complex with the TR-binding domain (TRBD) of TERT from the teleost fish Oryzias latipes. The structure shows that CR4/5 adopts an L-shaped three-way-junction conformation with its two arms clamping onto TRBD. Both the sequence and conformation of CR4/5 are required for the interaction. Our structural and mutational analyses suggest that the observed CR4/5-TRBD recognition is common to most eukaryotes, and CR4/5 in vertebrate TR might have a similar role in telomerase regulation as that of stem-loop IV in Tetrahymena TR.
Citations by journals
|
1
2
3
4
5
|
|
|
Nucleic Acids Research
|
Nucleic Acids Research
5 publications, 7.35%
|
|
International Journal of Molecular Sciences
|
International Journal of Molecular Sciences
3 publications, 4.41%
|
|
Nature Structural and Molecular Biology
|
Nature Structural and Molecular Biology
3 publications, 4.41%
|
|
Wiley interdisciplinary reviews. RNA
|
Wiley interdisciplinary reviews. RNA
3 publications, 4.41%
|
|
Biomolecules
|
Biomolecules
2 publications, 2.94%
|
|
Molecules
|
Molecules
2 publications, 2.94%
|
|
Nature
|
Nature
2 publications, 2.94%
|
|
Journal of Biological Chemistry
|
Journal of Biological Chemistry
2 publications, 2.94%
|
|
Cell
|
Cell
2 publications, 2.94%
|
|
RNA Biology
|
RNA Biology
2 publications, 2.94%
|
|
Proceedings of the National Academy of Sciences of the United States of America
|
Proceedings of the National Academy of Sciences of the United States of America
2 publications, 2.94%
|
|
Blood
|
Blood
2 publications, 2.94%
|
|
RNA
|
RNA
2 publications, 2.94%
|
|
Cold Spring Harbor perspectives in biology
|
Cold Spring Harbor perspectives in biology
2 publications, 2.94%
|
|
Biomedicines
|
Biomedicines
1 publication, 1.47%
|
|
Biochemical Journal
|
Biochemical Journal
1 publication, 1.47%
|
|
Biochemical Society Transactions
|
Biochemical Society Transactions
1 publication, 1.47%
|
|
Cells
|
Cells
1 publication, 1.47%
|
|
Cell Research
|
Cell Research
1 publication, 1.47%
|
|
Mobile DNA
|
Mobile DNA
1 publication, 1.47%
|
|
Cellular and Molecular Life Sciences
|
Cellular and Molecular Life Sciences
1 publication, 1.47%
|
|
Nature Communications
|
Nature Communications
1 publication, 1.47%
|
|
PLoS Genetics
|
PLoS Genetics
1 publication, 1.47%
|
|
Developmental Cell
|
Developmental Cell
1 publication, 1.47%
|
|
Structure
|
Structure
1 publication, 1.47%
|
|
Pharmacology and Therapeutics
|
Pharmacology and Therapeutics
1 publication, 1.47%
|
|
Cell Reports
|
Cell Reports
1 publication, 1.47%
|
|
Current Opinion in Structural Biology
|
Current Opinion in Structural Biology
1 publication, 1.47%
|
|
Materials Today: Proceedings
|
Materials Today: Proceedings
1 publication, 1.47%
|
|
1
2
3
4
5
|
Citations by publishers
|
2
4
6
8
10
12
|
|
|
Springer Nature
|
Springer Nature
11 publications, 16.18%
|
|
Elsevier
|
Elsevier
10 publications, 14.71%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
9 publications, 13.24%
|
|
Oxford University Press
|
Oxford University Press
6 publications, 8.82%
|
|
Wiley
|
Wiley
5 publications, 7.35%
|
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
5 publications, 7.35%
|
|
Portland Press
|
Portland Press
2 publications, 2.94%
|
|
American Society for Biochemistry and Molecular Biology
|
American Society for Biochemistry and Molecular Biology
2 publications, 2.94%
|
|
Taylor & Francis
|
Taylor & Francis
2 publications, 2.94%
|
|
Proceedings of the National Academy of Sciences (PNAS)
|
Proceedings of the National Academy of Sciences (PNAS)
2 publications, 2.94%
|
|
American Society of Hematology
|
American Society of Hematology
2 publications, 2.94%
|
|
Annual Reviews
|
Annual Reviews
2 publications, 2.94%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
1 publication, 1.47%
|
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
1 publication, 1.47%
|
|
American Society for Microbiology
|
American Society for Microbiology
1 publication, 1.47%
|
|
American Association for the Advancement of Science (AAAS)
|
American Association for the Advancement of Science (AAAS)
1 publication, 1.47%
|
|
2
4
6
8
10
12
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2014,2015,2016,2017,2018,2019,2020,2021,2022,2023,2024],"ids":[0,0,0,0,0,0,0,0,0,0,0],"codes":[0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","","","","","","","","","",""],"datasets":[{"label":"Citations number","data":[1,10,11,9,5,6,9,8,4,4,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["1.47","14.71","16.18","13.24","7.35","8.82","13.24","11.76","5.88","5.88","1.47"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Nucleic Acids Research","International Journal of Molecular Sciences","Nature Structural and Molecular Biology","Wiley interdisciplinary reviews. RNA","Biomolecules","Molecules","Nature","Journal of Biological Chemistry","Cell","RNA Biology","Proceedings of the National Academy of Sciences of the United States of America","Blood","RNA","Cold Spring Harbor perspectives in biology","Biomedicines","Biochemical Journal","Biochemical Society Transactions","Cells","Cell Research","Mobile DNA","Cellular and Molecular Life Sciences","Nature Communications","PLoS Genetics","Developmental Cell","Structure","Pharmacology and Therapeutics","Cell Reports","Current Opinion in Structural Biology","Materials Today: Proceedings"],"ids":[23904,14627,13408,8123,13780,1770,14611,19451,16552,7613,306,6594,12892,21200,24421,15459,10198,25293,10892,2865,5151,3231,785,17069,5828,22929,9130,13702,24147],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/QMhhcrIUiVtFnND3WWZ7hIbu6GYeoRoA2zoVsMEX_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[5,3,3,3,2,2,2,2,2,2,2,2,2,2,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[7.35,4.41,4.41,4.41,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,2.94,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47,1.47],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Springer Nature","Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Oxford University Press","Wiley","Cold Spring Harbor Laboratory","Portland Press","American Society for Biochemistry and Molecular Biology","Taylor & Francis","Proceedings of the National Academy of Sciences (PNAS)","American Society of Hematology","Annual Reviews","Public Library of Science (PLoS)","American Chemical Society (ACS)","American Society for Microbiology","American Association for the Advancement of Science (AAAS)"],"ids":[8,17,202,19,11,6909,801,2090,18,162,2252,6941,344,40,142,189],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/yNSijlgQghQF53VZuyFLA30CKDe4j3HK74Vtpnxa_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/nUqNrP9GnM1t0BrAIXp1LBodjU8aIJ2Fk4pLfGhZ_medium.webp","\/storage\/images\/resized\/OuA6WPZekwEiUM99mP2pSqj4yHPxHezM6xgLhAjO_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/mxFdPe9qujsfvfYfcN0QOclAiYORFb0xrRlwV8gs_medium.webp","\/storage\/images\/resized\/QMhhcrIUiVtFnND3WWZ7hIbu6GYeoRoA2zoVsMEX_medium.webp","\/storage\/images\/resized\/A7oGMwx1m3fFJlKJvQUVra8h0itmEa7JZW0q3Q0b_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp"],"datasets":[{"label":"","data":[11,10,9,6,5,5,2,2,2,2,2,2,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.18,14.71,13.24,8.82,7.35,7.35,2.94,2.94,2.94,2.94,2.94,2.94,1.47,1.47,1.47,1.47],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Huang J. et al. Structural basis for protein-RNA recognition in telomerase // Nature Structural and Molecular Biology. 2014. Vol. 21. No. 6. pp. 507-512.
GOST all authors (up to 50)
Copy
Huang J., Brown A. F., Wu J., Jing X., Bley C. J., Rand D. P., Wu L., Zhang R., Chen J. J. L., Lei M. Structural basis for protein-RNA recognition in telomerase // Nature Structural and Molecular Biology. 2014. Vol. 21. No. 6. pp. 507-512.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1038/nsmb.2819
UR - https://doi.org/10.1038%2Fnsmb.2819
TI - Structural basis for protein-RNA recognition in telomerase
T2 - Nature Structural and Molecular Biology
AU - Huang, Jing
AU - Brown, Andrew F.
AU - Wu, Jian
AU - Jing, Xue
AU - Bley, Christopher J.
AU - Rand, Dustin P
AU - Wu, Lijie
AU - Zhang, Rongguang
AU - Chen, Julian J L
AU - Lei, Ming
PY - 2014
DA - 2014/05/04 00:00:00
PB - Springer Nature
SP - 507-512
IS - 6
VL - 21
PMID - 24793650
SN - 1545-9993
SN - 1545-9985
ER -
Cite this
BibTex
Copy
@article{2014_Huang,
author = {Jing Huang and Andrew F. Brown and Jian Wu and Xue Jing and Christopher J. Bley and Dustin P Rand and Lijie Wu and Rongguang Zhang and Julian J L Chen and Ming Lei},
title = {Structural basis for protein-RNA recognition in telomerase},
journal = {Nature Structural and Molecular Biology},
year = {2014},
volume = {21},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1038%2Fnsmb.2819},
number = {6},
pages = {507--512},
doi = {10.1038/nsmb.2819}
}
Cite this
MLA
Copy
Huang, Jing, et al. “Structural basis for protein-RNA recognition in telomerase.” Nature Structural and Molecular Biology, vol. 21, no. 6, May. 2014, pp. 507-512. https://doi.org/10.1038%2Fnsmb.2819.