Nature Structural and Molecular Biology, volume 13, issue 3, pages 218-225
Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase
1
Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, USA
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Publication type: Journal Article
Publication date: 2006-02-05
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.8
ISSN: 15459993, 15459985
DOI:
10.1038/nsmb1054
Molecular Biology
Structural Biology
Abstract
Telomerase, a ribonucleoprotein enzyme, adds telomeric DNA repeats to the ends of linear chromosomes. Here we report the first high-resolution structure of any portion of the telomerase reverse transcriptase, the telomerase essential N-terminal (TEN) domain from Tetrahymena thermophila. The structure, which seems to represent a novel protein fold, shows phylogenetically conserved amino acid residues in a groove on its surface. These residues are crucial for telomerase catalytic activity, and several of them are required for sequence-specific binding of a single-stranded telomeric DNA primer. The positively charged C terminus, which becomes ordered upon interaction with other macromolecules, is involved in binding RNA in a non–sequence-specific manner. The TEN domain's ability to bind both RNA and telomeric DNA, coupled with the notably strong effects on activity upon mutagenesis of single surface residues, suggest how this domain contributes to telomerase catalysis.
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Jacobs S. A., Podell E. R., Cech T. R. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase // Nature Structural and Molecular Biology. 2006. Vol. 13. No. 3. pp. 218-225.
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Jacobs S. A., Podell E. R., Cech T. R. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase // Nature Structural and Molecular Biology. 2006. Vol. 13. No. 3. pp. 218-225.
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TY - JOUR
DO - 10.1038/nsmb1054
UR - https://doi.org/10.1038%2Fnsmb1054
TI - Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase
T2 - Nature Structural and Molecular Biology
AU - Jacobs, Steven A.
AU - Podell, Elaine R.
AU - Cech, Thomas R
PY - 2006
DA - 2006/02/05 00:00:00
PB - Springer Nature
SP - 218-225
IS - 3
VL - 13
SN - 1545-9993
SN - 1545-9985
ER -
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@article{2006_Jacobs,
author = {Steven A. Jacobs and Elaine R. Podell and Thomas R Cech},
title = {Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase},
journal = {Nature Structural and Molecular Biology},
year = {2006},
volume = {13},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1038%2Fnsmb1054},
number = {3},
pages = {218--225},
doi = {10.1038/nsmb1054}
}
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MLA
Copy
Jacobs, Steven A., et al. “Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase.” Nature Structural and Molecular Biology, vol. 13, no. 3, Feb. 2006, pp. 218-225. https://doi.org/10.1038%2Fnsmb1054.