Open Access
Nature Communications, volume 11, issue 1, publication number 156
Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
Nieto Blanca
1, 2
,
Gaspar Sonia G
1, 2
,
Moriggi Giulia
1, 2
,
Pestov Dimitri G.
3
,
Bustelo Xosé R.
1, 2, 4
,
Dosil Mercedes
1, 2, 4, 5
1
Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, CSIC-University of Salamanca, Salamanca, Spain
|
2
Instituto de Biología Molecular y Celular del Cáncer, CSIC-University of Salamanca, Salamanca, Spain
|
3
Department of Cell Biology and Neuroscience, Rowan University School of Osteopathic Medicine, Stratford, USA
|
4
Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), CSIC-University of Salamanca, Salamanca, Spain
|
5
Departamento de Bioquímica y Biología Molecular, University of Salamanca, Salamanca, Spain
|
Publication type: Journal Article
Publication date: 2020-01-09
Journal:
Nature Communications
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16.6
ISSN: 20411723
PubMed ID:
31919354
General Chemistry
General Biochemistry, Genetics and Molecular Biology
Multidisciplinary
General Physics and Astronomy
Abstract
Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit. Ribosome synthesis is a complex multi-step process. Here the authors present a method that allows the efficient isolation and characterization of the preribosomal complexes formed along the entire ribosome synthesis pathway in human cells.
Citations by journals
|
1
|
|
|
Acta Naturae
|
Acta Naturae, 1, 5.56%
Acta Naturae
1 publication, 5.56%
|
|
Life
|
Life
1 publication, 5.56%
|
|
Biomedicines
|
Biomedicines
1 publication, 5.56%
|
|
Frontiers in Microbiology
|
Frontiers in Microbiology
1 publication, 5.56%
|
|
Frontiers in Oncology
|
Frontiers in Oncology
1 publication, 5.56%
|
|
Current Opinion in Structural Biology
|
Current Opinion in Structural Biology
1 publication, 5.56%
|
|
PLoS Genetics
|
PLoS Genetics
1 publication, 5.56%
|
|
Wiley interdisciplinary reviews. RNA
|
Wiley interdisciplinary reviews. RNA
1 publication, 5.56%
|
|
Biology Bulletin Reviews
|
Biology Bulletin Reviews
1 publication, 5.56%
|
|
RNA Biology
|
RNA Biology
1 publication, 5.56%
|
|
RNA
|
RNA
1 publication, 5.56%
|
|
Cell Reports
|
Cell Reports
1 publication, 5.56%
|
|
Cells
|
Cells
1 publication, 5.56%
|
|
Scientific Reports
|
Scientific Reports
1 publication, 5.56%
|
|
1
|
Citations by publishers
|
1
2
3
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 16.67%
|
|
Frontiers Media S.A.
|
Frontiers Media S.A.
2 publications, 11.11%
|
|
Elsevier
|
Elsevier
2 publications, 11.11%
|
|
Acta Naturae Ltd
|
Acta Naturae Ltd, 1, 5.56%
Acta Naturae Ltd
1 publication, 5.56%
|
|
Public Library of Science (PLoS)
|
Public Library of Science (PLoS)
1 publication, 5.56%
|
|
Wiley
|
Wiley
1 publication, 5.56%
|
|
Pleiades Publishing
|
Pleiades Publishing
1 publication, 5.56%
|
|
Taylor & Francis
|
Taylor & Francis
1 publication, 5.56%
|
|
Cold Spring Harbor Laboratory
|
Cold Spring Harbor Laboratory
1 publication, 5.56%
|
|
Springer Nature
|
Springer Nature
1 publication, 5.56%
|
|
1
2
3
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[2,6,5,3,2],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["11.11","33.33","27.78","16.67","11.11"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Acta Naturae","Life","Biomedicines","Frontiers in Microbiology","Frontiers in Oncology","Current Opinion in Structural Biology","PLoS Genetics","Wiley interdisciplinary reviews. RNA","Biology Bulletin Reviews","RNA Biology","RNA","Cell Reports","Cells","Scientific Reports"],"ids":[7401,23944,24421,24403,10990,13702,785,8123,25559,7613,12892,9130,25293,13767],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56,5.56],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Frontiers Media S.A.","Elsevier","Acta Naturae Ltd","Public Library of Science (PLoS)","Wiley","Pleiades Publishing","Taylor & Francis","Cold Spring Harbor Laboratory","Springer Nature"],"ids":[202,208,17,7905,344,11,101,18,6909,8],"codes":[0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/bRyGpdm98BkAUYiK1YFNpl5Z7hPu6Gd87gbIeuG3_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/5YZtvLvkPZuc2JHOaZsjCvGSHFCuC3drUwN3YAc5_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[3,2,2,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.67,11.11,11.11,5.56,5.56,5.56,5.56,5.56,5.56,5.56],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Nieto B. et al. Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis // Nature Communications. 2020. Vol. 11. No. 1. 156
GOST all authors (up to 50)
Copy
Nieto B., Gaspar S. G., Moriggi G., Pestov D. G., Bustelo X. R., Dosil M. Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis // Nature Communications. 2020. Vol. 11. No. 1. 156
Cite this
RIS
Copy
TY - JOUR
DO - 10.1038/s41467-019-13990-w
UR - https://doi.org/10.1038%2Fs41467-019-13990-w
TI - Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
T2 - Nature Communications
AU - Nieto, Blanca
AU - Gaspar, Sonia G
AU - Moriggi, Giulia
AU - Pestov, Dimitri G.
AU - Bustelo, Xosé R.
AU - Dosil, Mercedes
PY - 2020
DA - 2020/01/09 00:00:00
PB - Springer Nature
IS - 1
VL - 11
PMID - 31919354
SN - 2041-1723
ER -
Cite this
BibTex
Copy
@article{2020_Nieto,
author = {Blanca Nieto and Sonia G Gaspar and Giulia Moriggi and Dimitri G. Pestov and Xosé R. Bustelo and Mercedes Dosil},
title = {Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis},
journal = {Nature Communications},
year = {2020},
volume = {11},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1038%2Fs41467-019-13990-w},
number = {1},
doi = {10.1038/s41467-019-13990-w}
}