Open Access
Scientific Reports, volume 9, issue 1, publication number 3722
PARP1 is a versatile factor in the regulation of mRNA stability and decay
1
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, USA
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Publication type: Journal Article
Publication date: 2019-03-06
PubMed ID:
30842529
Multidisciplinary
Abstract
PARP1 is an abundant nuclear protein with many pleiotropic functions involved in epigenetic and transcriptional controls. Abundance of mRNA depends on the balance between synthesis and decay of a particular transcript. PARP1 binds RNA and its depletion results in increased expression of genes involved in nonsense-mediated decay, suggesting that PARP1 might be involved in mRNA stability. This is of interest considering RNA binding proteins play key roles in post-transcriptional processes in all eukaryotes. We tested the direct impact of PARP1 and PARylation on mRNA stability and decay. By measuring the half-lives of two PARP1-mRNA targets we found that the half-lives were significantly decreased in PARP1-depleted cells. PARP1 depletion impacted both the synthesis of nascent mRNA and the stability of mature mRNAs. PARylation impacted the production of nascent mRNA and the stability of mature mRNA, albeit to a lesser extent than PARP1 KD. PARylation enhanced the impact of PARP1 depletion. These studies provide the first direct comparative role of PARP1 and PARylation in RNA stability and decay, adding a new dimension as to how PARP1 regulates gene expression. These studies present a platform to begin to tease out the influence of PARP1 at each step of RNA biogenesis and decay to fine-tune gene expression.
Citations by journals
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3
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Cells
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3 publications, 14.29%
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Wiley interdisciplinary reviews. RNA
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International Journal of Molecular Sciences
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Biology
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3 Biotech
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3 Biotech
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Cellular and Molecular Life Sciences
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Journal of Hematology and Oncology
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Molecular Cell
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Molecular Cell
1 publication, 4.76%
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DNA Repair
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DNA Repair
1 publication, 4.76%
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FEBS Letters
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FEBS Letters
1 publication, 4.76%
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Blood advances
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1 publication, 4.76%
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NAR Cancer
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NAR Cancer
1 publication, 4.76%
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Cell Reports
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Cell Reports
1 publication, 4.76%
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Nucleic Acids Research
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Nucleic Acids Research
1 publication, 4.76%
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Journal of Alzheimer's Disease
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Journal of Alzheimer's Disease
1 publication, 4.76%
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3
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Citations by publishers
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5
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
5 publications, 23.81%
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Springer Nature
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Springer Nature
3 publications, 14.29%
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Elsevier
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Elsevier
3 publications, 14.29%
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Wiley
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Wiley
3 publications, 14.29%
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Oxford University Press
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Oxford University Press
2 publications, 9.52%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 4.76%
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Wolters Kluwer Health
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Wolters Kluwer Health
1 publication, 4.76%
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American Society of Hematology
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American Society of Hematology
1 publication, 4.76%
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IOS Press
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IOS Press
1 publication, 4.76%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Matveeva E. A. et al. PARP1 is a versatile factor in the regulation of mRNA stability and decay // Scientific Reports. 2019. Vol. 9. No. 1. 3722
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Matveeva E. A., Mathbout L. F., Fondufe Mittendorf Y. N. PARP1 is a versatile factor in the regulation of mRNA stability and decay // Scientific Reports. 2019. Vol. 9. No. 1. 3722
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TY - JOUR
DO - 10.1038/s41598-019-39969-7
UR - https://doi.org/10.1038%2Fs41598-019-39969-7
TI - PARP1 is a versatile factor in the regulation of mRNA stability and decay
T2 - Scientific Reports
AU - Matveeva, Elena A.
AU - Mathbout, Lein F
AU - Fondufe Mittendorf, Yvonne N
PY - 2019
DA - 2019/03/06 00:00:00
PB - Springer Nature
IS - 1
VL - 9
PMID - 30842529
SN - 2045-2322
ER -
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@article{2019_Matveeva,
author = {Elena A. Matveeva and Lein F Mathbout and Yvonne N Fondufe Mittendorf},
title = {PARP1 is a versatile factor in the regulation of mRNA stability and decay},
journal = {Scientific Reports},
year = {2019},
volume = {9},
publisher = {Springer Nature},
month = {mar},
url = {https://doi.org/10.1038%2Fs41598-019-39969-7},
number = {1},
doi = {10.1038/s41598-019-39969-7}
}