Open Access
Open access
Biochemical Journal, volume 418, issue 3, pages 567-574

Far-red fluorescent tags for protein imaging in living tissues

Shcherbo Dmitry 1
Murphy Christopher S. 2
Ermakova Galina V. 1
Solovieva Elena A. 1
Chepurnykh Tatiana V. 3
Shcheglov Aleksandr S. 1
Verkhusha Vladislav V. 4
Pletnev Vladimir Z. 1
Hazelwood Kristin L. 2
Roche Patrick M. 2
Lukyanov Sergey 1
Zaraisky Andrey G. 1
Davidson Michael W. 2
Chudakov Dmitriy M. 1
Publication typeJournal Article
Publication date2009-01-15
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor4.1
ISSN02646021, 14708728, 03063283, 00062936
PubMed ID:  19143658
Biochemistry
Molecular Biology
Cell Biology
Abstract
A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper, we report mKate2, a monomeric far-red fluorescent protein that is almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. The high-brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues. We also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum. Together, monomeric mKate2 and pseudo-monomeric tdKatushka2 represent the next generation of extra-bright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals.

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Shcherbo D. et al. Far-red fluorescent tags for protein imaging in living tissues // Biochemical Journal. 2009. Vol. 418. No. 3. pp. 567-574.
GOST all authors (up to 50) Copy
Shcherbo D., Murphy C., Ermakova G., Solovieva E., Chepurnykh T., Shcheglov A., Verkhusha V. V., Pletnev V., Hazelwood K., Roche P., Lukyanov S., Zaraisky A., Davidson M., Chudakov D. M. Far-red fluorescent tags for protein imaging in living tissues // Biochemical Journal. 2009. Vol. 418. No. 3. pp. 567-574.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1042/BJ20081949
UR - https://doi.org/10.1042%2FBJ20081949
TI - Far-red fluorescent tags for protein imaging in living tissues
T2 - Biochemical Journal
AU - Shcherbo, Dmitry
AU - Murphy, Christopher S.
AU - Ermakova, Galina V.
AU - Solovieva, Elena A.
AU - Chepurnykh, Tatiana V.
AU - Shcheglov, Aleksandr S.
AU - Verkhusha, Vladislav V.
AU - Pletnev, Vladimir Z.
AU - Hazelwood, Kristin L.
AU - Roche, Patrick M.
AU - Lukyanov, Sergey
AU - Zaraisky, Andrey G.
AU - Davidson, Michael W.
AU - Chudakov, Dmitriy M.
PY - 2009
DA - 2009/01/15 00:00:00
PB - Portland Press
SP - 567-574
IS - 3
VL - 418
PMID - 19143658
SN - 0264-6021
SN - 1470-8728
SN - 0306-3283
SN - 0006-2936
ER -
BibTex |
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BibTex Copy
@article{2009_Shcherbo
author = {Dmitry Shcherbo and Christopher S. Murphy and Galina V. Ermakova and Elena A. Solovieva and Tatiana V. Chepurnykh and Aleksandr S. Shcheglov and Vladislav V. Verkhusha and Vladimir Z. Pletnev and Kristin L. Hazelwood and Patrick M. Roche and Sergey Lukyanov and Andrey G. Zaraisky and Michael W. Davidson and Dmitriy M. Chudakov},
title = {Far-red fluorescent tags for protein imaging in living tissues},
journal = {Biochemical Journal},
year = {2009},
volume = {418},
publisher = {Portland Press},
month = {jan},
url = {https://doi.org/10.1042%2FBJ20081949},
number = {3},
pages = {567--574},
doi = {10.1042/BJ20081949}
}
MLA
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MLA Copy
Shcherbo, Dmitry, et al. “Far-red fluorescent tags for protein imaging in living tissues.” Biochemical Journal, vol. 418, no. 3, Jan. 2009, pp. 567-574. https://doi.org/10.1042%2FBJ20081949.
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