Assay and Drug Development Technologies, volume 6, issue 5, pages 637-657

A Robotic Platform for Quantitative High-Throughput Screening

Michael Sam 1
Auld Douglas 1
Klumpp Carleen 1
Jadhav Ajit 1
Zheng Wei 1
Zheng Wei 1
Thorne Natasha 1
Austin Chris 1
Inglese J. 1
Simeonov Anton 1
1
 
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland.
Publication typeJournal Article
Publication date2008-11-27
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor1.8
ISSN1540658X, 15578127
PubMed ID:  19035846
Drug Discovery
Molecular Medicine
Abstract
High-throughput screening (HTS) is increasingly being adopted in academic institutions, where the decoupling of screening and drug development has led to unique challenges, as well as novel uses of instrumentation, assay formulations, and software tools. Advances in technology have made automated unattended screening in the 1,536-well plate format broadly accessible and have further facilitated the exploration of new technologies and approaches to screening. A case in point is our recently developed quantitative HTS (qHTS) paradigm, which tests each library compound at multiple concentrations to construct concentration-response curves (CRCs) generating a comprehensive data set for each assay. The practical implementation of qHTS for cell-based and biochemical assays across libraries of > 100,000 compounds (e.g., between 700,000 and 2,000,000 sample wells tested) requires maximal efficiency and miniaturization and the ability to easily accommodate many different assay formats and screening protocols. Here, we describe the design and utilization of a fully integrated and automated screening system for qHTS at the National Institutes of Health's Chemical Genomics Center. We report system productivity, reliability, and flexibility, as well as modifications made to increase throughput, add additional capabilities, and address limitations. The combination of this system and qHTS has led to the generation of over 6 million CRCs from > 120 assays in the last 3 years and is a technology that can be widely implemented to increase efficiency of screening and lead generation.

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Michael S. et al. A Robotic Platform for Quantitative High-Throughput Screening // Assay and Drug Development Technologies. 2008. Vol. 6. No. 5. pp. 637-657.
GOST all authors (up to 50) Copy
Michael S., Auld D., Klumpp C., Jadhav A., Zheng W., Zheng W., Thorne N., Austin C., Inglese J., Simeonov A. A Robotic Platform for Quantitative High-Throughput Screening // Assay and Drug Development Technologies. 2008. Vol. 6. No. 5. pp. 637-657.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1089/adt.2008.150
UR - https://doi.org/10.1089%2Fadt.2008.150
TI - A Robotic Platform for Quantitative High-Throughput Screening
T2 - Assay and Drug Development Technologies
AU - Auld, Douglas
AU - Klumpp, Carleen
AU - Zheng, Wei
AU - Thorne, Natasha
AU - Michael, Sam
AU - Jadhav, Ajit
AU - Zheng, Wei
AU - Austin, Chris
AU - Inglese, J.
AU - Simeonov, Anton
PY - 2008
DA - 2008/11/27 00:00:00
PB - Mary Ann Liebert
SP - 637-657
IS - 5
VL - 6
PMID - 19035846
SN - 1540-658X
SN - 1557-8127
ER -
BibTex |
Cite this
BibTex Copy
@article{2008_Michael
author = {Douglas Auld and Carleen Klumpp and Wei Zheng and Natasha Thorne and Sam Michael and Ajit Jadhav and Wei Zheng and Chris Austin and J. Inglese and Anton Simeonov},
title = {A Robotic Platform for Quantitative High-Throughput Screening},
journal = {Assay and Drug Development Technologies},
year = {2008},
volume = {6},
publisher = {Mary Ann Liebert},
month = {nov},
url = {https://doi.org/10.1089%2Fadt.2008.150},
number = {5},
pages = {637--657},
doi = {10.1089/adt.2008.150}
}
MLA
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MLA Copy
Michael, Sam, et al. “A Robotic Platform for Quantitative High-Throughput Screening.” Assay and Drug Development Technologies, vol. 6, no. 5, Nov. 2008, pp. 637-657. https://doi.org/10.1089%2Fadt.2008.150.
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