Assay and Drug Development Technologies, volume 13, issue 2, pages 66-78

Advances in Laser Scanning Imaging Cytometry for High-Content Screening

WYLIE PAUL G. 1
Onley David J. 1
Hammerstein Anne F. 1
BOWEN WAYNE P. 1
1
 
TTP Labtech Limited, Melbourn, Hertfordshire, United Kingdom.
Publication typeJournal Article
Publication date2015-02-05
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor1.8
ISSN1540658X, 15578127
Drug Discovery
Molecular Medicine
Abstract
The advent of high-content screening more than a decade ago remodeled drug discovery workflows by recasting the role of cell-based approaches in target identification, primary screening, lead optimization, and toxicity. The ability to identify and quantify compound effects on multiple cellular functions allows for rapid characterization of chemical libraries. Laser scanning imaging cytometry (LSIC) is one of the technologies that is being applied to a broad range of assays utilizing fluorescent labeling, at throughputs compatible with primary screening campaigns. Cellular resolution is achieved using laser scanning excitation through a specialized F-theta scan lens. This configuration results in rapid whole well scanning and large depth of field. The recent availability of systems equipped with multiple sources of laser excitation and arrays of detectors for spectral analysis has significantly increased its applicability through enabling more fluorescent reagents and higher levels of multiplexing. LSIC is being used most extensively for phenotypic screening especially in areas such as cell health, RNA interference (RNAi) screening, and three-dimensional cell models. This review communicates advances in LSIC and how it is being applied by presenting an overview of the technology and a range of real-world case studies.

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WYLIE P. G. et al. Advances in Laser Scanning Imaging Cytometry for High-Content Screening // Assay and Drug Development Technologies. 2015. Vol. 13. No. 2. pp. 66-78.
GOST all authors (up to 50) Copy
WYLIE P. G., Onley D. J., Hammerstein A. F., BOWEN W. P. Advances in Laser Scanning Imaging Cytometry for High-Content Screening // Assay and Drug Development Technologies. 2015. Vol. 13. No. 2. pp. 66-78.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1089/adt.2014.607
UR - https://doi.org/10.1089%2Fadt.2014.607
TI - Advances in Laser Scanning Imaging Cytometry for High-Content Screening
T2 - Assay and Drug Development Technologies
AU - WYLIE, PAUL G.
AU - Onley, David J.
AU - Hammerstein, Anne F.
AU - BOWEN, WAYNE P.
PY - 2015
DA - 2015/02/05 00:00:00
PB - Mary Ann Liebert
SP - 66-78
IS - 2
VL - 13
SN - 1540-658X
SN - 1557-8127
ER -
BibTex |
Cite this
BibTex Copy
@article{2015_WYLIE
author = {PAUL G. WYLIE and David J. Onley and Anne F. Hammerstein and WAYNE P. BOWEN},
title = {Advances in Laser Scanning Imaging Cytometry for High-Content Screening},
journal = {Assay and Drug Development Technologies},
year = {2015},
volume = {13},
publisher = {Mary Ann Liebert},
month = {feb},
url = {https://doi.org/10.1089%2Fadt.2014.607},
number = {2},
pages = {66--78},
doi = {10.1089/adt.2014.607}
}
MLA
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MLA Copy
WYLIE, PAUL G., et al. “Advances in Laser Scanning Imaging Cytometry for High-Content Screening.” Assay and Drug Development Technologies, vol. 13, no. 2, Feb. 2015, pp. 66-78. https://doi.org/10.1089%2Fadt.2014.607.
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