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Nucleic Acids Research, volume 49, issue 7, pages 3967-3980

Rif1 regulates telomere length through conserved HEAT repeats

Shubin Calla B 1, 2
Mayangsari Rini 1, 3
Swett Ariel D 1
Greider Carol W. 1, 3
1
 
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
2
 
Biochemistry, Cellular and Molecular Biology Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
3
 
Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, CA 95064, USA
Publication typeJournal Article
Publication date2021-03-27
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
PubMed ID:  33772576
Genetics
Abstract
Abstract In budding yeast, Rif1 negatively regulates telomere length, but the mechanism of this regulation has remained elusive. Previous work identified several functional domains of Rif1, but none of these has been shown to mediate telomere length. To define Rif1 domains responsible for telomere regulation, we localized truncations of Rif1 to a single specific telomere and measured telomere length of that telomere compared to bulk telomeres. We found that a domain in the N-terminus containing HEAT repeats, Rif1177–996, was sufficient for length regulation when tethered to the telomere. Charged residues in this region were previously proposed to mediate DNA binding. We found that mutation of these residues disrupted telomere length regulation even when Rif1 was tethered to the telomere. Mutation of other conserved residues in this region, which were not predicted to interact with DNA, also disrupted telomere length maintenance, while mutation of conserved residues distal to this region did not. Our data suggest that conserved amino acids in the region from 436 to 577 play a functional role in telomere length regulation, which is separate from their proposed DNA binding function. We propose that the Rif1 HEAT repeats region represents a protein-protein binding interface that mediates telomere length regulation.

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Shubin C. B. et al. Rif1 regulates telomere length through conserved HEAT repeats // Nucleic Acids Research. 2021. Vol. 49. No. 7. pp. 3967-3980.
GOST all authors (up to 50) Copy
Shubin C. B., Mayangsari R., Swett A. D., Greider C. W. Rif1 regulates telomere length through conserved HEAT repeats // Nucleic Acids Research. 2021. Vol. 49. No. 7. pp. 3967-3980.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1093/nar/gkab206
UR - https://doi.org/10.1093%2Fnar%2Fgkab206
TI - Rif1 regulates telomere length through conserved HEAT repeats
T2 - Nucleic Acids Research
AU - Shubin, Calla B
AU - Mayangsari, Rini
AU - Swett, Ariel D
AU - Greider, Carol W.
PY - 2021
DA - 2021/03/27 00:00:00
PB - Oxford University Press
SP - 3967-3980
IS - 7
VL - 49
PMID - 33772576
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
Cite this
BibTex Copy
@article{2021_Shubin
author = {Calla B Shubin and Rini Mayangsari and Ariel D Swett and Carol W. Greider},
title = {Rif1 regulates telomere length through conserved HEAT repeats},
journal = {Nucleic Acids Research},
year = {2021},
volume = {49},
publisher = {Oxford University Press},
month = {mar},
url = {https://doi.org/10.1093%2Fnar%2Fgkab206},
number = {7},
pages = {3967--3980},
doi = {10.1093/nar/gkab206}
}
MLA
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MLA Copy
Shubin, Calla B., et al. “Rif1 regulates telomere length through conserved HEAT repeats.” Nucleic Acids Research, vol. 49, no. 7, Mar. 2021, pp. 3967-3980. https://doi.org/10.1093%2Fnar%2Fgkab206.
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