Open Access
Nucleic Acids Research, volume 40, issue 2, pages 712-725
Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria
Patil Shankargouda
1
,
Reyes Aurelio
,
Green Paula
,
Caron Matthieu J
,
Bonini Marcelo G
,
Gordon Donna M.
2
,
Holt Ian J.
,
Santos Janine H.
1
Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, 185 South Orange Avenue, Medical Sciences Building, Newark, NJ 07103, USA.
|
2
, Mississippi State University
|
Publication type: Journal Article
Publication date: 2011-09-26
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
21937513
Genetics
Abstract
Human telomerase reverse transcriptase (hTERT) is localized to mitochondria, as well as the nucleus, but details about its biology and function in the organelle remain largely unknown. Here we show, using multiple approaches, that mammalian TERT is mitochondrial, co-purifying with mitochondrial nucleoids and tRNAs. We demonstrate the canonical nuclear RNA [human telomerase RNA (hTR)] is not present in human mitochondria and not required for the mitochondrial effects of telomerase, which nevertheless rely on reverse transcriptase (RT) activity. Using RNA immunoprecipitations from whole cell and in organello, we show that hTERT binds various mitochondrial RNAs, suggesting that RT activity in the organelle is reconstituted with mitochondrial RNAs. In support of this conclusion, TERT drives first strand cDNA synthesis in vitro in the absence of hTR. Finally, we demonstrate that absence of hTERT specifically in mitochondria with maintenance of its nuclear function negatively impacts the organelle. Our data indicate that mitochondrial hTERT works as a hTR-independent reverse transcriptase, and highlight that nuclear and mitochondrial telomerases have different cellular functions. The implications of these findings to both the mitochondrial and telomerase fields are discussed.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
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Patil S. et al. Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria // Nucleic Acids Research. 2011. Vol. 40. No. 2. pp. 712-725.
GOST all authors (up to 50)
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Patil S., Reyes A., Green P., Caron M. J., Bonini M. G., Gordon D. M., Holt I. J., Santos J. H. Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria // Nucleic Acids Research. 2011. Vol. 40. No. 2. pp. 712-725.
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TY - JOUR
DO - 10.1093/nar/gkr758
UR - https://doi.org/10.1093%2Fnar%2Fgkr758
TI - Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria
T2 - Nucleic Acids Research
AU - Patil, Shankargouda
AU - Reyes, Aurelio
AU - Green, Paula
AU - Caron, Matthieu J
AU - Bonini, Marcelo G
AU - Gordon, Donna M.
AU - Holt, Ian J.
AU - Santos, Janine H.
PY - 2011
DA - 2011/09/26 00:00:00
PB - Oxford University Press
SP - 712-725
IS - 2
VL - 40
PMID - 21937513
SN - 0305-1048
SN - 1362-4962
ER -
Cite this
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Copy
@article{2011_Patil,
author = {Shankargouda Patil and Aurelio Reyes and Paula Green and Matthieu J Caron and Marcelo G Bonini and Donna M. Gordon and Ian J. Holt and Janine H. Santos},
title = {Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria},
journal = {Nucleic Acids Research},
year = {2011},
volume = {40},
publisher = {Oxford University Press},
month = {sep},
url = {https://doi.org/10.1093%2Fnar%2Fgkr758},
number = {2},
pages = {712--725},
doi = {10.1093/nar/gkr758}
}
Cite this
MLA
Copy
Patil, Shankargouda, et al. “Human telomerase acts as a hTR-independent reverse transcriptase in mitochondria.” Nucleic Acids Research, vol. 40, no. 2, Sep. 2011, pp. 712-725. https://doi.org/10.1093%2Fnar%2Fgkr758.