Open Access
Nucleic Acids Research, volume 42, issue 13, pages 8565-8577
Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT
1
University of Colorado BioFrontiers Institute, Boulder, CO 80303, USA Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.
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Publication type: Journal Article
Publication date: 2014-07-02
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
24990373
Genetics
Abstract
Telomerase is the ribonucleoprotein (RNP) enzyme that elongates telomeric DNA to compensate for the attrition occurring during each cycle of DNA replication. Knowing the levels of telomerase in continuously dividing cells is important for understanding how much telomerase is required for cell immortality. In this study, we measured the endogenous levels of the human telomerase RNP and its two key components, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT). We estimate ∼240 telomerase monomers per cell for HEK 293T and HeLa, a number similar to that of telomeres in late S phase. The subunits were in excess of RNPs (e.g. ∼1150 hTR and ∼500 hTERT molecules per HeLa cell), suggesting the existence of unassembled components. This hypothesis was tested by overexpressing individual subunits, which increased total telomerase activity as measured by the direct enzyme assay. Thus, there are subpopulations of both hTR and hTERT not assembled into telomerase but capable of being recruited. We also determined the specific activity of endogenous telomerase and of overexpressed super-telomerase both to be ∼60 nt incorporated per telomerase per minute, with Km(dGTP) ∼17 μM, indicating super-telomerase is as catalytically active as endogenous telomerase and is thus a good model for biochemical studies.
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- We do not take into account publications that without a DOI.
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GOST
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Xi L., Cech T. R. Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT // Nucleic Acids Research. 2014. Vol. 42. No. 13. pp. 8565-8577.
GOST all authors (up to 50)
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Xi L., Cech T. R. Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT // Nucleic Acids Research. 2014. Vol. 42. No. 13. pp. 8565-8577.
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TY - JOUR
DO - 10.1093/nar/gku560
UR - https://doi.org/10.1093%2Fnar%2Fgku560
TI - Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT
T2 - Nucleic Acids Research
AU - Xi, Linghe
AU - Cech, Thomas R.
PY - 2014
DA - 2014/07/02 00:00:00
PB - Oxford University Press
SP - 8565-8577
IS - 13
VL - 42
PMID - 24990373
SN - 0305-1048
SN - 1362-4962
ER -
Cite this
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@article{2014_Xi,
author = {Linghe Xi and Thomas R. Cech},
title = {Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT},
journal = {Nucleic Acids Research},
year = {2014},
volume = {42},
publisher = {Oxford University Press},
month = {jul},
url = {https://doi.org/10.1093%2Fnar%2Fgku560},
number = {13},
pages = {8565--8577},
doi = {10.1093/nar/gku560}
}
Cite this
MLA
Copy
Xi, Linghe, and Thomas R. Cech. “Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT.” Nucleic Acids Research, vol. 42, no. 13, Jul. 2014, pp. 8565-8577. https://doi.org/10.1093%2Fnar%2Fgku560.