Open Access
Nucleic Acids Research, volume 43, issue 18, pages 8964-8972
RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility
Shoji Tatsuma
1
,
Takaya Akiko
1
,
SATO YOSHIHARU
1
,
Kimura Satoshi
2
,
Suzuki Takeo
2
,
Yamamoto Tomoko
1
Department of Microbiology and Molecular Genetics, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-8675, Japan
|
2
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–8656, Japan
|
Publication type: Journal Article
Publication date: 2015-09-13
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
26365244
Genetics
Abstract
Adenine at position 752 in a loop of helix 35 from positions 745 to 752 in domain II of 23S rRNA is involved in binding to the ribosome of telithromycin (TEL), a member of ketolides. Methylation of guanine at position 748 by the intrinsic methyltransferase RlmA(II) enhances binding of telithromycin (TEL) to A752 in Streptococcus pneumoniae. We have found that another intrinsic methylation of the adjacent uridine at position 747 enhances G748 methylation by RlmA(II), rendering TEL susceptibility. U747 and another nucleotide, U1939, were methylated by the dual-specific methyltransferase RlmCD encoded by SP_1029 in S. pneumoniae. Inactivation of RlmCD reduced N1-methylated level of G748 by RlmA(II) in vivo, leading to TEL resistance when the nucleotide A2058, located in domain V of 23S rRNA, was dimethylated by the dimethyltransferase Erm(B). In vitro methylation of rRNA showed that RlmA(II) activity was significantly enhanced by RlmCD-mediated pre-methylation of 23S rRNA. These results suggest that RlmCD-mediated U747 methylation promotes efficient G748 methylation by RlmA(II), thereby facilitating TEL binding to the ribosome.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shoji T. et al. RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility // Nucleic Acids Research. 2015. Vol. 43. No. 18. pp. 8964-8972.
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Shoji T., Takaya A., SATO Y., Kimura S., Suzuki T., Yamamoto T. RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility // Nucleic Acids Research. 2015. Vol. 43. No. 18. pp. 8964-8972.
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TY - JOUR
DO - 10.1093/nar/gkv609
UR - https://doi.org/10.1093%2Fnar%2Fgkv609
TI - RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility
T2 - Nucleic Acids Research
AU - Shoji, Tatsuma
AU - Takaya, Akiko
AU - SATO, YOSHIHARU
AU - Kimura, Satoshi
AU - Suzuki, Takeo
AU - Yamamoto, Tomoko
PY - 2015
DA - 2015/09/13 00:00:00
PB - Oxford University Press
SP - 8964-8972
IS - 18
VL - 43
PMID - 26365244
SN - 0305-1048
SN - 1362-4962
ER -
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@article{2015_Shoji,
author = {Tatsuma Shoji and Akiko Takaya and YOSHIHARU SATO and Satoshi Kimura and Takeo Suzuki and Tomoko Yamamoto},
title = {RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility},
journal = {Nucleic Acids Research},
year = {2015},
volume = {43},
publisher = {Oxford University Press},
month = {sep},
url = {https://doi.org/10.1093%2Fnar%2Fgkv609},
number = {18},
pages = {8964--8972},
doi = {10.1093/nar/gkv609}
}
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MLA
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Shoji, Tatsuma, et al. “RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAIIin 23S rRNA inStreptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility.” Nucleic Acids Research, vol. 43, no. 18, Sep. 2015, pp. 8964-8972. https://doi.org/10.1093%2Fnar%2Fgkv609.