Open Access
Nucleic Acids Research, pages gkw1018
The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli
Temmel Hannes
1
,
Müller Christian
1
,
Sauert Martina
1
,
Vesper Oliver
1
,
Reiss Ariela
,
Popow Johannes
,
Martinez Javier
,
Moll Isabella
1
1
Max F. Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr-Gasse 9/4, A-1030 Vienna, Austria
|
Publication type: Journal Article
Publication date: 2016-10-26
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
PubMed ID:
27789694
Genetics
Abstract
Abstract When Escherichia coli encounters stress, the endoribonuclease MazF initiates a post-transcriptional response that results in the reprogramming of protein synthesis. By removing the 3΄-terminus of the 16S rRNA, MazF generates specialized ribosomes that selectively translate mRNAs likewise processed by MazF. Given the energy required for de novo ribosome biosynthesis, we considered the existence of a repair mechanism operating upon stress relief to recycle the modified ribosomes. Here, we show that the stress-ribosomes and the 3΄-terminal 16S rRNA fragment are stable during adverse conditions. Moreover, employing in vitro and in vivo approaches we demonstrate that the RNA ligase RtcB catalyzes the re-ligation of the truncated 16S rRNA present in specialized ribosomes Thereby their ability to translate canonical mRNAs is fully restored. Together, our findings not only provide a physiological function for the RNA ligase RtcB in bacteria but highlight the reversibility of ribosome heterogeneity, a crucial but hitherto undescribed concept for translational regulation.
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Citations by publishers
1
2
3
4
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4 publications, 20%
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1
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4
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Temmel H. et al. The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli // Nucleic Acids Research. 2016. p. gkw1018.
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Temmel H., Müller C., Sauert M., Vesper O., Reiss A., Popow J., Martinez J., Moll I. The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli // Nucleic Acids Research. 2016. p. gkw1018.
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TY - JOUR
DO - 10.1093/nar/gkw1018
UR - https://doi.org/10.1093%2Fnar%2Fgkw1018
TI - The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli
T2 - Nucleic Acids Research
AU - Temmel, Hannes
AU - Müller, Christian
AU - Sauert, Martina
AU - Vesper, Oliver
AU - Reiss, Ariela
AU - Popow, Johannes
AU - Martinez, Javier
AU - Moll, Isabella
PY - 2016
DA - 2016/10/26 00:00:00
PB - Oxford University Press
SP - gkw1018
PMID - 27789694
SN - 0305-1048
SN - 1362-4962
ER -
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@article{2016_Temmel
author = {Hannes Temmel and Christian Müller and Martina Sauert and Oliver Vesper and Ariela Reiss and Johannes Popow and Javier Martinez and Isabella Moll},
title = {The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli},
journal = {Nucleic Acids Research},
year = {2016},
publisher = {Oxford University Press},
month = {oct},
url = {https://doi.org/10.1093%2Fnar%2Fgkw1018},
pages = {gkw1018},
doi = {10.1093/nar/gkw1018}
}