Open Access
Open access
Nucleic Acids Research, pages gkw1018

The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli

Temmel Hannes 1
Müller Christian 1
Sauert Martina 1
Vesper Oliver 1
Reiss Ariela
Popow Johannes
Martinez Javier
Moll Isabella 1
1
 
Max F. Perutz Laboratories, Center for Molecular Biology, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr-Gasse 9/4, A-1030 Vienna, Austria
Publication typeJournal Article
Publication date2016-10-26
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
PubMed ID:  27789694
Genetics
Abstract
Abstract When Escherichia coli encounters stress, the endoribonuclease MazF initiates a post-transcriptional response that results in the reprogramming of protein synthesis. By removing the 3΄-terminus of the 16S rRNA, MazF generates specialized ribosomes that selectively translate mRNAs likewise processed by MazF. Given the energy required for de novo ribosome biosynthesis, we considered the existence of a repair mechanism operating upon stress relief to recycle the modified ribosomes. Here, we show that the stress-ribosomes and the 3΄-terminal 16S rRNA fragment are stable during adverse conditions. Moreover, employing in vitro and in vivo approaches we demonstrate that the RNA ligase RtcB catalyzes the re-ligation of the truncated 16S rRNA present in specialized ribosomes Thereby their ability to translate canonical mRNAs is fully restored. Together, our findings not only provide a physiological function for the RNA ligase RtcB in bacteria but highlight the reversibility of ribosome heterogeneity, a crucial but hitherto undescribed concept for translational regulation.

Citations by journals

1
2
Journal of Bacteriology
Journal of Bacteriology, 2, 10%
Journal of Bacteriology
2 publications, 10%
Biological Chemistry
Biological Chemistry, 2, 10%
Biological Chemistry
2 publications, 10%
Microbiology spectrum
Microbiology spectrum, 2, 10%
Microbiology spectrum
2 publications, 10%
International Journal of Molecular Sciences
International Journal of Molecular Sciences, 1, 5%
International Journal of Molecular Sciences
1 publication, 5%
Toxins
Toxins, 1, 5%
Toxins
1 publication, 5%
Microorganisms
Microorganisms, 1, 5%
Microorganisms
1 publication, 5%
Science China Life Sciences
Science China Life Sciences, 1, 5%
Science China Life Sciences
1 publication, 5%
Archives of Microbiology
Archives of Microbiology, 1, 5%
Archives of Microbiology
1 publication, 5%
Journal of Biological Chemistry
Journal of Biological Chemistry, 1, 5%
Journal of Biological Chemistry
1 publication, 5%
Microbiological Research
Microbiological Research, 1, 5%
Microbiological Research
1 publication, 5%
Biotechnology and Genetic Engineering Reviews
Biotechnology and Genetic Engineering Reviews, 1, 5%
Biotechnology and Genetic Engineering Reviews
1 publication, 5%
eLife
eLife, 1, 5%
eLife
1 publication, 5%
Current Research in Microbial Sciences
Current Research in Microbial Sciences, 1, 5%
Current Research in Microbial Sciences
1 publication, 5%
Cellular and Molecular Life Sciences
Cellular and Molecular Life Sciences, 1, 5%
Cellular and Molecular Life Sciences
1 publication, 5%
1
2

Citations by publishers

1
2
3
4
American Society for Microbiology
American Society for Microbiology, 4, 20%
American Society for Microbiology
4 publications, 20%
Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 3, 15%
Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 15%
Springer Nature
Springer Nature, 3, 15%
Springer Nature
3 publications, 15%
Elsevier
Elsevier, 2, 10%
Elsevier
2 publications, 10%
Walter de Gruyter
Walter de Gruyter, 2, 10%
Walter de Gruyter
2 publications, 10%
American Society for Biochemistry and Molecular Biology
American Society for Biochemistry and Molecular Biology, 1, 5%
American Society for Biochemistry and Molecular Biology
1 publication, 5%
Taylor & Francis
Taylor & Francis, 1, 5%
Taylor & Francis
1 publication, 5%
eLife Sciences Publications
eLife Sciences Publications, 1, 5%
eLife Sciences Publications
1 publication, 5%
1
2
3
4
  • We do not take into account publications that without a DOI.
  • Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
  • Statistics recalculated weekly.
Metrics
Share
Cite this
GOST |
Cite this
GOST Copy
Temmel H. et al. The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli // Nucleic Acids Research. 2016. p. gkw1018.
GOST all authors (up to 50) Copy
Temmel H., Müller C., Sauert M., Vesper O., Reiss A., Popow J., Martinez J., Moll I. The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli // Nucleic Acids Research. 2016. p. gkw1018.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1093/nar/gkw1018
UR - https://doi.org/10.1093%2Fnar%2Fgkw1018
TI - The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli
T2 - Nucleic Acids Research
AU - Temmel, Hannes
AU - Müller, Christian
AU - Sauert, Martina
AU - Vesper, Oliver
AU - Reiss, Ariela
AU - Popow, Johannes
AU - Martinez, Javier
AU - Moll, Isabella
PY - 2016
DA - 2016/10/26 00:00:00
PB - Oxford University Press
SP - gkw1018
PMID - 27789694
SN - 0305-1048
SN - 1362-4962
ER -
BibTex
Cite this
BibTex Copy
@article{2016_Temmel
author = {Hannes Temmel and Christian Müller and Martina Sauert and Oliver Vesper and Ariela Reiss and Johannes Popow and Javier Martinez and Isabella Moll},
title = {The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli},
journal = {Nucleic Acids Research},
year = {2016},
publisher = {Oxford University Press},
month = {oct},
url = {https://doi.org/10.1093%2Fnar%2Fgkw1018},
pages = {gkw1018},
doi = {10.1093/nar/gkw1018}
}
Found error?