Shock, volume 39, issue 6, pages 543

Mitotracker probes and mitochondrial membrane potential

Kholmukhamedov Andaleb

Schwartz Justin M.

Lemasters John J.

Publication type: Journal Article

Publication date: 2013-05-17

Wolters Kluwer Health

Journal: Shock

Quartile SCImago

Quartile WOS

Impact factor: 3.1

ISSN: 10732322, 15400514

DOI: 10.1097/SHK.0b013e318292300d

Copy DOI

PubMed ID: 23680774

Critical Care and Intensive Care Medicine

Emergency Medicine

Abstract

In the recent article by Lin et al. (1), the authors demonstrate protective effects of splenic infusion of isolated mitochondria against hepatic ischemia/reperfusion injury in a rat model. One of their conclusions is that the isolated mitochondria from donor animals maintained an intact membrane potential in the livers of recipient animals even at 4 hours after infusion, as assessed by Mito-Tracker Orange CMTMRos staining. Cationic fluorophores like rhodamine 123 and tetramethylrhodamine methylester (TMRM) are readily sequestered in the matrix space of polarized mitochondria, and these probes are released once mitochondria experience a loss in membrane potential. MitoTracker dyes are also cationic fluorophores that accumulate electrophoretically into mitochondria in response to the highly negative mitochondrial membrane potential. However, unlike TMRM and rhodamine 123, MitoTracker dyes possess a reactive chloromethyl group that forms a covalent bond with thiols on proteins and peptides, which traps MitoTracker dyes within mitochondria. Thus, mitochondria retain MitoTracker dyes like MitoTracker Orange CMTMRos after loss of their membrane potential (2;3). Hence, retention of MitoTracker staining does not signify that infused mitochondria remain polarized, as was concluded in (1). Indeed, high serum free Ca2+ concentration, which is 10,000 times greater than cytosolic free Ca2+, will lead quickly to mitochondrial Ca2+ overload, respiratory inhibition and mitochondrial dysfunction from onset of the mitochondrial permeability transition with loss of the mitochondrial membrane potential (4;5).

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Citations by publishers

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Springer Nature	Springer Nature, 8, 20% Springer Nature 8 publications, 20%
Wiley	Wiley, 6, 15% Wiley 6 publications, 15%
Elsevier	Elsevier, 5, 12.5% Elsevier 5 publications, 12.5%
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Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 2.5% Royal Society of Chemistry (RSC) 1 publication, 2.5%
Taylor & Francis	Taylor & Francis, 1, 2.5% Taylor & Francis 1 publication, 2.5%
Walter de Gruyter	Walter de Gruyter, 1, 2.5% Walter de Gruyter 1 publication, 2.5%
Rockefeller University Press	Rockefeller University Press, 1, 2.5% Rockefeller University Press 1 publication, 2.5%
Hindawi Limited	Hindawi Limited, 1, 2.5% Hindawi Limited 1 publication, 2.5%
Spandidos Publications	Spandidos Publications, 1, 2.5% Spandidos Publications 1 publication, 2.5%
	1 2 3 4 5 6 7 8

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Kholmukhamedov A., Schwartz J. M., Lemasters J. J. Mitotracker probes and mitochondrial membrane potential // Shock. 2013. Vol. 39. No. 6. p. 543.

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Kholmukhamedov A., Schwartz J. M., Lemasters J. J. Mitotracker probes and mitochondrial membrane potential // Shock. 2013. Vol. 39. No. 6. p. 543.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1097/SHK.0b013e318292300d

UR - https://doi.org/10.1097%2FSHK.0b013e318292300d

TI - Mitotracker probes and mitochondrial membrane potential

T2 - Shock

AU - Kholmukhamedov, Andaleb

AU - Schwartz, Justin M.

AU - Lemasters, John J.

PY - 2013

DA - 2013/05/17 00:00:00

PB - Wolters Kluwer Health

SP - 543

IS - 6

VL - 39

PMID - 23680774

SN - 1073-2322

SN - 1540-0514

ER -

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BibTex Copy

@article{2013_Kholmukhamedov,

author = {Andaleb Kholmukhamedov and Justin M. Schwartz and John J. Lemasters},

title = {Mitotracker probes and mitochondrial membrane potential},

journal = {Shock},

year = {2013},

volume = {39},

publisher = {Wolters Kluwer Health},

month = {may},

url = {https://doi.org/10.1097%2FSHK.0b013e318292300d},

number = {6},

pages = {543},

doi = {10.1097/SHK.0b013e318292300d}

}

MLA

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MLA Copy

Kholmukhamedov, Andaleb, et al. “Mitotracker probes and mitochondrial membrane potential.” Shock, vol. 39, no. 6, May. 2013, p. 543. https://doi.org/10.1097%2FSHK.0b013e318292300d.

Found error?

Publisher

Wolters Kluwer Health

Journal

Shock

Quartile SCImago

Quartile WOS

Impact factor

3.1

ISSN

10732322 (Print)
15400514 (Electronic)