Open Access
Genes and Development, volume 28, issue 19, pages 2077-2089
Regulated assembly and disassembly of the yeast telomerase quaternary complex
1
Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093, USA
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2
Salk Institute for Biological Studies, La Jolla, California 92037, USA.
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3
2Salk Institute for Biological Studies, La Jolla, California 92037, USA
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Publication type: Journal Article
Publication date: 2014-09-19
Journal:
Genes and Development
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 10.5
ISSN: 08909369, 15495477
PubMed ID:
25240060
Genetics
Developmental Biology
Abstract
The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein and two regulatory subunits (Est1 and Est3) in association with the TLC1 RNA, with each of the four subunits essential for in vivo telomerase function. We show here that a hierarchy of assembly and disassembly results in limiting amounts of the quaternary complex late in the cell cycle, following completion of DNA replication. The assembly pathway, which is driven by interaction of the Est3 telomerase subunit with a previously formed Est1-TLC1-Est2 preassembly complex, is highly regulated, involving Est3-binding sites on both Est2 and Est1 as well as an interface on Est3 itself that functions as a toggle switch. Telomerase subsequently disassembles by a mechanistically distinct pathway due to dissociation of the catalytic subunit from the complex in every cell cycle. The balance between the assembly and disassembly pathways, which dictate the levels of the active holoenzyme in the cell, reveals a novel mechanism by which telomerase (and hence telomere homeostasis) is regulated.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Tucey T. M., Lundblad V. Regulated assembly and disassembly of the yeast telomerase quaternary complex // Genes and Development. 2014. Vol. 28. No. 19. pp. 2077-2089.
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Tucey T. M., Lundblad V. Regulated assembly and disassembly of the yeast telomerase quaternary complex // Genes and Development. 2014. Vol. 28. No. 19. pp. 2077-2089.
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TY - JOUR
DO - 10.1101/gad.246256.114
UR - https://doi.org/10.1101%2Fgad.246256.114
TI - Regulated assembly and disassembly of the yeast telomerase quaternary complex
T2 - Genes and Development
AU - Tucey, Timothy M
AU - Lundblad, Victoria
PY - 2014
DA - 2014/09/19 00:00:00
PB - Cold Spring Harbor Laboratory
SP - 2077-2089
IS - 19
VL - 28
PMID - 25240060
SN - 0890-9369
SN - 1549-5477
ER -
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@article{2014_Tucey,
author = {Timothy M Tucey and Victoria Lundblad},
title = {Regulated assembly and disassembly of the yeast telomerase quaternary complex},
journal = {Genes and Development},
year = {2014},
volume = {28},
publisher = {Cold Spring Harbor Laboratory},
month = {sep},
url = {https://doi.org/10.1101%2Fgad.246256.114},
number = {19},
pages = {2077--2089},
doi = {10.1101/gad.246256.114}
}
Cite this
MLA
Copy
Tucey, Timothy M., et al. “Regulated assembly and disassembly of the yeast telomerase quaternary complex.” Genes and Development, vol. 28, no. 19, Sep. 2014, pp. 2077-2089. https://doi.org/10.1101%2Fgad.246256.114.